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A survey of host trees infested with eastern mistletoe (Phoradendron leucarpum, Viscaceae) at General Burnside State Park in Pulaski County, Kentucky, was made in late 2008 and early 2009. It is the only island state park in Kentucky, and consists of 174.0 ha in the middle of Lake Cumberland, adjacent to the city of Burnside. A total of 244 mistletoe-infested trees from nine tree species in eight families were found. Prunus serotina, Juglans nigra, and Ulmus americana were the most common host trees. Mistletoe infestation had a greater occurrence in older, full-crowned canopy trees of open, exposed sunny habitats.