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The aim of this study was to investigate the relationship of m6A RNA methylation to CaOX-induced renal tubular injury. Microarray analysis was performed to detect the difference in mRNA expression and m6A methylation between the injurious groups and controls. We established injurious renal tubular epithelial cell model induced by calcium oxalate crystals (CaOX), and we validated that CaOX could increase the overall m6A methylation levels. By microarray analysis, we identified 5967 differentially expressed mRNAs (2444 were up-regulated and 3523 were down-regulated in the injurious groups) and 6853 differentially methylated mRNAs (4055 were in hypermethylation and 3688 were in hypomethylation in the injurious groups). Four clusters (hyper-up, hyper-down, hypo-up and hypo-down) were further identified via conjoint analysis. Functional analysis revealed that m6A methylation played a crucial role in the development of CaOX through participating multiple processes covering inflammation, oxidative stress, apoptosis, crystal-cell adhesion. We delineated the first transcriptome-wide m6A landscape of injurious renal tubular cells in high-CaOX environment. We identified a series of mRNAs of renal tubular epithelial cells with differential expression and m6A methylation between the CaOX-treated groups and controls., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The prevalence of kidney stones continues to increase worldwide, however, there are still short of effective drugs against urinary stones. In our study, we developed and utilized porous Se@SiO 2 nanospheres as the novel antioxidant agent to detect its potential effects on crystallization inhibition. Porous Se@SiO 2 nanospheres enable to deliver Se sustainably with lower cytotoxicity and preferred biocompatibility. In vivo, we found that porous Se@SiO 2 nanospheres markedly decreased CaOX crystal deposition in renal parenchyma and alleviated kidney oxidative damage. In vitro, we found that the antioxidant effects of porous Se@SiO 2 nanospheres were mediated by upregulation of HO-1 and activation of Keap1/Nrf2 pathway. These results suggests that porous Se@SiO 2 nanospheres may be a new promising therapeutic option for stone formers. [ABSTRACT FROM AUTHOR]