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Objective: The aim of this study was to explore the relationship between simple obesity and serum concentrations of essential elements potassium (K), sodium (Na), calcium (Ca), iron (Fe), copper (Cu), magnesium (Mg) and zinc (Zn) in rats. Methods: 20 male Wistar rats were randomly divided into two groups: the control group was given common forage, and the fat group was given high-fat forage. K, Na, Ca, Mg, Cu, Fe and Zn in serum and forage were determined by flame atomic absorption spectrometry. Results: During this study, there was no significant difference in forage consumption between control group and fat group (p > 0.05), and there was no difference in K, Ca, Mg, Cu, Fe, and Zn levels between common and fat forge (p > 0.05). The serum concentrations of Ca, Mg, Cu, Fe and Zn in control group (Ca 22.75 ± 2.166 mg/dL, Mg 3.452 ± 1.502 mg/ dL, Cu 2.490 ± 0.6493 mg/L, Fe 3.5910 ± 1.070 mg/L, Zn 2.146 ± 0.6069 mg/L) were obviously higher than that of fat group (Ca 11.05 ± 1.276 mg/dL, Mg 1.500 ± 0.3824 mg/dL, Cu 1.017 ± 0.4727 mg/L, Fe 1.765 ± 0.4120 mg/L, Zn 1.058 ± 0.2811 mg/L) (p < 0.05 for Mg, Fe, p < 0.01 for Ca, Cu, Zn). The lee's index of rats negatively correlated with the serum concentrations of Cu, Fe (p <0.01) and Ca, Zn (p < 0.05). Conclusions: The simple obesity could decrease the serum concentrations of Ca, Fe, Cu and Zn in rats. [ABSTRACT FROM AUTHOR]