1. MGB Block ARMS Real-Time PCR for Diagnosis of CYP2C19 Mutation in a Chinese Population
- Author
-
Zhu Xiao-ya, Huang Tao-Sheng, Jiang Xi-Wen, and Liu Yue
- Subjects
Genetics ,Chinese population ,business.industry ,030204 cardiovascular system & hematology ,Biochemistry ,03 medical and health sciences ,Computational Mathematics ,0302 clinical medicine ,Real-time polymerase chain reaction ,030220 oncology & carcinogenesis ,Block (telecommunications) ,Mutation (genetic algorithm) ,Medicine ,business ,Molecular Biology - Abstract
Background:CYP2C19 is an important genetic factor modulating clopidogrel dose requirement.Objective:Therefore, a simple and economic genotyping method for predicting the clopidogrel dose of patients would be useful in clinical applications.Methods:In this study, the MGB blocker ARMS real-time PCR contained two forward primers, two MGB blockers and a common reverse primer have been used for CYP2C19*2, *3 and *17 substitutions.Results:Results showed that heterozygotes and homozygotes of CYP2C19*2, *3 and *17 could be distinguished by the MGB blocker ARMS real-time PCR successfully. In the Chinese population, patients with allele frequencies of CYP2C19*2, *3, and *17 are 18.43%, 3.03% and 0.76%, respectively.Conclusion:This study indicates that the MGB blocker ARMS real-time PCR will be a simple, economical method for the rapid detection of SNPs in CYP2C19.
- Published
- 2019