1. Low-intensity pulsed ultrasound (LIPUS) stimulates mineralization of MC3T3-E1 cells through calcium and phosphate uptake.
- Author
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Tassinary JAF, Lunardelli A, Basso BS, Dias HB, Catarina AV, Stülp S, Haute GV, Martha BA, Melo DADS, Nunes FB, Donadio MVF, and Oliveira JR
- Subjects
- 3T3 Cells, Alkaline Phosphatase metabolism, Animals, Cell Differentiation, Cell Line, In Vitro Techniques, Mice, Transforming Growth Factor beta metabolism, Calcium metabolism, Collagen metabolism, Durapatite metabolism, Osteoblasts cytology, Osteoblasts metabolism, Phosphates metabolism, Ultrasonic Waves
- Abstract
The present study aimed to evaluate the effect of low-intensity pulsed ultrasound (LIPUS) on pre-osteoblast mineralization using in vitro bioassays. Pre-osteoblastic MC3T3-E1 cells were exposed to LIPUS at 1 MHz frequency, 0.2 W/cm
2 intensity and 20% duty cycle for 30 min. The analyses were carried out up to 336 h (14 days) after exposure. The concentration of collagen, phosphate, alkaline phosphatase, calcium and transforming growth factor beta 1 (TGF-β1) in cell supernatant and the presence of calcium deposits in the cells were analyzed. Our results showed that LIPUS promotes mineralized nodules formation. Collagen, phosphate, and calcium levels were decreased in cell supernatant at 192 h after LIPUS exposure. However, alkaline phosphatase and TGF-β1 concentrations remained unchanged. Therapeutic pulsed ultrasound is capable of stimulating differentiation and mineralization of pre-osteoblastic MC3T3-E1 cells by calcium and phosphate uptake with consequent hydroxyapatite formation., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2018
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