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Submitted by (edna.saturno@ufrpe.br) on 2017-02-23T14:03:29Z No. of bitstreams: 1 Juliana Maria Aderaldo Vidal.pdf: 784041 bytes, checksum: f927beb63f5c3c304d4098214b0dd629 (MD5) Made available in DSpace on 2017-02-23T14:03:29Z (GMT). No. of bitstreams: 1 Juliana Maria Aderaldo Vidal.pdf: 784041 bytes, checksum: f927beb63f5c3c304d4098214b0dd629 (MD5) Previous issue date: 2015-07-27 Bacteria with probiotic effect have been used in marine shrimp production like antibiotics substitution, contributing to the health of the host, by antagonistic action to pathogenic micro-organisms or by competition for space and nutrients, and improving appetite and lead the further growth of the animals. When isolated from the host itself is safe to use, being able to adhere and colonize the intestine. The objective was to isolate probiotic bacteria from intestine of juvenile Litopenaeus vannamei and evaluate the effects of probiotic administration in the performance of cultivated animals experimental challenged infection. Intestinal bacteria were from cultivated shrimp in oligohaline water and saltwalter in dry and rainy seasons, and confronted with Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus and Aeromonas hydrophila. Bacteria showed antimicrobial effect have been identified by molecular biology. Twenty-nine isolates showed antagonistic effect at least one of the tested pathogens. The species that most occurred was Bacillus cereus, the producing of the largest zones of inhibition to the V. alginolyticus and V. vulnificus. The bacterium Citrobacter freundii, from oligohaline water showed the best antimicrobial effect before the four pathogens. The probiotic Bacillus cereus was tested in diet for marine shrimp post-larvae against challenge with V. parahaemolyticus, and V. alginolyticus which evaluated the growth performance of animals, colonization capacity of the probiotic bacteria, pathogens count and histopathological lesions. The use of probiotic had no effect on animals survival, but not for treatments which was used probiotic had a lower weight gain. Animals fed dietary supplementation of probiotic had lower pathogens count those fed without the use. Histopathological lesions were observed in organs and tissues of animals. It can be concluded that it was possible to isolate bacteria having probiotic effect of marine shrimp intestine, wherein the strain Bacillus cereus demonstrated high capacity to colonize the host itself, causing a significant reduction of pathogens. Bactérias com efeito probiótico contribuem para saúde de camarões marinhos em cultivo, em substituição aos antibióticos, exercendo ação antagonista à micro-organismos patogênicos ou por competição por espaço e nutrientes, além de melhorar o apetite levando a um maior crescimento dos animais. Quando isolados do próprio camarão seu uso é seguro, por serem capazes de aderir ou colonizar o intestino destes. Neste sentido, objetivou-se isolar bactérias probióticas do intestino de juvenis de Litopenaeus vannamei e avaliar os efeitos da sua administração sobre o desempenho dos animais cultivados frente infecção experimental. Foram isoladas bactérias do intestino de camarão cultivado em águas oligohalina e salgada, em períodos seco e chuvoso, e confrontadas com Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio alginolyticus e Aeromonas hydrophila, em testes in vitro. As bactérias que produziram efeito antimicrobiano foram identificadas através de biologia molecular.Vinte e nove isolados apresentaram efeito antagônico a pelo menos um dos patógenos testados. A espécie que mais ocorreu foi Bacillus cereus, produzindo os maiores halos de inibição frente ao V. alginolyticus e V. vulnificus. A bactéria Citrobacter freundii, proveniente de água oligohalina apresentou melhor efeito antimicrobiano, perante os quatro patógenos. O probiótico Bacillus cereus foi testado em ração para pós-larvas de L. vannamei, frente a desafio com V. parahaemolyticus, e V. alginolyticus quando avaliou-se o desempenho zootécnico dos animais, a capacidade de colonização das bactérias probióticas, contagem de patógenos e lesões histopatológicas. O uso do probiótico não influenciou nas taxas de sobrevivência dos animais, porém nos tratamentos em que não se utilizou probiótico houve menor ganho de peso. Os animais que receberam ração suplementada de probiótico, tiveram contagem de patógenos inferior aqueles alimentados sem o uso. Não foram observadas lesões histopatológicas nos órgãos e tecidos dos animais. Concluiu-se que é possível isolar bactérias com efeito probiótico do intestino do camarão marinho, sendo que a cepa de Bacillus cereus demonstrou maior capacidade de colonizar o próprio hospedeiro, diminuindo os patógenos.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-13T11:45:58Z No. of bitstreams: 1 Fabiana Penalva de Melo.pdf: 587502 bytes, checksum: 899b9b2cc09b866a034d428f2e22e935 (MD5) Made available in DSpace on 2017-02-13T11:45:58Z (GMT). No. of bitstreams: 1 Fabiana Penalva de Melo.pdf: 587502 bytes, checksum: 899b9b2cc09b866a034d428f2e22e935 (MD5) Previous issue date: 2012-02-03 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES This study aimed to evaluate the culture of Litopenaeus vannamei marine shrimp fed with different protein levels diets in heterotrophic systems with and without probiotic addition. It was adopted a completely randomized design with 4 x 2 factorial arrangement, using four dietary protein levels (200, 250, 300 e 350 g Kg-1 CP), as the first factor (P20, P25, P30 and P35), and probiotic addition in the water, as the second factor (P20Pro, P25Pro P30Pro e P35Pro). For this were used 24 fiberglass tanks (800 L working volume), filled with a mixture of 300 liters of seawater (30 g L-1), chlorinated and dechlorinated, and 500 liters of seawater from the before culture. Each tank was stocked with 240 juveniles (300 shrimp m-3) averaging 1.55±0.1 g. Water temperature, dissolved oxygen, pH and salinity were measured daily, while NH3-N, NO2-N, NO3-N, PO4-P and alkalinity were analyzed weekly. There were no significant differences in daily parameters of water quality (temperature, dissolved oxygen, pH and salinity; P>0.05), while the weekly parameters only nitrite was influenced by the protein levels (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-07T11:34:15Z No. of bitstreams: 1 Alexandre Duarte Rodrigues da Silva.pdf: 439100 bytes, checksum: 1b2fd1350f22fda3959acac92bbccf28 (MD5) Made available in DSpace on 2017-02-07T11:34:15Z (GMT). No. of bitstreams: 1 Alexandre Duarte Rodrigues da Silva.pdf: 439100 bytes, checksum: 1b2fd1350f22fda3959acac92bbccf28 (MD5) Previous issue date: 2010-06-14 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Culture of Litopenaeus vannamei in freshwater (0.0 g L-1) and low alkalinity and hardness (

Submitted by (ana.araujo@ufrpe.br) on 2016-08-05T13:36:28Z No. of bitstreams: 1 Marcelle Marques da Silva.pdf: 960120 bytes, checksum: 2630be7e6c34db10ff7c5fedf307426e (MD5) Made available in DSpace on 2016-08-05T13:36:28Z (GMT). No. of bitstreams: 1 Marcelle Marques da Silva.pdf: 960120 bytes, checksum: 2630be7e6c34db10ff7c5fedf307426e (MD5) Previous issue date: 2009-02-19 The objective to maximize the survival of the post-larvae (PL) of the marine shrimp Litopenaeus vannamei being related with the variable of handling (density of stockage of the post-larvae, month of culture, days of culture, nurseries and laboratories supplying of the post-larvae) when cultivated in the nurseries. They had been 65 data of culture of a commercial farm located in the state of Rio Grande do Norte corresponding to the period from october 2004 to march 2007. The post-larvae (PL) were storage in nursery with densities varied from 12 to 64 PL/L in a period of five to 19 days. The system of culture adopted in the nursery phase was the intensive with capacity of 50.000 liters, with system of aeration and draining independents. The supplying of the tanks was made using water of the canal of supplying of the fisheries. The feeding used was to the base of biomass of artêmia and commercial ration with 40% of rude protein, and was managed of daily of two in two hours. To relate the survival of the post-larvae with the culture variable it was used multiple regression, where its parameters had been esteem on the base of the minimization the sum of square of residues. The selection of variable was carried through using the process of Stepwise Forward, establishing value 3 of statistics “F” of Snedecor of entrance and exit, associated the Montgomery and Peck to minimize the sum of square of residues. To verify the adequacy of the modelto the data, the linearity,normality and homogeneity for the residues had been the analysis of variance (ANOVA) and analysis residues. Thus, to the shape a survival of the post-larvae of the marine shrimp Litopenaeus vannamei in the nursery phase, with the variable of handling, it was gotten R2= 60,81%, and through the ANOVA is verified that the level of probability of rejection of the model was very small. It was concluded that we can shape the survival with the culture parameters, where the origin of the post-larvae, the time of culture and the density influence of significant form inthe survival and send regards to the producer that it uses PL of the laboratories LFPL2, LFPL3, LFPL4 e LFPL7, therefore with certainty it will get success in its production. Objetivou-se maximizar a sobrevivência das pós-larvas (PL) do camarão marinho Litopenaeus vannamei relacionando-a com as variáveis de manejo (densidade de estocagem das pós-larvas, mês de cultivo, tempo de cultivo, tanques berçários e laboratórios fornecedores das pós-larvas), quando cultivadas em tanques berçários. Foram utilizados 65 dados de cultivos de uma fazenda comercial, localizada no estado do Rio Grande do Norte, correspondente ao período de outubro de 2004 a março de 2007. As PL foram estocadas em tanques berçários às densidades que variaram de 12 a 64 PL/L, durante um período de cinco a 19 dias. O sistema de cultivo adotado foi o intensivo em tanques com capacidade de 50.000L, com sistema de aeração e drenagem independentes. O abastecimento dos tanques foi feito utilizando-se água do canal de abastecimento dos viveiros. A alimentação utilizada foi à base de biomassa de artêmia e ração comercial com 40% de proteína bruta, administrada diariamente de duas em duas horas. Para relacionar a sobrevivência das PL com as variáveis de cultivo utilizou-se a regressão múltipla, em que seus parâmetros foram estimados com base na minimização da soma dos quadrados. A seleção de variáveis foi realizada utilizando-se o processo StepwiseForward estabelecendo o valor três da estatística “F” de Snedecor de entrada (Fentrada) e de saída (Fsaída), associado com a técnica de Montgomery e Peck para minimizar a soma dos quadrados dos resíduos. Para verificar a adequação do modelo aos dados, a linearidade, normalidade e homogeneidade dos resíduos foram utilizados a análise de variância (ANOVA) e a análise de resíduos. Assim, ao modelar a sobrevivência (Sob-%) das PL com as variáveis de manejo, obteve-se R2= 60,81% e através da ANOVA verificou-se que o nível de probabilidade de rejeição do modelo foi muito pequeno. Verifica-se que podemos modelar a sobrevivência com os parâmetros de cultivo, onde a procedência das PL, o tempo de cultivo e a densidade influem de forma significativa na sobrevivência, e recomenda-se ao produtor que utilize PL dos laboratórios LFPL2, LFPL3, LFPL4 e LFPL7, pois com certeza ele obterá sucesso no seu agronegócio.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-13T12:20:42Z No. of bitstreams: 1 Joanna Dourado.pdf: 721470 bytes, checksum: 1ade18b6e4af854fcb0abb1162f7c2bb (MD5) Made available in DSpace on 2016-10-13T12:20:42Z (GMT). No. of bitstreams: 1 Joanna Dourado.pdf: 721470 bytes, checksum: 1ade18b6e4af854fcb0abb1162f7c2bb (MD5) Previous issue date: 2009-02-13 Shrimp farming all over the world has been affected in the last years by many diseases, that have contributed to the development indexes decline in the production of farmed marine shrimp. On top of the losses due to diseases, it's important to mention that some of the vibrio species are zoonotic agents, meaning they can also cause diseases in humans. Given the importance of bacteria of the genus Vibrio to shrimp farming and public health, the aim of this study was to evaluate marine shrimp, correlate the clinical findings with the presence of the vibrio bacteria and identify the isolated species in the water of the ponds of cultivated marine shrimp in Pernambuco. The samples were collected from four coastal farms and the laboratory analysis consisted of the research and identification of the vibrio bacteria in the hepatopancreas and in the water of the tanks, and identification of any alteration by examination of fresh samples. Thiosulphate citrate bile salt agar (TCBS) was used as the culture media and the selected colonies were later biochemically tested. Four hundred and eighty shrimps were analyzed, of hose 424 (88.33%) showed to have vibrio and one or more alterations. There were identified a total of 903 alterations, the most frequent of muscle tissue (329; 36.43%) and the least frequent of the hepatopancreas tubules (121; 13.40%). When analizing the growth cycle, it was observed the largest number of alterations (503; 55.7%) in the dry season. Taking into account the weight of the shrimps, it was observed 540 (59.8%) alterations when the average weight was 8g. Out of all the analyzed animals, 314 (65.42%) showed to have vibrio, being 38 samples from the ponds water and 605 shrimp isolated. The most abundant species was V. mediterranei, (21.31%), considered an environmental species non pathogenic to shrimp, and the least abundant species were V. campbellii, V. rotiferanus, V. shiloni, V. splendidus, V. tapetis and V. wodanis (0.16 %). A dependency between the alterations found on the exam and the presence of the vibrio was noticed, showing that the aforementioned exam, combined to microbiological analyzes, is an important tool that should be used when monitoring the farms. It was also noticed a great variety of pathogenic and non pathogenic species of vibrio in the studied samples. Such microorganisms could, eventually, be related to shrimp and human infections, which could represent a risk for shrimp farming and public health respectively. A carcinicultura mundial tem enfrentado nos últimos anos vários impactos causados por enfermidades, que contribuíram para a queda dos índices de desenvolvimento na produção de camarão marinho cultivado. Além das perdas causadas devido às enfermidades, ressalta-se que algumas espécies de víbrio são agentes zoonóticos, ou seja, podem causar doenças também em humanos. Dada a importância das bactérias do gênero Vibrio para a carcinicultura e saúde pública, objetivou-se avaliar camarões marinhos, associar os achados clínicos com a presença de bactérias do gênero Vibrio e identificar as espécies isoladas da água dos viveiros e de camarões marinhos (Litopenaeus vannamei) cultivados no litoral do estado de Pernambuco. As amostras foram coletadas em quatro carciniculturas do litoral e as análises laboratoriais compreenderam pesquisa e identificação de bactérias do gênero Víbrio no hepatopâncreas e na água dos viveiros, e identificação de alterações através do exame a fresco. Para o isolamento das espécies foi utilizado o meio de cultivo Ágar Tiossulfato Citrato Sais de Bile Sacarose (TCBS) e as colônias selecionadas foram posteriormente submetidas a provas bioquímicas. Foram analisados no total 480 camarões, destes 424 (88,33%) apresentaram um ou mais tipos de alteração. Foi identificado um total de 903 alterações, sendo a mais freqüente a da musculatura (329; 36,43%) e a menos freqüente a alteração dos túbulos do hepatopâncreas (121; 13,40%). Com relação ao ciclo de cultivo, foi observado o maior número de alterações (503; 55,7%) no período de estio. Levando-se em consideração a média de peso dos camarões verificou-se 540 (59,8%) alterações quando os mesmos estavam com peso médio de 8 g. Do total de animais analisados 314 (65,42%) apresentavam vibrio e uma ou mais alteração no exame a fresco. Foi obtido um total de 643 isolados de Vibrio, sendo 38 de amostras de água dos viveiros e 605 isolados de camarão. A espécie mais abundante foi V. mediterranei (21,31%), considerada espécie ambiental e não patogênica para o camarão, e as espécies de menor predominância foram V. campbellii, V. rotiferianus, V. shiloni, V. splendidus, V. tapetis e V. wodanis (0,16%). Foi verificada uma dependência entre as alterações encontradas no exame a fresco e a presença de vibrio, concluindo-se que o referido exame, associado à análise microbiológica é uma importante ferramenta que deve ser utilizada no monitoramento da saúde dos animais. Verificou-se também uma ampla variedade de espécies patogênicas e não patogênicas de víbrio nas amostras estudadas, que podem eventualmente estar relacionados a infecções nos camarões e nos humanos, representando um risco para a carcinicultura e para a saúde pública respectivamente.

Submitted by (edna.saturno@ufrpe.br) on 2016-08-01T15:49:41Z No. of bitstreams: 1 Andrea Barretto.pdf: 465481 bytes, checksum: 80d81c9df4236400373841c9117f86d9 (MD5) Made available in DSpace on 2016-08-01T15:49:41Z (GMT). No. of bitstreams: 1 Andrea Barretto.pdf: 465481 bytes, checksum: 80d81c9df4236400373841c9117f86d9 (MD5) Previous issue date: 2009-02-02 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The study of blood cells, also known as hematology, has become for aquaculture a valuable tool in understanding the physiological changes that occur in organisms, however the shrimp, there is still much to discover about if the hemolymph. Considering the above objective of this work: to evaluate the effectiveness of the anticoagulant sodium citrate (10%) in blood tests, to check the influence of cooling on the total count of hemocytes (CTH), haematological data correlate with the presence of Vibrio in the hemolymph, to identify the influence of age and seasonality in the total count of haemocytes in shrimp Litopenaeus vannamei. The species were caught shrimp Litopenaeus vannamei from two nurseries of land (A and B) of mariculture Santa Cruz, located in the city of Goiana-PE. The collections are made weekly, for two cycles of cultivation (dry season and rainy season). It is the examination fresh (macro and microscopic) in all animals sampled. The total counts of hemocytes (cells/mm3) were performed immediately after collection and after five hours of chilling in a Neubauer chamber. Immediately after the counting, the samples intended for bacteriological analysis were transported in temperature to the laboratory of health of aquatic animals (LASAq), Universidade Federal Rural de Pernambuco. The data concerning the influence of cooling on the hemocytes were analyzed using the techniques of mathematical modeling (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-16T15:07:14Z No. of bitstreams: 1 Mauricio Nogueira da Cruz Pessoa.pdf: 716355 bytes, checksum: 41120b87fa94d8d978b32032793ede5f (MD5) Made available in DSpace on 2017-02-16T15:07:14Z (GMT). No. of bitstreams: 1 Mauricio Nogueira da Cruz Pessoa.pdf: 716355 bytes, checksum: 41120b87fa94d8d978b32032793ede5f (MD5) Previous issue date: 2008-10-01 The statistical evaluation of the use of extruded and pelleted ration in the Litopenaeus vannamei shrimp culture, under field conditions, were done with the objective of determinie the advantages of these diets on shrimp performance index. To perform the analyses were used 481 crops, from 8 commercial farms of northeastern Brazil. It was the technique used multiple linear regression to determine the parameters of handling variables that influenced the responses of crops such as production, productivity, the conversion factor of food (FCA), the weekly average growth, the average weight end, the quantity of feed consumed and survival. These responses were measured by the density of storage, but also in time of cultivation. It may be noted that there was no significant difference (P ≥ 0,05) between the use of feed on the variables average weight final and average weekly growth, as in other variables can be observed that for the storage density of 15 shrimps.m-2 there were differences of up to 20.1% in the FCA in favour of extruded ration, a production 9.7% higher when using pelleted ration of feed consumption and a 33.2% higher in pelleted feed. For densities of 60 shrimps.m-2 the difference in the FCA was 18.8% lower when you used to feed pelleted, production was 7.2% higher if using pelleted diet, consumption of feed was 8.5% lower when used if pelleted diet and productivity was 10.3% higher when the extruded feed was used for that density. Avaliações estatísticas do uso de ração extrusada e peletizada, em cultivos do camarão Litopenaeus vannamei, nas condições de campo, foram realizadas com o objetivo de determinar as vantagens sobre os índices zootécnicos dos camarões. Análises foram feitas utilizando-se 481 cultivos, provenientes de oito fazendas comerciais do nordeste do Brasil. Foi utilizada a técnica de regressão linear múltipla para estimar os parâmetros de manejo que influenciaram nas variáveis respostas dos cultivos tais como produção, produtividade, fator de conversão alimentar (FCA), crescimento semanal, peso final, quantidade de ração administrada e sobrevivência. Essas variáveis foram avaliadas em função da densidade de estocagem e do tempo de cultivo. Não houve diferença significativa (P ≥ 0,05) entre o uso das rações sobre as variáveis, peso final e crescimento semanal. Nas demais variáveis observou-se que na densidade de estocagem de 15 camarões/m2 houve diferença de até 20,1% no FCA a favor da ração extrusada, uma produção 9,7% maior quando se utilizou ração peletizada e um consumo a mais de 33,2% de ração peletizada. Para densidades de 60 camarões/m2 a diferença no FCA foi de 18,8% menor quando se utilizou ração peletizada, a produção foi 7,2% maior utilizando-se ração peletizada, o consumo de ração foi 8,5% menor com ração peletizada e a produtividade foi 10,3% maior quando a ração extrusada foi utilizada.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-21T16:48:08Z No. of bitstreams: 1 Samia Regia Rocha Monteiro.pdf: 361943 bytes, checksum: 282e86342e0a7dc7cefcb1a57d78fd60 (MD5) Made available in DSpace on 2017-02-21T16:48:08Z (GMT). No. of bitstreams: 1 Samia Regia Rocha Monteiro.pdf: 361943 bytes, checksum: 282e86342e0a7dc7cefcb1a57d78fd60 (MD5) Previous issue date: 2008-08-29 The use of artificial substrates in aquaculture has been used to provide the increase of culture area and natural food, improving the water quality of shrimp performance. In order to maximize the survival, feed conversion and final weight of marine shrimp Litopenaeus vannamei, cultured in oligohaline water (>0.5‰) and in heterotrophic system, were evaluated the effect of adding screens as artificial substrate. Post-larvae with 10 days (PL10) were acclimated from 34‰ to 0.5‰, during 12 days. After of the acclimatization process, the postlarvae (PL22) were reared to obtain 0.3±0.1g of weight and then they were transferred to 18 fiberglass tanks (800 L of net volume) and stocked with 150 PL/tanque. The substrate percentage addition (SPA), represents 100, 150, 200, 250 and 300% of bottom area (1m2) and the control “0” SPA, it was evaluated in completely randomized design, with three replicates. During the culture, were used a 40% crude protein (CP) ration during the first 30 days, andanother with 35% CP during the a 15 days, offered daily at 8, 12 and 17 hrs. After 45 days of culture the survival ranged from 45 to 84%, between treatments with 300% and 100% of substrate, respectively. The shrimp final weight, when related to the length orbit and the time of culture, did not present statistical difference between the SPA (P>0.05). In the ratio length and culture time, the treatment 300% was statistical different in the SPA-0, 200 and 250%. In conclusion, the use of artificial substrate increased the variables weight gain, feed conversion ratio, growth rate and survival when using SPA between 87.8 and 166.7%. O uso de substratos artificiais na aqüicultura vem sendo utilizado para disponibilizar maior área de cultivo e alimento natural, melhorando a qualidade de água e desempenho do camarão. Objetivando maximizar a sobrevivência, conversão alimentar e peso final do camarão marinho Litopenaeus vannamei, quando cultivado em água oligohalina (>0,5‰) e em meio heterotrófico, foram avaliadas a influência da adição de telas como substrato artificial. Pós-larvas com 10 dias (PL10) foram aclimatadas de 34‰ para 0,5‰, durante um período de 12 dias. Ao final do processo de aclimatação, as pós-larvas (PL22) foram cultivadas até se obter o peso de 0,3+0,1g e, posteriormente, foram transferidas para 18 tanques de fibra de vidro (800L de volume útil) e estocadas com 150 PL/tanque. A porcentagem de adição de substrato (PAS) foi representada por 100, 150, 200, 250 e 300% da área de fundo dos tanques (1,0 m2), e o controle com “0” de PAS, analisadas em delineamento inteiramente casualizado, com três repetições. Durante a fase de cultivo, foi utilizada, nos 30 primeiros dias, ração com40% de proteína bruta (PB) e nos 15 posteriores, com 35% PB, administrada diariamente às 8, 12 e 17 horas. Ao final de 45 dias de cultivo, a sobrevivência variou de 45 a 84%, entre os tratamentos com 300% e 100% de substrato, respectivamente. O peso final dos camarões, quando relacionado ao comprimento orbital e ao tempo de cultivo, não teve diferença estatística entre as PAS (P>0,05). Na relação entre comprimento com o tempo de cultivo, as PAS de 0, 200 e 250% diferenciaram de 300%. Conclui-se que o uso de substrato artificial favorece as variáveis ganho de peso, fator de conversão alimentar, taxa de crescimento diária e sobrevivência quando se utiliza PAS entre 87,8 e 166,7%.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-18T11:49:13Z No. of bitstreams: 1 Lilian Maria Nery de Barros Goes.pdf: 490274 bytes, checksum: f5fc8ea054d8a75de4f16f6764893075 (MD5) Made available in DSpace on 2016-10-18T11:49:13Z (GMT). No. of bitstreams: 1 Lilian Maria Nery de Barros Goes.pdf: 490274 bytes, checksum: f5fc8ea054d8a75de4f16f6764893075 (MD5) Previous issue date: 2008-07-02 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The blood cells normal values knowledge is one of the most valuable clinical labs technique which allows the systemic and morphologic anomaly, when identification is not cleary shown in the organism, identification. At the mammals medicine these values are great known, but on the shrimp culture there still be too much to clear up about haemolynph. Several studies have been realized aiming determines the different kinds of cells and their respective functions. However, the important information like the normal values of the hemocytes total counting (HTC) in different development phases to the Litopenaeus vannamei species and the existence of hemocytes behavior variations on males and females, as well as its relation to anatomo-histopatologic alterations, still being unknown. In face of the exposed, it was aimed to verify the hemocytes behavior on the different cultivated L. vannamei shrimp development phases, the sexual quantitative variation existence, the sodium citrate and Ethylenediaminetetraacetic acid (EDTA) efficacy as anticoagulant, beyond its relation to the detected alterations at the fresh and histopathology exams. 20 samples of haemolynph were collected weekly, being ten (five males and five females) using the sodium citrate anticoagulant and ten using the EDTA anticoagulant. The collects were realized in two aquariums (A and B), on summer and winter times at a commercial shrimp culture located in Goiana/Pernambuco estate. 0,1mL of haemolynph on a proportion of 1:1 (anticoagulant with 10%concentration:haemolynph) were collected from each shrimp to clinical pathology analyses. The hemocyties/mm3counting was realized immediately after the collect and after a five hours interval. The results were statistically analyzed using multiple linear moulds (P

Submitted by (ana.araujo@ufrpe.br) on 2016-07-06T16:01:03Z No. of bitstreams: 1 Iran Rodrigues de Oliveira.pdf: 478669 bytes, checksum: d4813a6f79bb512c0c9744fe30e4adef (MD5) Made available in DSpace on 2016-07-06T16:01:03Z (GMT). No. of bitstreams: 1 Iran Rodrigues de Oliveira.pdf: 478669 bytes, checksum: d4813a6f79bb512c0c9744fe30e4adef (MD5) Previous issue date: 2008-04-29 In this work, Data Envelopment Analysis was used to evaluate efficiency of marine shrimp Litopenaeus vannamei culture winth freshwater systems, considering relation between output variables (production and final mean weight) and input variables (culture production time, post larvae quantity, pond area, ration quantity), offered (pH, water transparency, ration brand and post larva e hatchery gounce). The database used for this study belongs to a commercial farm at Rio Grande do Norte – Brasil, composed of 115 observations collected from August 2008 to October 2005. Application of DEA indicates that 24 cultures were efficient, while 91 were not efficienty (efficiency less then). For each of the inefficient cultures individual efficiency optimization goals were established. It was also found that among the exogenous variables only temperature was related with the production efficiency in terms of final mean weignt shrimp, while dissolved oxygen pH, water transparency, ration brand and post larvae source do not demonstrate significant relevance (p ≥ 0,05). Para avaliar a eficiência do cultivo do camarão marinho Litopenaeus vannamei, em sistema dulciaqüicola, utilizou-se a técnica da Análise Envoltória dos Dados (Data Envelopment Analysis - DEA), considerando as variáveis de saída (produtos) produção e peso médio final quando associadas às de entrada (insumos) tempo de cultivo, quantidade de pós-larva, área do viveiro, quantidade de ração ofertada e as variáveis exógenas, marca de ração e laboratório de origem das póslarvas. Utilizou-se um banco de dados de uma fazenda comercial do Rio Grande do Norte – Brasil, composto por 115 observações, o qual foi gerado durante o período de Agosto de 2002 a Outubro de 2005, sendo utilizado o sistema bifásico (fase berçário e fase engorda). Com a técnica DEA evidenciaram-se 24 cultivos eficientes e 91 ineficientes (eficiência

Submitted by (edna.saturno@ufrpe.br) on 2016-08-11T16:51:11Z No. of bitstreams: 1 Carlos Andre Bezerra Alves.pdf: 107646 bytes, checksum: c97f6b2e6468643c3953a5613da56559 (MD5) Made available in DSpace on 2016-08-11T16:51:11Z (GMT). No. of bitstreams: 1 Carlos Andre Bezerra Alves.pdf: 107646 bytes, checksum: c97f6b2e6468643c3953a5613da56559 (MD5) Previous issue date: 2007-03-22 Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq Diseases means any adverse alteration in the health or acting in the productivity of the animals. In shrimps, the diseases can be unchained when it occur an unbalance potentially among the environmental conditions of nurseries, the health condition of the cultivated shrimps and the pathogenic agents, associated with the employed handling. This way, it was aimed at to evaluate the sanitary conditions and to determine which the relevant factors in the attack of the vibrioses in shrimp cultivated Litopenaeus vannamei. In the period of December of 2005 to November of 2006, were collected and analyzed shrimp samples, water and nursery soil, as well as technical information on the handling adopted in a commercial shrimp culture located in the north of Pernambuco/ Brazil. The handling parameters were correlated through mathematical models (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-22T12:37:59Z No. of bitstreams: 1 Suely Santos Bezerra.pdf: 418523 bytes, checksum: 6cbf43e2cf1c398824dd661f7ea8c038 (MD5) Made available in DSpace on 2017-02-22T12:37:59Z (GMT). No. of bitstreams: 1 Suely Santos Bezerra.pdf: 418523 bytes, checksum: 6cbf43e2cf1c398824dd661f7ea8c038 (MD5) Previous issue date: 2007-02-23 It can be observed nowadays that the increase in the commercial shrimp produc-tion occurs concomitantly with an enhancement of the incidence of diseases. These last ones can cause innumerable damages to the shrimp farmers in the whole world. Amongst these diseases, it can be distinguished the Infectious Hypodermal and Hema-topoietic Necrosis (IHHN), with viral etiology, that can be characterized by not causing great mortality, but body deformities. In addition to this, it frequently leads shrimp to heterogenic growth. For viruses diagnosis histology is an efficient tool, allowing the verification of the health of these crustaceans. This study aimed the observation of the occurrence of IHHN characteristic histopatologic injuries in Litopenaeus vannamei, and its relation with parameters of culture of marine shrimp farms in the state of Per-nambuco. After multiple regression statistic analysis it could be concluded that the tem-perature of the water of the pound and its oxygen availability are narrowly related with the occurrence of those histopatologic injuries. Ao mesmo tempo em que crescem os cultivos comerciais de camarão, observa-se o aumento da incidência de enfermidades que podem causar inúmeros prejuízos aos carcinicultores em todo o mundo. Dentre essas enfermidades, destaca-se a Infecção Hi-podermal e Necrose Hematopoiética (IHHN), de etiologia viral que, embora não cause grandes taxas de mortalidade, produz retardo no desenvolvimento e deformidades nos camarões. Para o diagnóstico dessa virose a histologia tem sido uma ferramenta bastante eficiente e que permite maior monitoramento da sanidade desses crustáceos. Desta for-ma, objetivou-se estudar a IHHN em Litopenaeus vannamei relacionando lesões histo-patológicas características e sua relação com variáveis de cultivo. Com base em regres-sões múltiplas conclui-se que existe uma alta relação entre as variáveis de cultivo tem-peratura e oxigênio e a ocorrência destas lesões.

Submitted by (edna.saturno@ufrpe.br) on 2017-02-21T16:11:44Z No. of bitstreams: 1 Roseli Pimentel Pinheiro e Silva.pdf: 245935 bytes, checksum: 769eb187840e50bd513ec06abe7683e0 (MD5) Made available in DSpace on 2017-02-21T16:11:44Z (GMT). No. of bitstreams: 1 Roseli Pimentel Pinheiro e Silva.pdf: 245935 bytes, checksum: 769eb187840e50bd513ec06abe7683e0 (MD5) Previous issue date: 2007-02-22 Considering the illnesses are barriers for the development of the carcinicultura, the vibriose has the possibility to occur for having as etiologic agent bacteria autochtonous of marine environment, it was objectified identification of the interferentes factors in the frequency of this disease. Four situated farms in the south coast of Pernambuco had been selected of which samples of water and shrimp during all had been collected monthly the phases of culture in two different climatic cycles (wet and rainy). Esteem the load of Vibrio spp., samples of water, post larvae had been analyzed, hemolymph and hepatopancreas and the countings had been correlated, through the use of mathematical models (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-07T15:03:38Z No. of bitstreams: 1 Anderson Goncalves Croccia.pdf: 343718 bytes, checksum: ab4281348333734382726c65db799fc0 (MD5) Made available in DSpace on 2017-02-07T15:03:38Z (GMT). No. of bitstreams: 1 Anderson Goncalves Croccia.pdf: 343718 bytes, checksum: ab4281348333734382726c65db799fc0 (MD5) Previous issue date: 2006-04-26 Abrupt changes of pH was conducted to estimate yhe influence on survive of the post-larvae of the srhimp Litopenaeus vannamei, in the 0‰, 10‰, 20‰ and 25‰ salinity. The post-larvaewas estocked of 50 post-larvae/L in a 18 plastic containers with the capacity of 2,5 liters. The pH water was 8,0 in the larviculture. Increase or decrease the pH water in 0,5, 1,0, 1,5, and 2,0 were used the calcium or acid acetic, respectivally. After 48 hours the post-larvae were counted one by one, to obtain the survaive rate. According to a mathematic model applied, it was shown the salinity wasn’t influencied significative (≥0,05) in survive of the post-larvae. Changes of pH to valoes more alcalinity were more impacty in the survive and to 8,0 to 10,0 made 100% of the mortality on the post-larvae. Mudanças bruscas do pH da água foram realizadas, para avaliar sua influência na sobrevivência de pós-larvas do camarão Litopenaeus vannamei, cultivadas em diferentes salinidades 0‰;10‰; 20‰ e 25‰.As pós-larvas foram estocadas a densidade de 50 pós-larvas/L em reservatórios plásticos com capacidade de 2,5 L. O pH inicial da água foi de 8,0, equivalente ao da larvicultura. Para aumentar ou diminuir o pH da água em 0,5, 1,0, 1,5 e 2,0, foram utilizados cal hidratada e ácido acético, respectivamente. Após 48 horas das pós-larvas terem sido submetidas as mudanças de pH da água, estas foram contadas individualmente, para estimar a taxa de sobrevivência. De acordo com o modelo matemático utilizado para correlacionar essas variáveis, verificou-se que a salinidade não influenciou significativamente (P ≥ 0,05) na sobrevivência das pós-larvas. Mudanças de pH para valores mais alcalinos foram mais impactantes na sobrevivência e quando foi de 8,0 para 10, gerou 100% de mortalidade das pós-larvas.

Submitted by (ana.araujo@ufrpe.br) on 2016-05-19T13:15:06Z No. of bitstreams: 1 Ady Marinho Bezerra.pdf: 631885 bytes, checksum: cbc9f196ed10a1296f54624a959cc596 (MD5) Made available in DSpace on 2016-05-19T13:15:06Z (GMT). No. of bitstreams: 1 Ady Marinho Bezerra.pdf: 631885 bytes, checksum: cbc9f196ed10a1296f54624a959cc596 (MD5) Previous issue date: 2006-02-24 It was objectified to correlate of the variable-response (production,productivity, final weight and tax of survival), with the water physic (temperature in the deep one, temperature in the surface and water level) and the chemical (salinity, oxygen in the deep one, oxygen in the surface, pH and transparency of the water) and the involved variable and the adopted handling (amount of ration,period of winter, period of summer, days of culture, initial density, supplying laboratory of after-larva, area of the fishery, number of cycles, initial population,aeration and days of preparation) of the culture of the marine shrimp Litopenaeus vannamei. The data base of the shrimp farm was composed for 68 comments, correspondents to the period of 2003 the 2005. Esteem the parameters of themodels it was used technique of the square minimums. The election of variable was carried through using the process of Stepwise Backward, associated the Box and Cox, to minimize the sum of square errors. The adequacy of the esteem equations and the hypothesis of linearity, normality and homogeneous variance for the errors had been analyzed on the basis of the analysis of variance and in the analysis of residue. According to the results, it was possible to conclude that the water physic and chemical influenced (p < 0.05) the variableness of the variableresponse Objetivou-se correlacionar as variáveis de cultivo (produção, produtividade,peso final e taxa de sobrevivência) com as variáveis físicas (temperatura no fundo do viveiro, temperatura na superfície do viveiro e nível da água do viveiro) e químicas da qualidade da água (salinidade da água, oxigênio no fundo do viveiro,oxigênio na superfície do viveiro, pH da água e transparência da água) e as variáveis envolvidas no manejo adotado (quantidade de ração, período de inverno, período de verão, dias de cultivo, densidade inicial, laboratório fornecedor de pós-larva, área do viveiro, número de ciclos, população inicial, aeração e dias de preparação) do camarão marinho Litopenaeus vannamei, e selecionar as variáveis que mais influenciaram em cada uma das variáveis respostas. O banco de dados da fazenda de camarão foi composto por 68 observações,correspondentes ao período de 2003 a 2005. Para estimar os parâmetros dos modelos se utilizou a técnica dos mínimos quadrados. A seleção de variáveis foi realizada utilizando-se o processo de Stepwise Backward, associado a Box e Cox, para minimizar a soma dos quadrados dos erros. A adequação das equações estimadas e os pressupostos de linearidade, normalidade e homocedasticidade para os erros foram analisados com base na análise de variância e na análise de resíduo. De acordo com os resultados, pôde-se concluir que as variáveis físicas e químicas da água tiveram grande influência (p < 0,05) na variabilidade das variáveis respostas

Submitted by (ana.araujo@ufrpe.br) on 2016-08-08T15:40:43Z No. of bitstreams: 1 Nedson Pereira Ximenes.pdf: 827888 bytes, checksum: 749ce307068c8493510b5eafaa124da5 (MD5) Made available in DSpace on 2016-08-08T15:40:43Z (GMT). No. of bitstreams: 1 Nedson Pereira Ximenes.pdf: 827888 bytes, checksum: 749ce307068c8493510b5eafaa124da5 (MD5) Previous issue date: 2005-08-08 The objective of the present work was to optimize the cultivation parameters of the marine shrimp (Litopenaeus vannamei, Bone, 1931) with the use of regression analyss . Cultivation data were used from a marine shrimp farm located in the north forest zone in the state of Pernambuco corresponding to the period from 2001 to 2003. The adopted cultivation system was a two-phased system where the first phase was accomplished in birth tanks and the second phase in fatting nurseries . The farm has two tanks with capacity of 60 m3 and five nurseries with areas that vary from 3.23 to 4.74 ha. During these periods, post –larva e from several origins were used: Tecmarão (RN), Aqualider (PE), Equabras , Verde-Mar, Netuno (PE) Compescal (RN). The post-larvae were storage in birth tanks with densities varied from 19 to 34 PL / L within a period that varied from 10 to 26 days. After this period the post-larvae were transferred to the fatting nurseries. From the total amount of 31 cultivations sets accomplished, the following variables were considered. Period of the year, cultivation time, storage density, nursery area, fatting time, number of cycles, origin of the post - larvae. and the responses; survival , final weight, the number of shrimps alive , biomass, factor of conversion feeding. It was used the multiple regression analyses to correlate these variables. and it was also used Box and Cox to select the best value of in which maximized the value of the statistical R2.. The consistency of the models were checked based on the stastistical F, R2 and residual analyses. We can conclude that the survival rate of the post-larvas of L.vanamme were bigger when the cultivation time was increased in the birth tanks phase and decreased in the fatting nurseries, with the increasing fatting density. The final weight of the shrimps decreased with the fatting time being of 11 grams for a cultivation period of 85 days. Objetivou-se, com o presente trabalho, otimizar os parâmetros de cultivo do Litopenaeus vanammei (Boone 1931), com o uso de analise de regressão linear múltipla. Utilizaram-se dados de cultivos de uma fazenda Comercial do camarão marinho, localizada na Zona da Mata Norte do Estado de Pernambuco, correspondente ao período de 2001 a 2003. O sistema de cultivo adotado foi bifásico, em que a primeira fase foi realizada em tanques berçário e a segunda em viveiros de engorda. A fazenda dispõe de dois tanques berçário com capacidade de 60m3 e cinco viveiros com área que variam de 3,23 a 4,74 ha. Durante esse período foram utilizadas pós-larvas de diversas procedências: Tecmarã (RN), Aqualider (PE), Equabras, Verde-mar, Netuno (PE) e Compescal (RN). As pós-larvas foram estocadas nos tanques berçário com densidades que variaram de 19 a 34 PL/L, durante 10 a 26 dias. Após esse período foram transferidas para os viveiros de engorda. De um total de 31 cultivo foram observadas as seguintes variáveis preditoras: Época do Ano, Tempo de Cultivo, Densidade de Estocagem, Àrea de Viveiro, Tempo de Engorda, Densidade de Engorda, Números de Ciclos e Origem das Pós-larvas, e as respostas: Sobrevivência, Peso Final, Número de Camarões Vivos, Biomassa e Fator de Conversão Alimentar. Foi utilizado o processo de Stepwise Forward para seleção de variáveis significativas (p < 0,05) e o de Box e Cox para minimizar a soma dos quadrados dos resíduos das equações. As consistências das equações foram avaliadas com base nas estatísticas F de Snedecor, R2 e na Análise de Resíduo. Na fase berçário pode-se modelar a taxa de sobrevivência com os parâmetros de cultivo. Na engorda, apenas a taxa de Sobrevivência, Biomassa e Fator de Conversão Alimentar foram correlacionados (P < 0,05).

Submitted by (edna.saturno@ufrpe.br) on 2017-02-16T13:41:53Z No. of bitstreams: 1 Maria Luciene Luzia Tavares Albuquerque.pdf: 748109 bytes, checksum: ed6d14d8d94e3639f3bb58a2eb31663a (MD5) Made available in DSpace on 2017-02-16T13:41:53Z (GMT). No. of bitstreams: 1 Maria Luciene Luzia Tavares Albuquerque.pdf: 748109 bytes, checksum: ed6d14d8d94e3639f3bb58a2eb31663a (MD5) Previous issue date: 2005-03-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES Experiments had been carried in the Laboratory of Shrimp culture of the Fishery Department of the Federal Rural University of Pernambuco (UFRPE), the purpose to acclimatize post larvae of the marine shrimp Litopenaeus vannamei to freshwater, during the period of 2003 at 2004. The experiments had been carried through in three phases, using post larvae with nine and ten days (PL9-10) and post larvae with twenty five and twenty six days (PL25-26) produced by commercial hatcheries. The acclimatization had been developed in aquariums with capacity of 44 liters each where the post larvae had stocked a density 11,3 PL/L, of with an acclimatization average time of of ten days.In first experiment had been tested concentrations lime, associates with the renewal rate daily of water of aquariums. In second experiment had been tested the concentration of lime and diets with two levels of artemia and artificial feed for shrimp. At the third experiment five diets with levels different of artemia, artificial feed for fish and artificial feed for shrimp had been tested. In the end of acclimatization in three experiments, post larvae had removed of the aquarium, counted, measured and weighted to calculate the survival, final weight, weight gain, final length and length gain. At first experiment the renewal rate of 50% daily of water de 50% were more efficient at post larvae parameter growth. In second experiment, diet D1 provided best survival than diet D2 (P < 0.05). While in third experiment, the survival rates were best to make useful artificial feed for fish. As conclusion, the water ofrenewal rate and diets had influenced significantly (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-15T15:13:45Z No. of bitstreams: 1 Lilian Maria Nery de Barros Goes.pdf: 1347795 bytes, checksum: 24168cc057093f00ff74643a016d16ee (MD5) Made available in DSpace on 2017-02-15T15:13:45Z (GMT). No. of bitstreams: 1 Lilian Maria Nery de Barros Goes.pdf: 1347795 bytes, checksum: 24168cc057093f00ff74643a016d16ee (MD5) Previous issue date: 2005-02-15 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES In global aquaculture, shrimp farming has stood out as a highly profitable activity, contributing to the economic growth of agribusiness. In Brazil, the activity performs well, mobilizing large investments and generating jobs mainly in the Northeast, whose production is largely destined for exports. In this context, the quality of the product assumes fundamental importance, being necessary the use of additives. Sodium metabisulfite is the most widely used preservative in shrimp farming, acting as a molecular oxygen inhibiting agent, preventing the melanose reaction. In this way, the objective was to contribute to the reduction of the residual content of this preservative in cultivated prawns destined to the external and internal market. Shrimp of the Litopenaeus vannamei classification 81/100 were submitted to ten concentrations of sodium metabisulphite (from 1 to 10%) during five exposure times (10, 15, 20, 25 and 30 min) in order to establish the relationship Between the preservative concentration and the immersion time of the shrimp. The different methods of measuring SO2 concentration in shrimps and the residual levels of SO2 in frozen shrimps were also evaluated during the 1-month period. The results were statistically analyzed using multiple linear models. Concentrations of metabisulfite, above 4%, exceeded the maximum limits permitted by current legislation for cold and frozen shrimp (100 ppm residual SO2) being inappropriate for practical use. The shrimp submitted to concentration 1% showed good conservation during the first 20 days, however after this period the onset of melanose was detected. Concentrations 2 and 3% adequately preserved the product for a period of 30 days, with satisfactory SO2 levels. Storage time did not significantly influence (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-15T16:38:09Z No. of bitstreams: 1 Luis Otavio Brito da Silva.pdf: 481384 bytes, checksum: efd3c9a144f09ac28f9c1fc4d43626b1 (MD5) Made available in DSpace on 2017-02-15T16:38:09Z (GMT). No. of bitstreams: 1 Luis Otavio Brito da Silva.pdf: 481384 bytes, checksum: efd3c9a144f09ac28f9c1fc4d43626b1 (MD5) Previous issue date: 2004-11-01 The fertilizers based on urea and ammonia as nitrogen source is more used in aquaculture, mainly for their low cost. However it should be pointed out that those fertilizers in high doses could cause ammonia toxicity. On the other hand, fertilizers based on nitrates, even with superior costs, present advantages over those based on ammonia, because nitrate is not toxic and it is totally oxidized in the cultivation atmosphere. It was aimed at with the present work to test the use of sodium nitrate in ponds of sea shrimp. It was considered two treatments: NS (sodium nitrate enriched with phosphate, silicate, boron, magnesium, sulfur and potassium) and SNS (urea, triple super phosphate and sodium silicate). Sampling and analyses of the water were accomplished, being weekly performed for the plankton and for the soil in the beginning and in the end of the cultivation cycle. The application of sodium nitrate was initially in the soil and later in the water. For statistical analysis it was used T test (P

Submitted by (edna.saturno@ufrpe.br) on 2017-02-16T12:31:52Z No. of bitstreams: 1 Marcelo Estima Seabra Magalhaes.pdf: 1178529 bytes, checksum: dea80e3fc907fcbbe8eccee0a9c004b5 (MD5) Made available in DSpace on 2017-02-16T12:31:52Z (GMT). No. of bitstreams: 1 Marcelo Estima Seabra Magalhaes.pdf: 1178529 bytes, checksum: dea80e3fc907fcbbe8eccee0a9c004b5 (MD5) Previous issue date: 2004-02-16 Marine shrimp in Brazil has grown very fast in the last years due to adoption of new technologies applied to the activity. One of these refers to the use of nursery tanks to post larvae acclimation from the hatcheries. These nursery tanks have the advantage to reduce the culture time, increasing the ponds rotativity and consequently increasing the production. So, this work was done in order to evaluate the productivity of a marine shrimp culture through a three phases model with the use of primary and secondary nursery tanks, and growout pond without aeration. The experiment was held at Boqueirão da Ilha Farm Ltd (Lat 7º45’16.5” South; Long. 34º52’20.6” West), located at Itamaracá island, 60 Km far from Recife-PE. It was utilized a primary nursery tank (TBP), two secondary nursery tanks (TBS) and a 7000m2 growout pond. In the culture it was utilized Litopenaeus vannamei post larvae (0.001g) in the first phase with culture time of 25-30 days, which were transferred to the second phase (0.15g) where remaining for more 30 days and finally, with 2.34 g, were transferred to the growout pond, where remained for more 50-60 days to reach to market size. The tanks and pond were previously preparedwith nitrate sodium and sodium enriched with phosphorus in a quantity of 11g/m2 and 8.6g/m2, respectively. In the first phase the shrimp feeding was done with a 40 and 35% crude protein pelleted commercial ration, and this last one was used to the following phases. In the primary nursery tank, the weight gain (WG), specific growth rate (SGR) and survival (S) were 4.73mg/day, 9.77%/day and 88.56%, respectively. In the second phase the WG, SGR and S were 71.67mg/day, 9.10 %/day and 63.34%, respectively. In growout pond the WG, SGR and S were 147mg/day, 3.12%/day and 66.21%, respectively, obtaining shrimps with 11g of average weight. This system provided a 50-60 days growout phase, obtaining shrimp with 10-12g, reaching five culture cycles per year and a productivity in about 6.735Kg/ha/yr. The utilization of the three phase’s systems represents a new technology in order to optimize the pond growout areas. A carcinicultura marinha no Brasil, tem mostrado extraordinários avanços nos últimos anos, devido à utilização de tecnologias inovadoras que vem sendo sucessivamente aplicadas à atividade. Uma dessas, diz respeito à utilização de tanques berçários para adaptação de pós-larvas oriundas dos laboratórios de larvicultura. Estes tanques berçários têm a vantagem de reduzir o tempo de cultivo, aumentando a rotatividade dos viveiros e conseqüentemente incrementando a produção. Sob este enfoque, o presente trabalho foi realizado com objetivo de avaliar a produtividade em um cultivo de camarões marinhos, através de um modelo trifásico, com uso de tanques berçários primário e secundários, e viveiro de engorda sem aeração. mediante duas repetições. O experimento foi realizado na Fazenda Boqueirão da Ilha Ltda (Lat. 7º45’16,5”Sul; Long. 34º52’20,6” Oeste), localizada na Ilha de Itamaracá, que dista 60km de Recife-PE. Foram utilizados um tanque berçário primário (TBP), dois tanques berçários secundários (TBS) e um viveiro de engorda (VE) de 7000m². No cultivo foi utilizado pós-larvas (PL19) com 0,007g de Litopenaeus vannamei, na primeira fase com duração de 25-30 dias, as quais foram transferidas para segunda fase (0,15g) onde passaram mais 30 dias, e finalmente com 2,34g passaram para a terceira fase, onde permaneceram por 50-60 dias, atéatingirem o tamanho comercial. Os ambientes de cultivo foram previamente fertilizados com Nitrato de Sódio puro e enriquecido com Fósforo, em quantidades de 11g/m² e 8,6g/m², respectivamente. Na primeira fase a alimentação dos camarões foi efetuada com ração comercial contendo 40 e 35% de proteína bruta, sendo esta última utilizada para as fases seguintes. No tanque berçário primário, o ganho de peso médio (GP), a taxa de crescimento específico (TCE) e a sobrevivência (S) foram de 4,73 mg/dia, 9,77%/dia e 88,56%, respectivamente. Na segunda fase, o GP, TCE e S foram 71,67mg/dia, 9,10%/dia e 63,34%, respectivamente. No viveiro de engorda, o GP, TCE e S foram 147mg/dia, 3,12%/dia e 66,21%, respectivamente, obtendo-se camarões com 11g de peso médio. Esse sistema proporcionou uma fase de engorda com 50-60 dias de permanência no viveiro, obtendo-se camarão com peso de 10-12 g, que possibilita a obtenção de 5 ciclos anuais, e uma produtividade estimada da ordem de 6.735kg/ha/ano. O sistema de cultivo trifásico pode representar uma nova tecnologia que busca otimizar o aproveitamento das áreas de engorda de camarões.