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Start Over Descriptor "miR-1908-3p" Topic carcinogenesis
2 results on '"miR-1908-3p"'

1. Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes.

Author

Zhu Y, Wang Q, Xia Y, Xiong X, Weng S, Ni H, Ye Y, Chen L, Lin J, Chen Y, Niu H, Chen X, and Lin Y

Subjects
Adult, Aged, Aged, 80 and over, Breast Neoplasms genetics, Breast Neoplasms metabolism, Carcinogenesis genetics, Carcinogenesis metabolism, Case-Control Studies, Cell Movement, Cell Proliferation, Female, Follow-Up Studies, Humans, Middle Aged, Neoplasm Metastasis, Prognosis, Tumor Cells, Cultured, Biomarkers, Tumor genetics, Breast Neoplasms pathology, Carcinogenesis pathology, Gene Expression Regulation, Neoplastic, MicroRNAs genetics
Abstract

Background: Breast cancer is one of the most common tumors for women globally. Various miRNAs have been reported to play a crucial role in breast cancer, however the clinical significance of miR-1908-3p in breast cancer remains unclear. The present study aimed to explore the role of miR-1908-3p in breast cancer., Methods: The expression of miR-1908-3p was detected in 50 pairs of breast cancer tissues and adjacent normal tissues, 60 breast cancer patient serum and 60 healthy volunteer serum. The functional roles of miR-1908-3p in breast cancer cells such as proliferation, migration and invasion were evaluated using CCK8, SRB, wound healing and transwell chambers. In addition, bioinformatics tools were used to identify potential targets of miR-1908-3p., Results: The results showed that the expression of miR-1908-3p were increased in breast cancer tissues and serum compared with normal breast tissues and serum of healthy volunteers respectively. Furthermore, the young breast cancer patients and HER2-positive patients had a higher level of tissues' miR-1908-3p than elder breast cancer patients and HER2-negative patients, respectively. The young breast cancer patients had a higher level of serum miR-1908-3p than elder breast cancer patients, ROC analysis suggested that miR-1908-3p had the potential as a promising serum diagnostic biomarker of breast cancer. Up-regulation of miR-1908-3p promoted the cells proliferation, migration and invasion while knockdown of miR-1908-3p inhibited these processes in breast cancer cell MCF-7 and MDA-MB-231. The potential target genes of miR-1908-3p in breast cancer included ID4, LTBP4, GPM6B, RGMA, EFCAB1, ALX4, OSR1 and PPARA. Higher expression of these eight genes correlated with a better prognosis for breast cancer patients., Conclusions: These results suggest that miR-1908-3p may exert its oncogenic functions via suppression of these eight genes in breast cancer.

Published
2020
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2. Evaluation of MiR-1908-3p as a novel serum biomarker for breast cancer and analysis its oncogenic function and target genes

Author

Shuyun Weng, Youzhi Zhu, Yao Lin, Yun Xia, Qingshui Wang, Xiangjin Chen, Xiaoxue Xiong, Junyu Lin, Yan Ye, Haitao Niu, Yajuan Chen, Ling Chen, and Huizhen Ni

Subjects
0301 basic medicine, Oncology, Cancer Research, Carcinogenesis, Proliferation, 0302 clinical medicine, Breast cancer, Invasion, Surgical oncology, Cell Movement, Tumor Cells, Cultured, Neoplasm Metastasis, skin and connective tissue diseases, Migration, Aged, 80 and over, Gene knockdown, Middle Aged, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Prognosis, Gene Expression Regulation, Neoplastic, 030220 oncology & carcinogenesis, Female, Research Article, Adult, medicine.medical_specialty, Breast Neoplasms, lcsh:RC254-282, miR-1908-3p, 03 medical and health sciences, Internal medicine, microRNA, Genetics, medicine, Biomarkers, Tumor, Humans, Clinical significance, Gene, Aged, Cell Proliferation, business.industry, medicine.disease, MicroRNAs, 030104 developmental biology, Case-Control Studies, business, Wound healing, Function (biology), Follow-Up Studies
Abstract

Background Breast cancer is one of the most common tumors for women globally. Various miRNAs have been reported to play a crucial role in breast cancer, however the clinical significance of miR-1908-3p in breast cancer remains unclear. The present study aimed to explore the role of miR-1908-3p in breast cancer. Methods The expression of miR-1908-3p was detected in 50 pairs of breast cancer tissues and adjacent normal tissues, 60 breast cancer patient serum and 60 healthy volunteer serum. The functional roles of miR-1908-3p in breast cancer cells such as proliferation, migration and invasion were evaluated using CCK8, SRB, wound healing and transwell chambers. In addition, bioinformatics tools were used to identify potential targets of miR-1908-3p. Results The results showed that the expression of miR-1908-3p were increased in breast cancer tissues and serum compared with normal breast tissues and serum of healthy volunteers respectively. Furthermore, the young breast cancer patients and HER2-positive patients had a higher level of tissues’ miR-1908-3p than elder breast cancer patients and HER2-negative patients, respectively. The young breast cancer patients had a higher level of serum miR-1908-3p than elder breast cancer patients, ROC analysis suggested that miR-1908-3p had the potential as a promising serum diagnostic biomarker of breast cancer. Up-regulation of miR-1908-3p promoted the cells proliferation, migration and invasion while knockdown of miR-1908-3p inhibited these processes in breast cancer cell MCF-7 and MDA-MB-231. The potential target genes of miR-1908-3p in breast cancer included ID4, LTBP4, GPM6B, RGMA, EFCAB1, ALX4, OSR1 and PPARA. Higher expression of these eight genes correlated with a better prognosis for breast cancer patients. Conclusions These results suggest that miR-1908-3p may exert its oncogenic functions via suppression of these eight genes in breast cancer.

Published
2020

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