1. Response of α-glucosidase in gypsy moth larvae to acute and chronic dietary cadmium.
- Author
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Vlahović MS, Mataruga VD, Lazarević JM, Mrdaković MM, Matić DR, Todorović DD, and Ilijin LA
- Subjects
- Animals, Dose-Response Relationship, Drug, Enzyme Activation drug effects, Larva drug effects, Moths enzymology, Cadmium toxicity, Environmental Pollutants toxicity, Insect Proteins metabolism, Moths drug effects, alpha-Glucosidases metabolism
- Abstract
We investigated the effects of acute and chronic treatments with cadmium at 10 μg Cd/g dry food and 30 μg Cd/g dry food on α-glucosidase activity of the 4th instar larvae of Lymantria dispar (Lepidoptera: Lymantriidae) as well as subsequent recovery. Enzyme inhibition was recorded during acute exposure to 30 μg Cd/g dry food and during chronic treatment at the lower metal concentration. After three days recovery from 10 μg Cd/g dry food, the α-glucosidase activity returned to the control level. One-way ANOVA showed that cadmium significantly influenced the activity of α-glucosidase during all treatments. The index of phenotypic plasticity was higher during chronic treatment at 10 μg Cd/g dry food than at 30 μg Cd/g as well as during the recovery. We detected four glucosidase isoforms by NATIVE PAGE. The activities and expressions of the isoforms depended on both larval genotype and cadmium treatment.
- Published
- 2015
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