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Unmanned aerial vehicles (UAV) allow efficient acquisition of forest data at very high resolution at relatively low cost, making it useful for multi-temporal assessment of detailed tree crowns and forest structure. Single-pass flight plans provide rapid surveys for key selected high-priority areas, but their accuracy is still unexplored. We compared aircraft-borne LiDAR with GatorEye UAV-borne LiDAR in the Apalachicola National Forest, USA. The single-pass approach produced digital terrain models (DTMs), with less than 1 m differences compared to the aircraft-derived DTM within a 145° field of view (FOV). Canopy height models (CHM) provided reliable information from the top layer of the forest, allowing reliable treetop detection up to wide angles; however, underestimations of tree heights were detected at 175 m from the flightline, with an error of 2.57 ± 1.57. Crown segmentation was reliable only within a 60° FOV, from which the shadowing effect made it unviable. Reasonable quality threshold values for LiDAR products were: 195 m (145° FOV) for DTMs, 95 m (110° FOV) for CHM, 160 to 180 m (~140° FOV) for ITD and tree heights, and 40 to 60 m (~60° FOV) for crown delineation. These findings also support the definition of mission parameters for standard grid-based flight plans under similar forest types and flight parameters. [ABSTRACT FROM AUTHOR]