1. Breaking the Crowther limit: combining depth-sectioning and tilt tomography for high-resolution, wide-field 3D reconstructions.
- Author
-
Hovden, Robert, Ercius, Peter, Jiang, Yi, Wang, Deli, Yu, Yingchao, Abruña, Héctor D, Elser, Veit, and Muller, David A
- Subjects
3D imaging ,Aberration correction ,Catalysts ,Crowther criterion ,Depth of field ,Depth sectioning ,Electron microscopy ,Nanoparticles ,STEM ,Scanning transmission electron microscopy ,TEM ,Through focal imaging ,Tomography ,Nanopartides ,Biomedical Imaging ,Bioengineering ,Generic health relevance ,cond-mat.mtrl-sci ,physics.ins-det ,Microscopy ,Atomic ,Molecular ,Nuclear ,Particle and Plasma Physics ,Optical Physics ,Other Physical Sciences - Abstract
To date, high-resolution (6 nm) to appear blurred or missing. Here we demonstrate a three-dimensional imaging method that overcomes both these limits by combining through-focal depth sectioning and traditional tilt-series tomography to reconstruct extended objects, with high-resolution, in all three dimensions. The large convergence angle in aberration corrected instruments now becomes a benefit and not a hindrance to higher quality reconstructions. A through-focal reconstruction over a 390 nm 3D carbon support containing over 100 dealloyed and nanoporous PtCu catalyst particles revealed with sub-nanometer detail the extensive and connected interior pore structure that is created by the dealloying instability.
- Published
- 2014