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Functionally modified coated capillary is essential for reducing the nonspecific interaction between samples and analytical instruments and improving detection sensitivity and analytical efficiency. Here, we used perfluorooctanoic acid and diazo resin as raw materials, and bonded them to the inner wall of the capillary through the electrostatic interaction of the coupling agent to construct a self-assembled coating tube with anti-fouling properties. The dynamic adsorption test of fluorescent protein confirmed that the coating had obvious anti-protein adsorption effect. The coating was used for capillary electrophoresis (CE) analysis of four mixed protein standard samples and egg white samples. The excellent protein separation spectrum confirmed the application value of the coating. [ABSTRACT FROM AUTHOR]

In this paper, we report on a successful development and application of diazoresin (DR)/β-Cyclodextrin covalently linked capillary coatings using the layer by layer (LBL) self-assembly technique combined with photochemistry reactions. The modified capillary remarkably suppressed the protein adsorption on the inner surface of silica capillary and successfully separated lysozyme (Lys), myoglobin (Mb), bovine serum albumin (BSA) and ribonuclease A (RNase A). Furthermore, the DR/β-Cyclodextrin covalently linked capillary coatings showed good stability and repeatability. More importantly, this method is more environmentally friendly and simpler than traditional methods for the use of DR instead of highly toxic and moisture-sensitive silane coupling agent. [ABSTRACT FROM AUTHOR]

A novel method for the preparation of covalently linked capillary coatings of polystyrene-poly (ethylene oxide) (PS-PEO) was demonstrated by using photosensitive diazoresin (DR) as coupling agents. Layer by layer (LBL) self-assembly membrane of DR and PS-PEO based on hydrogen bonding firstly was fabricated on the inner wall of capillary, then with the treatment of UV light through the unique photochemistry reaction of DR, the hydrogen bonding was converted into covalent bonding. The covalently bonded coatings have the ability of inhibiting protein adsorption on the inner surface of capillary, and thus a baseline separation of Lysozyme (Lys), cytochrome c (Cyt-c), bovine serum albumin (BSA), ribonuclease A (RNase A) and myoglobin (Mb) was achieved by using capillary electrophoresis (CE). Compared with bare capillary or non-covalently bonded DR/PS-PEO coatings, the covalently linked DR/PS-PEO capillary coatings improved the CE separation performance for proteins; what's more, it exhibited good stability and repeatability. This method can provide a green and easy way to make the covalently coated capillaries for CE, replacing of the old way which using highly toxic and moisture sensitive silane coupling agent by DR in the covalent coating preparation. [ABSTRACT FROM AUTHOR]

We reported a new method for preparing coated capillaries in this paper. First, we combined hydroxypropyl cellulose (HPC) and 2-Methacryloyloxyethyl phosphorylcholine (MPC) to form hydroxypropyl cellulose-poly (2-Methacryloyloxyethyl phosphorylcholine) (HPC-PMPC) by atom transfer radical polymerization (ATRP). Then the photosensitive polymer diazoresin (DR) was used as a linker to the inner wall of the capillary, and finally exposed to ultraviolet light to form a stable covalent bond coating. Protein adsorption on the inner surface of capillary was effectively inhibited. The cytochrome c (Cyt-c), lysozyme (Lys), ribonuclease A (RNase A) and bovine serum albumin (BSA) were separated obviously using capillary electrophoresis (CE) within 15 minutes and showed good stability and repeatability. [ABSTRACT FROM AUTHOR]

Covalently linked capillary coating of sodium polystyrene sulfonate (PSS) is prepared using the layer by layer self-assembly technique combined with photochemistry reactions. PSS coating based on ionic bonding was first assembled on the inner wall of capillary by using photosensitive diazoresin (DR) as the coupling agent, and then the ionic bonding was converted into covalent bonding after treatment with UV light through a unique photochemistry reaction of DR. DR/PSS covalent coating resisted protein adsorption on the inner surface of the capillary, and thus a baseline separation of various proteins (Lys, Cyt-c, BSA and RNase A) was achieved in a short time by capillary electrophoresis (CE). Compared with bare capillary and uncrosslinked DR/PSS coating, the covalent coating has good stability and reproducibility. Because of the replacement of highly toxic and moisture-sensitive silane coupling agent by DR in the covalent coating preparation, this method may provide a green and easy way to make the covalently coated capillaries for CE. [ABSTRACT FROM AUTHOR]

Protein adsorption on the inner surface of capillary is the main reason for the poor separation performance of capillary electrophoresis. Currently, many ways have been reported to inhibit protein adsorption, while they have numerous disadvantages, such as complicated, toxic and unstable. Here, by using photosensitive diazoresin (DR) as coupling agents and graphene oxide (GO) as coating, we found a simple, economic and environmental friendly approach to inhibit protein adsorption. Through layer by layer (LBL) self-assembly technique and unique photochemistry reaction of DR, a stable and efficient capillary coating could be obtained. The bare capillary, non-covalently GO coated capillary and covalently GO coated capillary were all characterized. Results indicated that the DR/GO covalently linked capillary coatings showed great separation performance of proteins with excellent stability and repeatability. Compared with bare capillary and non-covalent, better separation efficiency was achieved by covalent GO capillary column. [ABSTRACT FROM AUTHOR]

The ability to manipulate and control the inner surface properties of silica capillary is of crucial importance for analysis of proteins by capillary electrophoresis. In this work, surface-initiated atom transfers radical polymerization (ATRP) of zwitterionic 3-dimethyl(methacryloyloxyethyl) ammonium propane sulfonate (DMAPS) is employed to tailor the functionality of silica capillary in a well-controlled manner. The structure of the coating and the protein separation performance were investigated. The results confirmed that the P(DMAPS) grafting can substantially enhance the performance of the capillary columns. With the versatility of surface-initiated ATRP and the excellent performance of zwitterionic polymer chains, the zwitterionic polymer coated capillary columns can be readily tailored for analysis of proteins by capillary electrophoresis. [ABSTRACT FROM AUTHOR]

Capillary electrophoresis has become an effective tool for the separation and analysis of biomolecule macromolecule.In this work, a new method to prepare covalent connection capillary electrophoretic coatings is proposed. Photosensitive diazoresin (DR) as coupling agents and carboxymethyl chitosan as coating was used. Through layer by layer (LBL) self-assembly and unique photochemistry reaction of DR, a stable and efficient capillary coating can be obtained. The covalently bonded coatings have the ability of suppressing protein adsorption on the inner surface of silica capillary, and thus a baseline separation of Lysozyme (Lys), myoglobin (Mb), bovine serum albumin (BSA) and ribonuclease A (RNase A) can be achieved. The covalently linked DR/carboxymethyl chitosan capillary coatings presented good stability and repeatability. This method seems to be an environmental and simple way to prepare the covalently coated capillaries for CE. [ABSTRACT FROM AUTHOR]

Considering the conventional silane coupling agents in the surface modification of the application of toxicity, moisture sensitive and other defects. We proposed a new method of preparing an environmentally friendly capillary coating by using a photosensitive diazoresin (DR) and poly(ethylene oxide)x-b-polystyrene-b-poly(ethylene oxide)x (PEOx-b-PS-b-PEOx, x = 1, 2, 3) was coated on the inner surface of the capillary, and the separation performance of the PEOx-b-PS-b-PEOx capillary coating was investigated thoroughly. The coating shields the adsorption of protein on the inner wall of the capillary and baseline separation of the four proteins of lysozyme (Lys), cytochrome c (Cyt-c), bovine serum albumin (BSA) and ribonuclease A (RNase A) can be realized within 20 minutes. [ABSTRACT FROM AUTHOR]

Covalent capillary coatings of diazoresin (DR) and rapamycin (RAPA) were prepared by a layer-by-layer self-assembly. DR worked as the coupling agent. Under ultraviolet light, the corsslinking was carried out by covalent bonds between DR and RAPA. The covalent capillary coatings could suppress protein adsorption. The baseline separation of lysozyme (Lys), cytochrome c (Cyt-c), bovine serum albumin (BSA) and myoglobin (Mb) could be achieved within 17 minutes by using capillary electrophoresis (CE). The covalent capillary coatings exhibited good chemical stability and repeatability. In the synthesis, highly toxic moisture-sensitive silane has been replaced by DR. The method provides an environmentally friendly way to prepare the covalent capillary coating. [ABSTRACT FROM AUTHOR]

A novel method to prepare capillary coatings using dopamine (DA) and hyaluronic acid (HA) was mentioned in this paper. Firstly, dopamine was oxidized to form polydopamine and attached to the inner wall of the capillary spontaneously. HA attached to the polydopamine by electrostatic interaction to form a polydopamine-hyaluronic acid (PDA-HA) coating. Protein adsorption on the inner surface of capillary was suppressed, and the proteins of lysozyme (Lys), cytochrome c (Cyt-c), bovine serum albumin (BSA) and ribonuclease A (RNase A) were baseline separated obviously using capillary electrophoresis (CE) within 20 minutes. [ABSTRACT FROM PUBLISHER]

A simple, environmental and economical capillary electrophoresis method has been developed for the analysis of four model proteins by employing a photoactive and non-toxic diazoresin (DR), D-Glucurone modification of the inner surface of fused-silica capillary. DR/D-Glucurone coated capillary not only effectively eliminated the wall adsorption of proteins, but also showed good stability and repeatability. Instead of using the moisture sensitive and toxic silane coupling agent, the application of photosensitive DR may provide a flexible and environmentally friendly strategy to prepare novel covalently coated capillaries. Thus, DR/D-Glucurone coated capillary has great potential in capillary electrochromatography and may provide a new method for biological compounds separation. [ABSTRACT FROM AUTHOR]

In this paper, we demonstrated a novel method for the preparation of covalently bonded capillary coatings of polyvinyl alcohol (PVA). Layer by layer (LBL) self-assembly film of DR and PVA based on hydrogen bonding was fabricated on the inner wall of capillary. The hydrogen bonding was converted into covalent bonding after treatment with UV light through the unique photochemistry reaction of DR. The covalently bonded coatings suppressed basic protein adsorption on the inner surface of capillary, and a baseline separation of three basic proteins – lysozyme (Lys), cytochrome c (Cyt-c) and bovine serum albumin (BSA) was achieved using capillary electrophoresis (CE). [ABSTRACT FROM AUTHOR]

A novel method for the preparation of covalently bonded capillary coatings of polyethylene glycol (PEG) was demonstrated. Layer by layer self-assembly film of diazoresin (DR) and PEG based on hydrogen bonding was fabricated on the inner wall of capillary. The hydrogen bonding was converted into covalent bonding after treatment with UV light through the unique photochemistry reaction of DR. The covalently bonded coatings suppressed protein adsorption on the inner surface of the capillary, and a baseline separation of three basic proteins was achieved using capillary electrophoresis (CE). [ABSTRACT FROM AUTHOR]