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Your search keyword '"Hai Ming Xin"' showing total 3 results
Start Over Author "Hai Ming Xin" Topic cancer research
3 results on '"Hai Ming Xin"'

2. Effect of RNA interference of iASPP on the apoptosis in MCF-7 breast cancer cells

Author

Xing Gao, Lu Hou, Yun Cai, Hai-Ming Xin, Shan Zhong, Shou-Zhi Gu, Ze-Jun Liu, Xin Lu, and Jie Chen

Subjects
Cancer Research, Apoptosis, Breast Neoplasms, Biology, Transfection, Green fluorescent protein, law.invention, Western blot, RNA interference, law, Cell Line, Tumor, medicine, Humans, medicine.diagnostic_test, fungi, Intracellular Signaling Peptides and Proteins, General Medicine, Repressor Proteins, Oncology, MCF-7, Protein Biosynthesis, Cancer research, Recombinant DNA, RNA Interference, Breast cancer cells, Tumor Suppressor Protein p53, Plasmids
Abstract

ASPP family is proved to be apoptotic specific regulators of p53. Among them, iASPP acts as an inhibitor of p53. To investigate the effect of the iASPP RNAi on the apoptosis of breast cancer cell MCF-7, we transfected the recombinant plasmid PGCsilencer H1/Neo/GFP/RNAi into MCF-7. The iASPP expression was analyzed by RT-PCR and Western blot. The cell apoptosis was detected by FCM. The results show that the expression of iASPP is descended and the apoptosis rate is increased after transfection. Therefore, we conclude that inhibition of expression of iASPP may resume the ability of p53 to induce apoptosis in MCF-7 cells.

Published
2016

3. Downregulated mRNA expression of ASPP and the hypermethylation of the 5'-untranslated region in cancer cell lines retaining wild-type p53

Author

Xiao-Bing Zhang, Shan Zhong, Yun Zhang, Shou-Zhi Gu, Xin Lu, Ze-Jun Liu, Hai-Ming Xin, and Xin Yang

Subjects
Untranslated region, Transcriptional Activation, p53, Five prime untranslated region, Bisulfite sequencing, Molecular Sequence Data, Biophysics, Down-Regulation, Apoptosis, Biology, Biochemistry, Structural Biology, Cell Line, Tumor, Genetics, Humans, RNA, Messenger, Molecular Biology, Gene, Adaptor Proteins, Signal Transducing, DNA methylation, Base Sequence, Reverse transcription PCR, Wild type, Cell Biology, Molecular biology, Reverse transcription polymerase chain reaction, Gene Expression Regulation, Neoplastic, CpG site, Methylation-specific PCR, Cancer research, CpG Islands, Tumor Suppressor Protein p53, 5' Untranslated Regions, Apoptosis Regulatory Proteins, Carrier Proteins, ASPP2, ASPP1
Abstract

The p53 protein is one of the best-known tumour suppressors. Recently discovered ASPP1 and ASPP2 are specific activators of p53. To understand, if apoptosis-stimulating protein of p53 (ASPP) inactivation offers a selective advantage to tumors that have wild-type p53, we measured the mRNA expression of ASPP1 and ASPP2 in tumor cell lines retaining wide-type p53. In addition, the CpG island methylation status of ASPP1 gene and ASPP2 gene in the 5′-untranslated region was also investigated in order to understand the possible cause of abnormal expression of ASPP1 and ASPP2 in the tumor cell lines retaining wide-type p53. The data showed that mRNA expression of ASPP1 and ASPP2 is downregulated and CpG island tested is hypermethylated. These results indicated that ASPP CpG island aberrant methylation could be one molecular and genetic alteration in wild-type p53 tumours.

Published
2005

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