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The aims of this study were to determine Cd and Pb contents in wild edible mushroom Suillus luteus from the Batak mountain, Bulgaria and thus to assess the Health risk index arisen from the long-term consumption of them. The toxic elements levels were determined by PerkinElmer AAnalyst 800 model atomic absorption spectrometer (PerkinElmer Instruments, Shelton, CT, USA) equipped with Transversely heated graphite atomisation (THGA), and a longitudinal Zeeman effect background corrector was also used. Lumina lamp, Electrodeless discharge lamp (EDL) and hollow cathode lamps were employed as radiation sources. The samples were digested in a microwave closed system Multiwave 3000. The average content of studied elements Pb and Cd in Suillus luteus samples were: 0.066 and 0.052 mg kg-1, respectively. According to the EC Regulation No 629/2018, none of the mushroom samples assayed in this study represented a toxicological risk from lead and cadmium exposure. According to the above results, all calculated Health risk index (HRI) values of heavy metals were within the safe limits for children and adults (HRI < 1). Furthermore, the Total health risk index (THRI) values, were also in the safe limit (THRI < 1). The values HRICd (74%) and HRIPb (26%) have percentage content from THRI. From the point of view of risk assessment, all mushrooms are below provisional tolerable monthly intake (PTMI) for Cd and Pb established by the JOINT FAO/WHO Expert Committee on Food Additives, when temporarily high consumption of mushrooms (100 g/day monthly) is taken under consideration. [ABSTRACT FROM AUTHOR]

The study was conducted on Cambisols soils and wild edible mushrooms (Suillus luteus) from the Batak Mountain, Bulgaria. The total cadmium content in the soils was measured after their decomposition with HCl and HNO3 acids. Mushroom samples were digested with HNO3 and H2O2 in microwave digestion system Mars Xpress. The cadmium levels were determined by Varian 240Z atomic absorption spectrometer with Zeeman background corrector by HGA graphite furnace using argon as inert gas. A correlation/regression analysis was carried out to reveal possible associations between pH, humus content, and total cadmium content of the soils and the concentration of this element in the mushroom samples. It was calculated that BF = 0.37. BF < 1 indicates that the fungus is a metal ion bioreactor. [ABSTRACT FROM AUTHOR]