1. Short communication: In vivo screening platform for bacteriocins using Caenorhabditis elegans to control mastitis-causing pathogens.
- Author
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Son SJ, Park MR, Ryu SD, Maburutse BE, Oh NS, Park J, Oh S, and Kim Y
- Subjects
- Animals, Anti-Bacterial Agents pharmacology, Bacillus licheniformis metabolism, Cattle, Chloramphenicol pharmacology, Cost-Benefit Analysis, Female, Genes, Bacterial, RNA, Bacterial genetics, Sequence Analysis, RNA, Staphylococcal Infections drug therapy, Staphylococcal Infections veterinary, Staphylococcus aureus genetics, Staphylococcus aureus growth & development, Staphylococcus aureus isolation & purification, Bacteriocins pharmacology, Caenorhabditis elegans microbiology, Mastitis, Bovine drug therapy, Staphylococcus aureus drug effects
- Abstract
This study aimed to develop an in vivo screening platform using Caenorhabditis elegans to identify a novel bacteriocin for controlling the mastitis-causing pathogen Staphylococcus aureus strain RF122 in dairy cows. Using Bacillus spp. isolated from traditional Korean foods, we developed a direct in vivo screening platform that uses 96-well plates and fluorescence image analysis. We identified a novel bacteriocin produced by Bacillus licheniformis strain 146 (lichenicin 146) with a high in vivo antimicrobial activity using our liquid C. elegans-Staph. aureus assay. We also determined the characteristics of lichenicin 146 using liquid chromatography-mass spectrometry and confirmed that it shared homologous sequences with bacteriocin family proteins. In addition, RNA-sequencing analysis revealed genes encoding cell surface or membrane proteins (SAB0993c, SAB0150, SAB0994c, and SAB2375c) that are involved in the bactericidal activity of lichenicin 146 against Staph. aureus strain RF122 infection as well as those encoding transcriptional regulators (SAB0844c and SAB0133). Thus, our direct in vivo screening platform facilitates simple, convenient, cost-effective, and reliable screening of potential antimicrobial compounds with applications in the dairy field., (Copyright © 2016 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)
- Published
- 2016
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