1. Imaging angiogenesis in patients with head and neck squamous cell carcinomas by [ 68 Ga]Ga-DOTA-E-[c(RGDfK)] 2 PET/CT.
- Author
-
Lobeek D, Rijpkema M, Terry SYA, Molkenboer-Kuenen JDM, Joosten L, van Genugten EAJ, van Engen-van Grunsven ACH, Kaanders JHAM, Pegge SAH, Boerman OC, Weijs WLJ, Merkx MAW, van Herpen CML, Takes RP, Aarntzen EHJG, and Oyen WJG
- Subjects
- Endothelial Cells, Gallium Radioisotopes, Humans, Integrin alphaVbeta3, Positron Emission Tomography Computed Tomography, Squamous Cell Carcinoma of Head and Neck, Tumor Microenvironment, Carcinoma, Squamous Cell diagnostic imaging, Head and Neck Neoplasms diagnostic imaging, Mouth Neoplasms diagnostic imaging
- Abstract
Purpose: Angiogenesis plays an important role in the growth and metastatic spread of solid tumours and is characterised by the expression of integrins on the cell surface of endothelial cells. Radiolabelled RGD peptides specifically target angiogenesis-related α
v β3 integrins, expressed on the activated endothelial cells of sprouting blood vessels. Here, we validated the feasibility of68 Ga[Ga]-DOTA-E-[c(RGDfK)]2 (68 Ga-RGD) PET/CT to visualise angiogenesis in patients with oral squamous cell carcinoma (OSCC)., Methods: Ten patients with OSCC and scheduled for surgical resection including elective neck dissection received an intravenously administration of68 Ga-RGD (42 ± 8 μg; 214 ± 9 MBq). All patients subsequently underwent dynamic (n = 5) or static PET/CT imaging (n = 5) for 60 min or for 4 min/bed position at 30, 60 and 90 min after injection, respectively. Quantitative tracer uptake in tumour lesions was expressed as standardised uptake values (SUV). Additionally, tumour tissue was immunohistochemically stained for αv β3 integrin to assess the expression pattern., Results:68 Ga-RGD tumour accumulation was observed in all patients. At 60 min post injection, tumour SUVmax ranged between 4.0 and 12.7. Tracer accumulation in tumour tissue plateaued at 10 min after injection. Uptake in background tissue did not change over time, resulting in tumour-to-muscle tissue of 6.4 ± 0.7 at 60 min post injection., Conclusions:68 Ga-RGD PET/CT of αv β3 integrin expression in OSCC patients is feasible with adequate tumour-to-background ratios. It will provide more insight in angiogenesis as a hallmark of the head and neck squamous cell carcinomas' tumour microenvironment., Trial Registration: https://eudract.ema.europa.eu no. 2015-000917-31.- Published
- 2020
- Full Text
- View/download PDF