1. Highly Efficient Trityl-nitroxide Biradicals for Biomolecular High-field Dynamic Nuclear Polarization
- Author
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Cai, Xinyi, Paioni, Alessandra Lucini, Adler, Agnes, Yao, Ru, Zhang, Wenxiao, Beriashvili, David, Safeer, Adil, Gurinov, Andrei, Rockenbauer, Antal, Song, Yuguang, Baldus, Marc, Liu, Yangping, Sub NMR Spectroscopy, and NMR Spectroscopy
- Subjects
Nitroxide mediated radical polymerization ,Magnetic Resonance Spectroscopy ,Globular protein ,polarizing agents ,chemical and pharmacologic phenomena ,Catalysis ,law.invention ,dynamic nuclear polarization ,law ,Polarization (electrochemistry) ,Spectroscopy ,Electron paramagnetic resonance ,solid-state NMR spectroscopy ,chemistry.chemical_classification ,Biomolecule ,Organic Chemistry ,Membrane Proteins ,General Chemistry ,Small molecule ,biradicals ,Magnetic Fields ,electron paramagnetic resonance ,Solid-state nuclear magnetic resonance ,chemistry ,Chemical physics ,Nitrogen Oxides - Abstract
Dynamic nuclear polarization (DNP) is a powerful method to enhance the sensitivity of solid-state magnetic nuclear resonance (ssNMR) spectroscopy. However, its biomolecular applications at high magnetic fields (preferably > 14 T) have so far been limited by the intrinsically low efficiency of polarizing agents and sample preparation aspects. Herein, we report a new class of trityl-nitroxide biradicals, dubbed SNAPols that combine high DNP efficiency with greatly enhanced hydrophilicity. SNAPol-1, the best compound in the series, shows DNP enhancement factors at 18.8 T of more than 100 in small molecules and globular proteins and also exhibits strong DNP enhancements in membrane proteins and cellular preparations. By integrating optimal sensitivity and high resolution, we expect widespread applications of this new polarizing agent in high-field DNP/ssNMR spectroscopy, especially for complex biomolecules.
- Published
- 2021