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The effect of phytase on phosphorus retention, broiler (Gallus gallus) performance and bone mineralization in diets with reduced inorganic phosphate concentration is well documented. Furthermore, so-called 'extra-phosphoric' effects of phytase have been described in the literature that may be associated with changes in mineral and amino acid partitioning and requirements per se. In particular, the role of myo-inositol in phytase responses is implied but not well elucidated. It was the purpose of the experiment reported herein to explore the effect of phytase on broiler growth, nutrient digestibility, blood biochemistry and gene expression. A 5-week broiler floor pen trial was conducted to evaluate the effect of supplementation of a moderately phosphorus-deficient diet with 1000 U/kg of a 6-microbial phytase. Parameters measured were growth performance, phosphorus (P), calcium (Ca) and myo-inositol plasma concentrations, apparent ileal P digestibility, bone mineralization, breast meat weight and Pectoralis major muscle transcriptome. Supplementation of the diet with phytase improved weight gain during the starter period (18%) and the whole period (24%) compared with animals that received the control diet (p

The effect of high (1000–3000 phytase units (FYT)/kg) doses of microbial phytase on performance, nutrient digestibility and plasma inositol concentrations in young Ross broiler chicks was investigated in two separate experiments. In both experiments pelleted corn/soy-based diets were used and experimental duration was from Days 8 to 21 and Days 15 to 28 in Experiments 1 and 2, respectively. Treatments in Experiment 1 were arranged as a 2 × 4 + 1 factorial with two concentrations of calcium and available phosphorus and four concentrations of phytase (0, 1000, 2000 or 3000 FYT/kg), with a reference diet containing additional phosphorus and calcium from inorganic sources. In Experiment 2 only four dietary treatments were used, being a nutritionally adequate positive control, a negative control formulated to be insufficient in calcium and available phosphorus and the negative control supplemented with either 1000 or 2000 FYT/kg exogenous phytase. In both experiments, phytase improved performance relative to the appropriate control diet and increased the retention of calcium and phosphorus (P < 0.001). Tibia strength and ash content were increased (P < 0.001) by phytase addition. Plasma inositol concentrations were substantially increased (P < 0.001) by phytase addition to the diet. As inositol has been found to be an insulin mimetic in a range of animal species, these results suggest that part of the beneficial effect of high doses of phytase in broiler production may be conferred via insulin-like mechanisms. The effect of phytase on the expression of insulin-sensitive glucose transport systems, gluconeogenesis and nitrogen cycling is an area for future research. It can be concluded that phytase is effective in improving performance of broiler chicks fed diets that are sufficient and insufficient in calcium and phosphorus. Furthermore, phytase addition results in increased plasma inositol concentrations that may be beneficial in nutrient transport and protein deposition.