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Otitis media is a destructive & persistent disease with irreversible sequelae. It is associated with bacterial or fungal aetiology. Objectives: 1) To identify bacterial isolates causing ear infection and their antibiogram. 2) To identify fungal isolates 3) To screen all smears for Acid Fast Bacilli (AFB). Materials and Methods: This study was conducted for 1 year. Pus was collected with sterile swab before commencement of antibiotics, from 98 patients of otitis media, attending ENT OPD. Gram’s staining & Gabbet’s staining (Modified ZN staining) was done for all the samples. Bacterial & fungal isolates were identified by standard methods. Antibiotic susceptibility testing of bacterial isolates was carried out by Kirby Bauer’s disc diffusion method. Results: Age of the patients studied, ranged from 2 to 68 years. Of the 98 patients, 48 were females and 50 were males. Bacterial isolates and their incidence were: Staphylococcus aureus(31%), CONS(6%), Pseudomonas species(20%), Klebsiella species(7%), Proteus species(5%). 4 fungi were isolated. 2 samples showed acid fast bacilli on Gabbet’s staining. Conclusions: The most common organisim causing Otitis media in this study was Staphylococcus aureus (MSSA). Antibiotic treatment should be individualized depending on antibiotic sensitivity report, to reduce chronicity, complications & morbidity. It also showed in the study that, doing acid fast staining routinely could be of help as it helped to diagnose two cases of pulmonary tuberculosis. Keywords: ENT- ear, nose, throat, OPD- out patient department, CONS- coagulase negative staphylococci, MSSA- methicillin sensitive staphylococcus aureus, AFB- acid fast bacilli

Introduction: Resistance to multiple antibiotics among Gram negative bacilli (GNB) is high in India. Tigecycline, a glycylcycline antibiotic is a newer treatment option for emerging single or multidrug-resistant (MDR) GNB. Material and Methods: All the samples received in the Microbiology laboratory were processed according to standard protocols. Once identified, they were subjected to antibiotic susceptibility testing by disk diffusion method on Mueller Hinton agar. We evaluated the in vitro activity of tigecycline and compared it against other antimicrobials. Among 356 isolates, [Escherichia coli (126), Klebsiella spp (61), Pseudomonas aeruginosa (133), Acinetobacter spp. (23) and Proteus (13)] from patients with, skin and soft tissue (SSTI) including surgical site, urinary tract and respiratory infections were isolated in our laboratory, Department of Microbiology, Mandya Institute of Medical Sciences, Mandya. Susceptibility to antimicrobials besides tigecycline included amikacin, imipenem, levofloxacin, meropenem, and piperacillin/tazobactam was determined by disk diffusion method. Results: Tigecycline was active against all E. coli and Klebsiella spp by disc diffusion method. MDR Acinetobacter spp. showed lower susceptibility (70.6%) to tigecycline and Pseudomonas spp and Proteus spp showed high resistance. Increased resistance was seen to other antimicrobials among Extended Spectrum Beta Lactamases(ESBL) producing E. coli, Klebsiella spp., Metallo Beta Lactamase (MBL) producing P. aeruginosa. Discussion: Tigecycline shows high potency against Gram-negative bacilli belonging to family Enterobacteriaceae in whom multi-drug resistant strains have emerged as important nosocomial pathogens. Tigecycline is highly active against Enterobacteriaceae, regardless of the presence or absence of ESBLs, >99% of all of the tested strains were inhibited.In the present study we found that tigecycline had good activity against tested E.coli and Klebsiella spp. Conclusion: Ti