1. Both oxidative and nitrosative stress are associated with muscle wasting in tumour-bearing rats.
- Author
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Barreiro E, de la Puente B, Busquets S, López-Soriano FJ, Gea J, and Argilés JM
- Subjects
- Animals, Antioxidants analysis, Antioxidants metabolism, Biomarkers analysis, Biomarkers metabolism, Cachexia metabolism, Catalase analysis, Catalase metabolism, Heme Oxygenase (Decyclizing) analysis, Heme Oxygenase (Decyclizing) metabolism, Heme Oxygenase-1, Liver Neoplasms, Experimental metabolism, Male, Muscle, Skeletal enzymology, Muscular Atrophy enzymology, Muscular Atrophy metabolism, Oxidative Stress, Rats, Superoxide Dismutase analysis, Superoxide Dismutase metabolism, Tyrosine analysis, Tyrosine metabolism, Cachexia etiology, Liver Neoplasms, Experimental complications, Muscle, Skeletal metabolism, Muscular Atrophy etiology, Reactive Nitrogen Species metabolism, Reactive Oxygen Species metabolism
- Abstract
Reactive oxygen and nitrogen species (ROS and RNS) have been proposed as mechanisms of cancer-induced cachexia. In this study, we assessed using Western blot analysis the levels of total protein carbonylation (2,4-dinitrophenylhydrazine assay), both malondialdehyde- (MDA-) and 2-hydroxy-4-nonenal- (HNE-) protein adducts, Mn-superoxide dismutase (Mn-SOD), catalase, heme oxygenase-1 (HO-1) and 3-nitrotyrosine formation in gastrocnemius muscles of rats bearing the Yoshida AH-130 hepatoma. In the muscles of the tumour-bearing animals, protein carbonylation as measured by total levels of carbonyl group formation and both HNE and MDA-protein adducts, and protein tyrosine nitration were significantly greater than in control muscles. Protein levels of the antioxidant enzymes Mn-SOD, catalase, and HO-1 were not significantly modified in the rat cachectic muscles compared to controls. The inefficiency of the antioxidant enzymes in neutralizing excessive ROS production may account for elevated markers of protein oxidation and be responsible for the development of both oxidative and nitrosative stress in cancer-induced cachexia.
- Published
- 2005
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