1. Induction of G1 phase arrest and apoptosis in MDA-MB-231 breast cancer cells by troglitazone, a synthetic peroxisome proliferator-activated receptor γ (PPARγ) ligand
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Yu, Hong-Nu, Lee, Young-Rae, Noh, Eun-Mi, Lee, Kyung-Sun, Kim, Jong-Suk, Song, Eun-Kyung, Han, Myung-Kwan, Lee, Yong-Chul, Kwon, Kang-Beom, Lee, Seung-Jin, Youn, Hyun Jo, and Jung, Sung Hoo
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PEROXISOMES ,APOPTOSIS ,CANCER cells ,LIGANDS (Biochemistry) - Abstract
Abstract: Peroxisome proliferator-activated receptor γ (PPARγ) ligands inhibit cell proliferation and induce apoptosis in cancer cells. Here we wished to determine whether the PPARγ ligand induces apoptosis and cell cycle arrest of the MDA-MB-231 cell, an estrogen receptor α negative breast cancer cell line. The treatment of MDA-MB-231 cell with PPARγ ligands was shown to induce inhibition of cell growth in a dose-dependent manner as determined by MTT assay. Cell cycle analysis showed a G
1 arrest in MDA-MB-231 cells exposed to troglitazone. An apoptotic effect by troglitazone demonstrated that apoptotic cells elevated by 2.5-fold from the control level at 10μM, to 3.1-fold at 50μM and to 3.5-fold at 75μM. Moreover, troglitazone treatment, applied in a dose-dependent manner, caused a marked decrease in pRb, cyclin D1 , cyclin D2 , cyclin D3 , Cdk2, Cdk4 and Cdk6 expression as well as a significant increase in p21 and p27 expression. These results indicate that troglitazone causes growth inhibition, G1 arrest and apoptotic death of MDA-MB-231 cells. [Copyright &y& Elsevier]- Published
- 2008
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