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Objective Defects in the human solute carrier family 26 member 4 (SLC26A4) gene are reported to be one of the causes of congenital hypothyroidism (CH). We aimed to identify SLC26A4 mutations in Chinese patients with CH and analyze the function of the mutations. Methods 273 patients with primary CH were screened for 21 CH candidate genes mutations by targeted next-generation sequencing. All the exons and exon-intron boundaries of SLC26A4 were found and analyzed. The function of 6 missense mutation in SLC26A4 were further investigated in vitro. Results Among 273 patients with CH, 7 distinct SLC26A4 heterozygous mutations (p.S49R, p.I363L, p.R409H, p.T485M, p.D661E, p.H723R, c.919-2A>G) were identified in 10 patients (3.66%, 10/273). In vitro experiments showed that mutation p.I363L, p.R409H,p.H723R affect the membrane location and ion transport of SLC26A4, ,while p.S49R did not.Mutation p.T485M and p.D661E only affect the ion transport, but has no effect on the membrane location. Conclusion Our study indicated that the prevalence of SLC26A4 mutations was 3.66% in the Chinese patients with CH. Five mutations (p.I363L, p.R409H, p.T485M, p.D661E and p.H723R) impaired the membrane location or ion transport function of SLC26A4，which revealed important role of the Ile363,Arg409, Thr485, Asp661, His723 residues in function of SLC26A4. Because these mutations are heterozygous mutations, the pathogenesis of these patients cannot be explained, and the pathogenesis of these patients needs further study.

This paper presents an Electric bicycle controller,which is designed on the basis of Programmable System-On-Chip used in BLDCM. The system structure is described. The software and drive circuit are designed. Several modules integrated in PSOC,such as PWM,AD and timer modules,are used to Electric bicycle controller the brushless DC motor can be controlled by PWM output with the help of HALL-position sensors.

Designed a Control Technology in Coal Handling System of Xinji Cogeneration Power Plant, the advanced nuclear scale is to measure the instantaneous flow of two coal conveyor belt, The coal plow and the belt switch signal is collected. Depending on the signal , the coal is accumulated to the different coal bunker. Two belts of coal was send to five different the coal bunker and was accumulated, this is conducive to the analysis of the economic indicators, so to achieve the purpose of power plant energy consumption.

Design a system of PCB engraving machine using MCU. Design in detail mechanical structure and the system scheme of the engraving machine. Write a software which can converse the file of PCB drawing software output to program for SCM and exchange data between PC and SCM. Choose the right step motor and design driven circuit of step motor.

We designed a set of non-destructive system which is based on ray inspection systems, it mainly used to detect the Green Channel Cargo, and the result will be given out within 30 seconds. The ray coefficient of the Green Channel Cargo are different from the other transport cargo, so we can measure the absorption coefficient and compare it with the standard samples which is pre-built. The moved radioactive source and detector are designed, in order to prevent the mixed goods, we designed the removable radioactive sources and detectors, so the goods can be scanned up and down and around. Once the goods are mixed together, the ray coefficient which is calculated synchronously will be abnormal, parking and manual inspection is a supplementary means, so as to achieve a low-cost non-destructive testing.

The mixed solutions of brilliant blue and indigotine are prepared and the fluorescence spectra of them are experimentally measured. The serious overlapping spectra of brilliant blue and indigotine are solved by means of the first-derivative fluorescence spectrometry. The wavelet coefficients, obtained by compressing the spectral data using wavelet transformation (WT), are taken as inputs to establish the radial basis function neural network (RBFNN). The neural network model can realize simultaneous determination of brilliant blue and indigotine, and the mean relative errors of both compounds are 1.84% and 1.26%, respectively.