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Your search keyword '"Yeo Cho Yoon"' showing total 5 results
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5 results on '"Yeo Cho Yoon"'

1. Limonin, a Component of Dictamni Radicis Cortex, Inhibits Eugenol-Induced Calcium and cAMP Levels and PKA/CREB Signaling Pathway in Non-Neuronal 3T3-L1 Cells

Author

Yeo Cho Yoon, Sung-Hee Kim, Min Jung Kim, Hye Jeong Yang, Mee-Ra Rhyu, and Jae-Ho Park

Subjects
limonin, furanolactone, cAMP, olfactory, calcium, dictamni radices cortex, Organic chemistry, QD241-441
Abstract

Limonin, one of the major components in dictamni radicis cortex (DRC), has been shown to play various biological roles in cancer, inflammation, and obesity in many different cell types and tissues. Recently, the odorant-induced signal transduction pathway (OST) has gained attention not only because of its function in the perception of smell but also because of its numerous physiological functions in non-neuronal cells. However, little is known about the effects of limonin and DRC on the OST pathway in non-neuronal cells. We investigated odorant-stimulated increases in Ca2+ and cAMP, major second messengers in the OST pathway, in non-neuronal 3T3-L1 cells pretreated with limonin and ethanol extracts of DRC. Limonin and the extracts significantly decreased eugenol-induced Ca2+ and cAMP levels and upregulated phosphorylation of CREB and PKA. Our results demonstrated that limonin and DRC extract inhibit the OST pathway in non-neuronal cells by modulating Ca2+ and cAMP levels and phosphorylation of CREB.

Published
2015
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2. Piperine, a component of black pepper, decreases eugenol‐induced cAMP and calcium levels in non‐chemosensory 3T3‐L1 cells

Author

Sung Hee Kim, Mee-Ra Rhyu, Yeo Cho Yoon, Min Jung Kim, Hye Jeong Yang, and Jae-Ho Park

Subjects
QH301-705.5, chemistry.chemical_element, Pharmacology, Calcium, CREB, Article, General Biochemistry, Genetics and Molecular Biology, chemistry.chemical_compound, cAMP, Pepper, Medicine, Biology (General), 3T3-L1, PNF, Piper nigrum fructus, Black pepper, Traditional medicine, biology, business.industry, Piperine, Olfactory, Piper nigrum, Eugenol, OST, odorant-induced signal transduction, chemistry, biology.protein, CREB, cAMP response element-binding protein, Phosphorylation, Signal transduction, business
Abstract

Highlights • An ethanol extract of black pepper inhibits eugenol-induced signal transduction. • The extract reduces eugenol-induced cAMP and Ca2+ levels, and phosphorylation of CREB. • Piperine inhibits eugenol-induced signal transduction. • Piperine reduces eugenol-induced cAMP and Ca2+ levels, and phosphorylation of CREB., This study investigated the effects of an ethanol extract of black pepper and its constituent, piperine, on odorant-induced signal transduction in non-chemosensory cells. An ethanol extract of black pepper decreased eugenol-induced cAMP and calcium levels in preadipocyte 3T3-L1 cells with no toxicity. Phosphorylation of CREB (cAMP response element-binding protein) was down-regulated by the black pepper extract. The concentration (133.8 mg/g) and retention time (5.5 min) of piperine in the ethanol extract were quantified using UPLC–MS/MS. Pretreatment with piperine decreased eugenol-induced cAMP and calcium levels in 3T3-L1 cells. Piperine also decreased the phosphorylation of CREB, which is up-regulated by eugenol. These results suggest that piperine inhibits the eugenol-induced signal transduction pathway through modulation of cAMP and calcium levels and phosphorylation of CREB in non-chemosensory cells.

Published
2014
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3. Ethanol Extract of Polygonatumofficinale Rhizome Inhibits Odorant-Induced Camp and Calcium Levels in Non-Chemosensory 3T3-L1 Cells

Author

Mee Ra Rhyu, Jae-Ho Park, Myung-Sunny Kim, Jin-Taek Hwang, Sung Hee Kim, Yeo Cho Yoon, Haeng Jeon Hur, and Mi Jeong Sung

Subjects
Olfactory system, medicine.medical_specialty, biology, chemistry.chemical_element, 3T3-L1, Calcium, CREB, biology.organism_classification, Adenylyl cyclase, Polygonatum, chemistry.chemical_compound, Endocrinology, chemistry, Internal medicine, medicine, biology.protein, Phosphorylation, Signal transduction
Abstract

Polygonatum Officinalerhizome, a member of the liliaceae family, is commonly consumed as tea in Asia. It is also clinically used to treat obesity and fatigue in Korean traditional medicine. Although the anti-diabetic effect of POR has been described, little is known about its physiological role in the olfactory system. In this study, we investigated the effects of POR in 3T3-L1 cells expressing an odorant receptor. We have shown that the levels of cAMP and Ca2+ and the phosphorylation of Rap1A and CREB increased in response to an odorant, eugenol. POR significantly decreased the eugenol-induced increase in cAMP and Ca2+. Taken together, these data suggest that POR inhibits an odorant-induced signal transduction pathway.

Published
2014
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4. Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis

Author

Ae Sin Lee, Sung-Hee Kim, JaeHyung Koo, Mee-Ra Rhyu, NaNa Kang, Yeo Cho Yoon, and Jae-Ho Park

Subjects
Male, Angiogenesis, Biophysics, Neovascularization, Physiologic, Biology, Receptors, Odorant, Biochemistry, Umbilical vein, Mice, medicine.artery, medicine, Human Umbilical Vein Endothelial Cells, Animals, Humans, Phosphorylation, Extracellular Signal-Regulated MAP Kinases, Molecular Biology, Protein kinase B, Aorta, Gene knockdown, Olfactory receptor, Migration Assay, Cell Biology, Coronary Vessels, Cell biology, Mice, Inbred C57BL, medicine.anatomical_structure, Gene Knockdown Techniques, Immunology, cardiovascular system, Calcium, Endothelium, Vascular, Proto-Oncogene Proteins c-akt
Abstract

ORs are ectopically expressed in non-chemosensory tissues including muscle, kidney, and keratinocytes; however, their physiological roles are largely unknown. We found that human olfactory receptor 10J5 (OR10J5) is expressed in the human aorta, coronary artery, and umbilical vein endothelial cells (HUVEC). Lyral induces Ca 2+ and phosphorylation of AKT in HUVEC. A knockdown study showed the inhibition of the lyral-induced Ca 2+ and the phosphorylation AKT and implied that these processes are mediated by OR10J5. In addition, lyral enhanced migration of HUVEC, which were also inhibited by RNAi in a migration assay. In addition, matrigel plug assay showed that lyral enhanced angiogenesis in vivo . Together these data demonstrate the physiological role of OR10J5 in angiogenesis and represent roles of ORs in HUVEC cells.

Published
2015

5. GRAMINE SUPPRESSES EUGENOL-INDUCED CALCIUM AND CAMP ELEVATION IN NON-NEURONAL 3T3-L1 CELLS.

Author

Sung-Hee Kim, Yeo Cho Yoon, Jaewon Shim, Min Jung Kim, Dayu Jung, Jeongsoo Han, Mee-Ra Rhyu, and Jae-Ho Park

Subjects
*ALKALOIDS, *CALCIUM, *CELLULAR signal transduction, *CYCLIC adenylic acid, *NEURONS, *PHOSPHORYLATION, *PROTEIN kinases, *CARBOCYCLIC acids
Abstract

Gramine is an alkaloid found in giant reed and maple. Many reports discuss the health-beneficial effects of gramine in inflammation, angiogenesis, and cell growth. However, the physiological role of gramine in the odorant-induced signal transduction pathway has not yet been investigated. Therefore, in this study, we aimed to evaluate the effects of gramine on odorant-stimulated non-neuronal 3T3-L1 cells endogenously expressing the murine eugenol receptor, olfr73. We showed that gramine inhibited the eugenol-induced calcium influx and cyclic AMP (cAMP) elevation in the 3T3-L1 cells without any toxicity. In addition, gramine decreased the phosphorylation of protein kinase A and cAMP response element binding protein (CREB), which are known as downstream targets of cAMP, in eugenolstimulated 3T3-L1 cells. These results indicate that gramine suppresses the eugenol-induced signal transduction pathway in non-neuronal 3T3-L1 cells by modulating calcium influx, cAMP levels, and CREB phosphorylation. [ABSTRACT FROM AUTHOR]

Published
2018

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