5 results on '"Gonçalves, Lio"'
Search Results
2. SAP30 Gene Is a Probable Regulator of Muscle Hypertrophy in Chickens
- Author
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Bruna Petry, Gabriel Costa Monteiro Moreira, Aline Gonçalves Lio Copola, Marcela Maria de Souza, Fernanda Cristina da Veiga, Erika Cristina Jorge, Jane de Oliveira Peixoto, Mônica Corrêa Ledur, James E. Koltes, and Luiz Lehmann Coutinho
- Subjects
hypertrophy ,siRNA ,knockdown ,skeletal muscle ,C2C12 ,muscle cell growth ,Genetics ,QH426-470 - Abstract
Animals with muscle hypertrophy phenotype are targeted by the broiler industry to increase the meat production and the quality of the final product. Studies characterizing the molecular machinery involved with these processes, such as quantitative trait loci studies, have been carried out identifying several candidate genes related to this trait; however, validation studies of these candidate genes in cell culture is scarce. The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was downregulated in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Modulation of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. RNA-seq analysis identified several upregulated genes annotated in muscle development in treated cells (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation. Here, we provide experimental evidence of the involvement of SAP30 gene as a regulator of muscle cell hypertrophy.
- Published
- 2021
- Full Text
- View/download PDF
3. RGMa can induce skeletal muscle cell hyperplasia via association with neogenin signalling pathway
- Author
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Gerluza Aparecida Borges Silva, Julia Meireles Nogueira, Alinne C. Costa, Erika Cristina Jorge, Aline Gonçalves Lio Copola, and Clara Carvalho E Souza
- Subjects
0301 basic medicine ,Cellular differentiation ,Muscle Fibers, Skeletal ,Nerve Tissue Proteins ,Biology ,GPI-Linked Proteins ,Muscle Development ,Cell Line ,Mice ,03 medical and health sciences ,Myoblast fusion ,0302 clinical medicine ,Downregulation and upregulation ,medicine ,Animals ,Humans ,Myocyte ,Hyperplasia ,Myogenesis ,Gene Expression Regulation, Developmental ,Membrane Proteins ,Skeletal muscle ,Cell Differentiation ,Cell Biology ,General Medicine ,Repulsive guidance molecule A ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,C2C12 ,Signal Transduction ,Developmental Biology - Abstract
Although originally discovered inducing important biological functions in the nervous system, repulsive guidance molecule a (RGMa) has now been identified as a player in many other processes and diseases, including in myogenesis. RGMa is known to be expressed in skeletal muscle cells, from somites to the adult. Functional in vitro studies have revealed that RGMa overexpression could promote skeletal muscle cell hypertrophy and hyperplasia, as higher efficiency in cell fusion was observed. Here, we extend the potential role of RGMa during C2C12 cell differentiation in vitro. Our results showed that RGMa administrated as a recombinant protein during late stages of C2C12 myogenic differentiation could induce myoblast cell fusion and the downregulation of different myogenic markers, while its administration at early stages induced the expression of myogenic markers with no detectable morphological effects. We also found that RGMa effects on skeletal muscle hyperplasia are performed via neogenin receptor, possibly as part of a complex with other proteins. Additionally, we observed that RGMa-neogenin is not playing a role as an inhibitor of the BMP signalling in skeletal muscle cells. This work contributes to placing RGMa as a component of the mechanisms that determine skeletal cell fusion via neogenin receptor.
- Published
- 2021
4. SAP30 Gene Is a Probable Regulator of Muscle Hypertrophy in Chickens
- Author
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Marcela Maria de Souza, Mônica Corrêa Ledur, Luiz Lehmann Coutinho, James E. Koltes, Fernanda Cristina da Veiga, Gabriel Costa Monteiro Moreira, B. Petry, Jane de Oliveira Peixoto, Aline Gonçalves Lio Copola, and Erika Cristina Jorge
- Subjects
Candidate gene ,Gene knockdown ,Muscle Cell Hypertrophy ,knockdown ,Skeletal muscle ,QH426-470 ,Biology ,Cell biology ,Muscle hypertrophy ,medicine.anatomical_structure ,siRNA ,Gene expression ,Genetics ,medicine ,Molecular Medicine ,Myocyte ,C2C12 ,skeletal muscle ,hypertrophy ,Gene ,Genetics (clinical) ,Original Research ,muscle cell growth - Abstract
Animals with muscle hypertrophy phenotype are targeted by the broiler industry to increase the meat production and the quality of the final product. Studies characterizing the molecular machinery involved with these processes, such as quantitative trait loci studies, have been carried out identifying several candidate genes related to this trait; however, validation studies of these candidate genes in cell culture is scarce. The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was downregulated in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Modulation of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. RNA-seq analysis identified several upregulated genes annotated in muscle development in treated cells (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation. Here, we provide experimental evidence of the involvement of SAP30 gene as a regulator of muscle cell hypertrophy.
- Published
- 2021
5. SAP30 Gene Is a Probable Regulator of Muscle Hypertrophy in Chickens.
- Author
-
Petry, Bruna, Moreira, Gabriel Costa Monteiro, Copola, Aline Gonçalves Lio, Souza, Marcela Maria de, da Veiga, Fernanda Cristina, Jorge, Erika Cristina, de Oliveira Peixoto, Jane, Ledur, Mônica Corrêa, Koltes, James E., and Coutinho, Luiz Lehmann
- Subjects
LOCUS (Genetics) ,MUSCULAR hypertrophy ,REGULATOR genes ,MUSCLE growth ,PHENOTYPES - Abstract
Animals with muscle hypertrophy phenotype are targeted by the broiler industry to increase the meat production and the quality of the final product. Studies characterizing the molecular machinery involved with these processes, such as quantitative trait loci studies, have been carried out identifying several candidate genes related to this trait; however, validation studies of these candidate genes in cell culture is scarce. The aim of this study was to evaluate SAP30 as a candidate gene for muscle development and to validate its function in cell culture in vitro. The SAP30 gene was downregulated in C2C12 muscle cell culture using siRNA technology to evaluate its impact on morphometric traits and gene expression by RNA-seq analysis. Modulation of SAP30 expression increased C2C12 myotube area, indicating a role in muscle hypertrophy. RNA-seq analysis identified several upregulated genes annotated in muscle development in treated cells (SAP30-knockdown), corroborating the role of SAP30 gene in muscle development regulation. Here, we provide experimental evidence of the involvement of SAP30 gene as a regulator of muscle cell hypertrophy. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
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