1. Expression of Genes and Proteins of the Sarcoplasmic Reticulum Са 2+ -Transport Systems in Cardiomyocytes in Concomitant Coronary Heart Disease and Type 2 Diabetes Mellitus.
- Author
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Afanas'ev SA, Kondrat'eva DS, Muslimova EF, Budnikova ОV, Akhmedov SD, and Kozlov BN
- Subjects
- Aged, Biological Transport genetics, Biopsy, Calcium metabolism, Calsequestrin genetics, Calsequestrin metabolism, Case-Control Studies, Gene Expression, Humans, Middle Aged, Myocardium metabolism, Myocytes, Cardiac pathology, Ryanodine Receptor Calcium Release Channel genetics, Ryanodine Receptor Calcium Release Channel metabolism, Sarcoplasmic Reticulum pathology, Sarcoplasmic Reticulum Calcium-Transporting ATPases genetics, Sarcoplasmic Reticulum Calcium-Transporting ATPases metabolism, Calcium Signaling genetics, Coronary Disease complications, Coronary Disease genetics, Coronary Disease metabolism, Coronary Disease pathology, Diabetes Mellitus, Type 2 complications, Diabetes Mellitus, Type 2 genetics, Diabetes Mellitus, Type 2 metabolism, Diabetes Mellitus, Type 2 pathology, Myocytes, Cardiac metabolism, Sarcoplasmic Reticulum metabolism
- Abstract
We compared the expression of Са
2+ -ATPase (SERCA2a), calsequestrin (CASQ2), ryanodine receptors (RyR2) proteins and their genes (ATP2A2, CASQ2, and RYR2) in coronary heart disease (CHD) patients with and without comorbid type 2 diabetes mellitus. All studies were performed on the right atrial appendages resected during coronary bypass surgeries. Expression of SERCA2a and RyR2 proteins and their ATP2A2 (p=0.046) and RYR2 genes in comorbid pathology was significantly (p=0.042) higher (by 1.2 and 2 times; p=0.025). The expression of CASQ2 protein and its gene did not differ significantly between the groups (p=0.82 and p=0.066, respectively). It was concluded that the expression of SERCA2a and RyR2 proteins and their genes (but not CASQ2 and its gene) is elevated in CHD associated with type 2 diabetes mellitus. Expression of the studied proteins correlated with the expression of their genes. Increased expression of CASQ2 protein and its gene can probably prevent imbalance of the Ca2+ -transporting systems in cardiomyocytes and contractile dysfunction of the myocardium, even in CHD associated with type 2 diabetes mellitus., (© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)- Published
- 2021
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