1. Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense
- Author
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Brusamarello-Santos, Liziane Cristina, Gilard, Françoise, BRULE, Lenaïg, Quilleré, Isabelle, Gourion, Benjamin, Ratet, Pascal, Maltempi De Souza, Emanuel, Lea, Peter, Hirel, Bertrand, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Department of Biochemistry and Molecular Biology, Mayo Clinic, Centre National de la Recherche Scientifique (CNRS), AgroParisTech, Université Paris-Saclay, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Lancaster Environment Centre, Soil and Ecosystem Ecology Laboratory, National Institute of Science and Technology-Biological Nitrogen Fixation (INCT-FBN) through the Brazilian Research Council (CNPq/MCT) Glenda Sem Fronteiras Program, Brazil, Empresa Brasileira de Pesquisa Agropecuaria (BR), Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, National Institute of Science and Technology-Biological Nitrogen Fixation (INCT-FBN) through the Brazilian Research Council (CNPq/MCT), Ciencia Sem Fronteiras Program, Brazil, Universidade Federal do Paraná (UFPR), Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut des sciences du végétal (ISV), Lancaster University, Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Recherche Agronomique (INRA), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Nitrogen-Fixing Bacteria ,Leaves ,Herbaspirillum ,Nitrogen ,[SDV]Life Sciences [q-bio] ,Carbohydrates ,lcsh:Medicine ,Azospirillum brasilense ,Plant Science ,Diazo Compounds ,Research and Analysis Methods ,Plant Roots ,Zea mays ,Biochemistry ,Model Organisms ,Glucose Metabolism ,Plant and Algal Models ,Metabolites ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Grasses ,Fertilizers ,lcsh:Science ,Plant Biochemistry ,Organic Compounds ,Plant Anatomy ,Organic Chemistry ,lcsh:R ,Organisms ,Chemical Compounds ,Biology and Life Sciences ,Agriculture ,Plants ,Maize ,Chemistry ,Metabolism ,Experimental Organism Systems ,Physical Sciences ,Carbohydrate Metabolism ,lcsh:Q ,Agrochemicals ,Research Article - Abstract
International audience; Maize roots can be colonized by free-living atmospheric nitrogen (N2)-fixing bacteria (diazotrophs). However, the agronomic potential of non-symbiotic N2-fixation in such an economically important species as maize, has still not been fully exploited. A preliminary approach to improve our understanding of the mechanisms controlling the establishment of such N2-fixing associations has been developed, using two maize inbred lines exhibiting different physiological characteristics. The bacterial-plant interaction has been characterized by means of a metabolomic approach. Two established model strains of Nif+ diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense and their Nif- couterparts defficient in nitrogenase activity, were used to evaluate the impact of the bacterial inoculation and of N2 fixation on the root and leaf metabolic profiles. The two N2-fixing bacteria have been used to inoculate two genetically distant maize lines (FV252 and FV2), already characterized for their contrasting physiological properties. Using a well-controlled gnotobiotic experimental system that allows inoculation of maize plants with the two diazotrophs in a N-free medium, we demonstrated that both maize lines were efficiently colonized by the two bacterial species. We also showed that in the early stages of plant development, both bacterial strains were able to reduce acetylene, suggesting that they contain functional nitrogenase activity and are able to efficiently fix atmospheric N2 (Fix+). The metabolomic approach allowed the identification of metabolites in the two maize lines that were representative of the N2 fixing plant-bacterial interaction, these included mannitol and to a lesser extend trehalose and isocitrate. Whilst other metabolites such as asparagine, although only exhibiting a small increase in maize roots following bacterial infection, were specific for the two Fix+ bacterial strains, in comparison to their Fix- counterparts. Moreover, a number of metabolites exhibited a maize-genotype specific pattern of accumulation, suggesting that the highly diverse maize genetic resources could be further exploited in terms of beneficial plant-bacterial interactions for optimizing maize growth, with reduced N fertilization inputs.
- Published
- 2017