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We report results of studies relating to the fabrication of a flexible, disposable, and label free biosensing platform for detection of the cancer biomarker (carcinoembryonic antigen, CEA). Polyaniline (PANI) has been electrochemically deposited over gold sputtered paper (Au@paper) for covalent immobilization of monoclonal carcinoembryonic antibodies (anti-CEA). The bovine serum albumin (BSA) has been used for blocking nonspecific binding sites at the anti-CEA conjugated PANI/Au@Paper. The PANI/Au@Paper, anti-CEA/PANI/Au@Paper, and BSA/anti-CEA/ PANI/Au@Paper platforms have been characterized using scanning electron microscopy, X-ray diffraction, Fourier transmission infrared spectroscopy, chronoamperometry, and electrochemical impedance techniques. The results of the electrochemical response studies indicate that this BSA/ anti-CEA/PANI/Au@paper electrode has sensitivity of 13.9 µA ng–1 ml cm2, shelf life of 22 days, and can be used to estimate CEA in the range of 2–20 ng ml–1. This paper sensor has been validated by detection of CEA in serum samples of cancer patients via immunoassay technique. [ABSTRACT FROM AUTHOR]