1. D-pinitol ameliorated H 2 O 2 -induced oxidative damage in PC12 cells and prolonged the lifespan by IIS pathway in Caenorhabditis elegans.
- Author
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Zhang M, Xu Z, Shao L, Wang J, He Z, Jiang Y, Zhang Y, and Wang H
- Subjects
- Animals, Rats, Longevity, Hydrogen Peroxide toxicity, Hydrogen Peroxide metabolism, Antioxidants pharmacology, Antioxidants metabolism, Reactive Oxygen Species metabolism, PC12 Cells, Molecular Docking Simulation, Oxidative Stress, Transcription Factors metabolism, Superoxide Dismutase metabolism, Forkhead Transcription Factors metabolism, Caenorhabditis elegans, Caenorhabditis elegans Proteins genetics, Caenorhabditis elegans Proteins metabolism
- Abstract
D-pinitol (DP) has been extensively regarded as the main active component of legumes for anti-aging. In this study, we intended to explore the anti-aging mechanism of DP, utilizing computer modeling techniques. The results demonstrated that DP significantly delayed H
2 O2 -induced cellular senescence. Model PC12 cells treated with DP exhibited increased cell viability, increased antioxidant enzyme activity (SOD, CAT), and reduced ROS and MDA levels. Furthermore, DP was discovered to have a positive effect on healthy longevity. In C. elegans, DP treatment enhanced lifespan, stress capacity, antioxidant capacity (T-SOD/CAT/GSH-Px/MDA/ROS), and altered aging-related indicators of lipofuscin accumulation, pharyngeal pump rate, motility, and reproduction. Moreover, DP could reduce the toxicity Aβ in transgenic C. elegans CL4176, CL2355, and CL2331. Further mechanistic studies indicated DP increased transcription factor (daf-16, skn-1, hsf-1) expression of insulin/insulin-like growth factor-1 signaling (IIS) pathway. As expected, DP also extended the downstream target genes of the three transcription factors (sod-3, ctl-1, ctl-2, gst-4, hsp-16.1, and hsp-16.2). Further mutant lifespan experiments, network pharmacology, and molecular docking revealed that DP might be life-extending through the IIS pathway. DP deserves extensive investigation and development as a potential anti-aging drug in the future., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests., (Copyright © 2023. Published by Elsevier Inc.)- Published
- 2023
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