1. An optical recording system based on a fast CCD sensor for biological imaging.
- Author
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Mammano F, Canepari M, Capello G, Ijaduola RB, Cunei A, Ying L, Fratnik F, and Colavita A
- Subjects
- Animals, Calcium metabolism, Cochlea metabolism, Electrophysiology, Fluorescent Dyes metabolism, Guinea Pigs, Hippocampus metabolism, Organic Chemicals, Rats, Time Factors, Calcium analysis, Microscopy, Video instrumentation, Microscopy, Video methods
- Abstract
This paper presents technical details, hardware and software of a complete imaging system which uses a fast CCD sensor and a 41 Msample/s A/D converter to acquire full-frame 12 bit/pixel digitized images with a time resolution of 1.25 ms/image. This apparatus permits to resolve intracellular Ca2+ gradients in individual cells as well as the spatio-temporal pattern of neural activity of cell assemblies in neural tissue.
- Published
- 1999
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