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Start Over Author "Belew, Micah Y." Topic c. elegans
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1. Imaging Lipid Metabolism in Live Caenorhabditis elegans Using Fingerprint Vibrations.

Author

Wang, Ping, Liu, Bin, Zhang, Delong, Belew, Micah Y., Tissenbaum, Heidi A., and Cheng, Ji‐Xin

Subjects
LIPID metabolism, CAENORHABDITIS elegans, RAMAN scattering, MULTIVARIATE analysis, OXIDATION, CHOLESTEROL
Abstract

Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long-standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome-related organelles in intestinal cells are sites for storage of cholesterol in C. elegans. [ABSTRACT FROM AUTHOR]

Published
2014
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