1. Functional analysis of the C-terminal region of the vacuolar cadmium-transporting rice OsHMA3.
- Author
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Kumagai S, Suzuki T, Tezuka K, Satoh-Nagasawa N, Takahashi H, Sakurai K, Watanabe A, Fujimura T, and Akagi H
- Subjects
- Adenosine Triphosphatases chemistry, Amino Acid Substitution, Arabidopsis, Biological Transport, Cation Transport Proteins chemistry, Mutagenesis, Site-Directed, Onions, Plant Epidermis enzymology, Plant Proteins chemistry, Protein Structure, Tertiary, Protein Transport, Vacuoles enzymology, Adenosine Triphosphatases physiology, Cadmium metabolism, Cation Transport Proteins physiology, Oryza enzymology, Plant Proteins physiology
- Abstract
Rice OsHMA3 is a vacuolar cadmium (Cd) transporter belonging to the P1B-ATPase family and has a long (273aa) C-terminal region. We analyzed the function of the region related to Cd using the transgenic Arabidopsis Col-0 ecotype, which is sensitive to Cd. The OsHMA3 variant containing a truncated (58aa) C-terminal region did not confer Cd tolerance, whereas an OsHMA3 variant containing a longer truncated (105aa) C-terminal region conferred Cd tolerance to transgenic Arabidopsis. We conclude that the C-terminal region, particularly the region containing the first 105aa, has an important role in OsHMA3 activity., (Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.)
- Published
- 2014
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