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2 results on '"Tan Kean Meng"'

1. Analysis of microalgal growth kinetic model and carbohydrate biosynthesis cultivated using agro-industrial waste residuals as carbon source

Author

Siti Hawa Ramli, Mohd Asyraf Kassim, and Tan Kean Meng

Subjects
biology, Bioconversion, Chemistry, Carbohydrates, Heterotroph, Industrial Waste, Biomass, General Medicine, Wastewater, Raw material, biology.organism_classification, Biochemistry, Carbon, Industrial waste, Tetraselmis suecica, Biofuel, Biofuels, Microalgae, Food science, Sugar, Biotechnology
Abstract

Microalgal carbohydrate is considered one of the potential feedstock for biofuel produced via the bioconversion process. However, the current cultivation process using commercial medium exhibited low biomass production and its carbohydrate productivity which become a bottleneck for sustainable microalgal-carbohydrate-based biofuel production. Thus, the objective of this study is to assess the utilization of industrial waste including molasses and glycerol on the Halochlorella rubescens and Tetraselmis suecica growth as well as its carbohydrate content under different cultivation modes such as autotrophic, heterotrophic and photoheterotrophic conditions. From this study, the highest maximum biomass of H. rubenscens and T. suecica of 0.653 ± 0.009 and 0.669 ± 0.01gL−1 were obtained when the cultivation was performed under photoheterotrophic using molasses. High carbohydrate content of H. rubescens and T. seucica of 56.81 ± 0.39% and 71.52 ± 0.03% with glucose represent the dominant sugar was observed under this condition. The growth kinetic model of the analysis indicated that Huang and Gompertz Models described well the growth of H. rubescens and T. suecica under photoheteroptroph condition with a high significant R2 of 0.99. The information generated could be beneficial for the future development of low-cost microalgal cultivation media formulation for future microalgal carbohydrate-based products such as bioethanol.

Published
2021

2. GROWTH, CARBOHYDRATE PRODUCTIVITY AND GROWTH KINETIC STUDY OF Halochlorella rubescens CULTIVATED UNDER CO2-RICH CONDITIONS.

Author

TAN KEAN MENG and KASSIM, MOHD ASYRAF

Subjects
*CARBOHYDRATES, *INFRARED spectroscopy, *ALGAL growth, *FUNCTIONAL groups, *BIOMASS
Abstract

This study was parametrically established to investigate the effect of different initial pH cultivation medium from pH 4.00 to pH 10.00 and CO2 concentration from 0.04% to 25% (v/v) on the growth and carbohydrate content of Halochlorella rubescens. Changes in biochemical compositions were also analysed using Fourier-transform infrared spectroscopy (FTIR). The maximum concentration of biomass and the productivity carbohydrate were 0.49 ± 0.01 g/L and 22.42 ± 0.03 mg/L.d respectively, when pH 10.00 and 5% (v/v) CO2 concentration were used for cultivation. The FTIR analysis revealed obvious changes in the chemical functional groups for the1200-900 cm-1, 1655 cm-1 and 2850 cm-1 bands, which represent carbohydrate, protein and lipid in microalgal biomass under different cultivation conditions. At the completion of this study, two kinetic growth models, Logistic and Gompertz were evaluated for microalgae growth at elevated condition. The kinetic model analysis for Halochlorella rubescens growth at high CO2 condition fit well with the Gompertz equation with R²value of 0.9977. The data acquired from this research was helpful for predicting the growth characteristics of microalgae in a CO2-rich medium and could act as an essential platform for the production of chemicals and biofuels. [ABSTRACT FROM AUTHOR]

Published
2020

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