1. Interference-free SERS nanoprobes for labeling and imaging of MT1-MMP in breast cancer cells.
- Author
-
Zhu, Dan, Li, Anran, Di, Yunsong, Wang, Zhuyuan, Shi, Jingzhan, Ni, Xiaoqi, and Wang, Yiping
- Subjects
CANCER cells ,BREAST cancer ,SERS spectroscopy ,BREAST imaging ,CHEMICAL fingerprinting - Abstract
The expression of membrane type-1 matrix metalloproteinase (MT1-MMP) in cancer cells is critical for understanding the development, invasion and metastasis of cancers. In this study, we devised an interference-free surface-enhanced Raman scattering (SERS) nanoprobe with high selectivity and specificity for MT1-MMP. The nanoprobe was comprised of silver core-silica shell nanoparticle with a Raman reporter tag (4-mercaptobenzonitrile) embedded in the interface. Moreover, the nitrile group in 4-mercaptobenzonitrile shows a unique characteristic peak in the Raman-silent region (1800â€"2800 cm
â'1 ), which eliminates spectral overlapping or background interference in the Raman fingerprint region (500â€"1800 cmâ'1 ). After surface modification with a targeting peptide, the nanoprobe allowed visualization and evaluation of MT1-MMP in breast cancer cells via SERS spectrometry. This interference-free, peptide-functionalized SERS nanoprobe is supposed to be conducive to early diagnosis and invasive assessment of cancer in clinical settings. [ABSTRACT FROM AUTHOR]- Published
- 2022
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