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The cryopreservation of testicular tissue is presented as the only alternative for the preservation of genetic material from prepubertal animals. However, this biotechnology is still being tested. The objective of this study was to evaluate the effect of different associations of cryoprotectants and the potential of cell proliferation after vitrification of testicular tissue of prepubertal cats. Five testicular pairs from five prepubertal cats were used, and each pair was divided into four fragments. Of these, one fragment composed of the control group (CG) and the rest were distributed in experimental groups according to the associations of cryoprotectants to be tested (dimethyl sulphoxide (DMSO)/glycerol (GLY); ethylene glycol (EG)/GLY) or DMSO/EG) in a final cryoprotectant concentration of 5.6 m. The fragments were submitted to vitrification, and after one week, fragments were heated and processed for histomorphological evaluation and quantification of nucleolar organizer regions (NORs). DMSO/GLY did not differ from CG and was superior to the other vitrified groups, as to cell separation and degree of shrinkage of the basal membrane. Concerning cell differentiation, visibility of the nucleus and nuclear condensation, all the vitrified groups were inferior to CG; however, DMSO/EG was inferior to DMSO/GLY and EG/GLY, which did not differ among themselves. CG was superior to all groups in quantification of NORs. DMSO/EG was inferior to all others, and there was no difference between DMSO/GLY and EG/GLY. The association DMSO/GLY presented the best preservation of tissue integrity and potential of cell proliferation after vitrification of the testicular tissue of prepubertal cats., (© 2016 Blackwell Verlag GmbH.)

Objectives: To evaluate the concentration of kidney injury molecule‐1 and activity of urinary gamma‐glutamyl transferase in cats with urethral obstruction and healthy cats. Materials and Methods: Blood and urine samples were collected from a group of 15 healthy cats (control group) and a group of 20 cats with urethral obstruction at presentation, and 24 hours and 7 days after unblocking the obstruction. The serum creatinine, urinary creatinine and urinary gamma‐glutamyl transferase were measured by spectrophotometry and kidney injury molecule‐1 by the sandwich enzyme‐linked immunosorbent assay. Results: On presentation, cats with obstruction had serum creatinine concentration and urinary gamma‐glutamyl transferase index higher than healthy cats (mean difference 544 μmol/L, 95% confidence intervals 222 to 865 μmol/L, and 0.0022 U/μmol‐uCre, 0.00043 to 0.0039 U/μmol‐uCre, respectively), urine creatinine concentration lower (mean difference 25,624 µmol/L, 17,329 to 33,919 µmol/L), and no significant difference in the kidney injury molecule‐1/urinary creatinine ratio (mean difference 13 pg/μmol‐uCre, −33 to 59 pg/μmol‐uCre). In the group of cats with urinary obstruction, over time serum creatinine decreased, urine creatinine increased, urinary gamma‐glutamyl transferase index did not change significantly, and kidney injury molecule‐1/urinary creatinine ratio increased. Clinical Significance: Cats with post‐renal obstruction and potential intrinsic renal damage had higher urinary gamma‐glutamyl transferase index than healthy cats at the time of presentation and showed increase in kidney injury molecule‐1/urinary creatinine ratio over time. [ABSTRACT FROM AUTHOR]