1. Effects of borneol and thymoquinone on TNBS-induced colitis in mice.
- Author
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Juhás S, Cikos S, Czikková S, Veselá J, Il'ková G, Hájek T, Domaracká K, Domaracký M, Bujnáková D, Rehák P, and Koppel J
- Subjects
- Animals, Body Weight drug effects, Colon drug effects, Colon pathology, Cytokines genetics, Cytokines metabolism, DNA metabolism, DNA Restriction Enzymes metabolism, Gene Expression Regulation drug effects, Male, Mice, Organ Size drug effects, RNA, Messenger genetics, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Benzoquinones pharmacology, Camphanes pharmacology, Colitis chemically induced, Colitis pathology, Trinitrobenzenesulfonic Acid pharmacology
- Abstract
Components of plant essential oils have been reported to have health benefit properties, including antioxidative, anti-tumour, antimicrobial, anti-stress, and immunomodulative activities. We examined the anti-inflammatory effects of thymoquinone, the active ingredient in the volatile oil of Nigella sativa seeds, and borneol, the active component of Salvia officinalis essential oil, on TNBS-induced colitis in mice. Thymoquinone was added to the commercial diet at a concentration of 0.05 % and borneol at two concentrations (0.09% and 0.18%) and fed to ICR mice 5 days before induction of TNBS colitis. Seven days after TNBS administration the mice were killed and macroscopic and histological scores were evaluated. Cytokine mRNA expression in colonic tissue was assessed using quantitative realtime RT-PCR. We did not detect any significant changes in macroscopic and histological scores between experimental and control groups, but we observed a significant decrease in proinflammatory cytokine (IL-1beta and IL-6) mRNA expression in colon tissue in the 0.09% and 0.18% borneol-treated groups of mice in comparison to the control group. Surprisingly, we were not able to confirm anti-inflammatory effects of thymoquinone in TNBS colitis. In conclusion, our data show that borneol is able to significantly suppress proinflammatory cytokine mRNA expression in colonic inflammation, although no significant morphological changes are visible.
- Published
- 2008