1. A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors.
- Author
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Imbert PR, Louche A, Luizet JB, Grandjean T, Bigot S, Wood TE, Gagné S, Blanco A, Wunderley L, Terradot L, Woodman P, Garvis S, Filloux A, Guery B, and Salcedo SP
- Subjects
- Bacterial Proteins metabolism, Cell Line, Epithelial Cells immunology, Epithelial Cells microbiology, Humans, Pseudomonas aeruginosa immunology, Carrier Proteins antagonists & inhibitors, Immune Evasion, Membrane Glycoproteins antagonists & inhibitors, Myeloid Differentiation Factor 88 antagonists & inhibitors, Pseudomonas aeruginosa pathogenicity, Receptors, Interleukin-1 antagonists & inhibitors, Toll-Like Receptors antagonists & inhibitors, Virulence Factors metabolism
- Abstract
Bacterial pathogens often subvert the innate immune system to establish a successful infection. The direct inhibition of downstream components of innate immune pathways is particularly well documented but how bacteria interfere with receptor proximal events is far less well understood. Here, we describe a Toll/interleukin 1 receptor (TIR) domain-containing protein (PumA) of the multi-drug resistant Pseudomonas aeruginosa PA7 strain. We found that PumA is essential for virulence and inhibits NF-κB, a property transferable to non-PumA strain PA14, suggesting no additional factors are needed for PumA function. The TIR domain is able to interact with the Toll-like receptor (TLR) adaptors TIRAP and MyD88, as well as the ubiquitin-associated protein 1 (UBAP1), a component of the endosomal-sorting complex required for transport I (ESCRT-I). These interactions are not spatially exclusive as we show UBAP1 can associate with MyD88, enhancing its plasma membrane localization. Combined targeting of UBAP1 and TLR adaptors by PumA impedes both cytokine and TLR receptor signalling, highlighting a novel strategy for innate immune evasion., (© 2017 The Authors. Published under the terms of the CC BY 4.0 license.)
- Published
- 2017
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