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The Squash Leaf Curl China Virus (SLCCNV) and Tomato Leaf Curl New Delhi Virus (ToLCNDV) are species of Begomovirus (whitefly-vectored Geminiviridae) and cause serious damage to the cucurbit crops of the genus Cucurbita in the areas of South and Southeast Asia, across Asia, the Middle East and the Mediterranean, respectively. Cucurbita moschata's inbred line AVPU1426, developed at the World Vegetable Center (WorldVeg) from a Bangladeshi landrace through pedigree selection, was observed to be resistant to both begomoviruses in field tests conducted at the WorldVeg Research and Training Station, Kasetsart University, Kamphaeng Saen, Thailand, which is a hotspot for these viruses. When AVPU1426 was tested for reaction to inoculation by viruliferous whiteflies with Squash Leaf Curl Philippines Virus (Taiwan strain) (SLCPV-TW) in the screen net-house at WorldVeg headquarters in Taiwan, it showed good resistance, though SLCPV-TW DNA-A could be detected in all inoculated plants, indicating that it did not show immunity to this virus. The objective of this study was to validate the resistance to SLCCNV and ToLCNDV in AVPU1426 by using whitefly-mediated inoculations to determine the mode of inheritance of the resistance. The results showed that AVPU1426 was resistant to SLCCNV and ToLCNDV. Upon crossing AVPU1426 with a susceptible check variety, Waltham Butternut, the resistance to both begomoviruses was observed to be conferred by a single recessive gene. This open-pollinated pumpkin line AVPU1426 bears flat round, mottled green immature fruits with yellow flesh in mature fruit. The fruit yield of AVPU1426 (20.74 t/ha−1) was comparable to 'Rajah' (18.61 t/ha−1), a recently released commercial F1 hybrid of East-West Seed (EWS). The fruit of AVPU1426 were estimated to have a good average β-carotene content (1.57 mg/100 g fresh weight). This line is a good source to breed pumpkins resistant to the three begomoviruses. [ABSTRACT FROM AUTHOR]