1. Methods to Purify and Assay Secretory Pathway Kinases.
- Author
-
Tagliabracci VS, Wen J, and Xiao J
- Subjects
- Animals, Casein Kinase I biosynthesis, Casein Kinase I genetics, Extracellular Matrix Proteins biosynthesis, Extracellular Matrix Proteins genetics, Humans, Sf9 Cells, Spodoptera, Casein Kinase I chemistry, Casein Kinase I isolation & purification, Extracellular Matrix Proteins chemistry, Extracellular Matrix Proteins isolation & purification
- Abstract
Members of the four-jointed and VLK families of secretory pathway kinases appear to be responsible for the phosphorylation of secreted proteins and proteoglycans. These enzymes have been implicated in many biological processes and mutations in several of these kinases cause human diseases. Here, we describe methods to purify and assay two members of the four-jointed family of secretory kinases: the Fam20C protein kinase and the Fam20B proteoglycan kinase.
- Published
- 2016
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