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Simple Summary Castration is used to improve the management of buffalo calves and their meat quality. Despite the growing interest in livestock welfare, water buffaloes have not been studied as frequently as other species. General anaesthesia produces complete unconsciousness, insensitivity to pain and muscle relaxation. The quality and safety of general anaesthesia may be improved using sedatives prior to the induction of general anaesthesia. The aim of this study was to evaluate the effect of two different intramuscular doses of xylazine for premedication, followed by ketamine induction, in water buffalo calves undergoing isoflurane maintenance anaesthesia for routine castration surgery. Based on the results obtained, we can conclude that both protocols are safe for buffalo castration, but the higher dose protocol is recommended for buffalo orchiectomy. Abstract Castration is one of the most frequently performed surgical procedures in livestock. All methods of castration are associated with a significant degree of pain, affecting the patients’ welfare. Limited information on species-specific protocols in buffaloes is available. Considering the importance of this species and the scarcity of reports available, the objective of this study was to evaluate the effects of two different intramuscular doses of xylazine for premedication in water buffalo calves undergoing isoflurane maintenance anaesthesia for routine castration surgery. Twenty clinically healthy male water buffaloes undergoing bilateral orchiectomy were randomly assigned to two groups of 10 animals each: Group 1 was premedicated with xylazine 0.1 mg/kg bw i.m. and Group 2 was premedicated with xylazine 0.15 mg/kg bw i.m. Anaesthesia was induced by ketamine (2 mg/kg bw i.v.) and maintained with isoflurane-oxygen-air in both groups. The HR, RR, presence/absence of ataxia, sedation, depth of anaesthesia, muscle relaxation status, response to acoustic and tactile stimuli, eyelid reflex, extent of salivation and stimulus were evaluated every 5 min. Both protocols provided consistent sedation and were safe. Faster and more satisfactory sedation was recorded when xylazine was administered at 0.15 mg/kg bw i.m., leading us to recommend this dose for buffalo orchiectomy.

Background: Capsulitis leads to the release of inflammatory mediators into the joint causing capsular fibrosis and osteoarthrosis (OA). Strain elastosonography (SE) measures the elasticity of tissue evaluating its strain to an operator-dependent deformation. Aims of the study were to assess the feasibility of SE for evaluating the distal attachment of the joint capsule (DJC) of fore fetlocks in sound horses and in horses with fetlock OA and to evaluate differences in their elastosonographic aspect. After a whole lameness examination, fore fetlocks DJC were assigned to Group S and Group P and examined by two operators using SE. The inter-rater reliability (IRR), the intraclass (intra-CC) and interclass (inter-CC) correlation coefficients were used to compare colour grading scores, repeatability, reproducibility of elasticity index (EI) and strain ratio (SR). The same parameters were compared between groups. Findings were significant for P < 0.05. Results: Forty-one horses were included, 11 in Group S, 30 in Group P (16 with bilateral OA, 8 left and 6 right OA). IRRs ranged from good to excellent. For transverse and longitudinal scans, the EIs of Group S were 0.57-0.51, SRs were 0.32-0.28. Inter-CC and intra-CC were always > 0.8. In Group P, EI was 1.09-0.89, SRs was 0.78-0.72 with intra-CC and inter-CC >0.57. Significative differences of EI and SR were detected between groups, and between Group S and the affected limb of Group P.Conclusions: SE is a useful technique for evaluating the DJC, with good repeatability and reproducibility. DJC appears softer in sound horses.

Background Capsulitis leads to the release of inflammatory mediators in the joint, causing capsular fibrosis and osteoarthritis (OA). Strain elastosonography (SE) measures the elasticity of tissue by evaluating its strain in operator-dependent deformation. The aims of the study were to assess the feasibility, repeatability, and reproducibility of SE for imaging the distal attachment of the joint capsule (DJC) of metacarpophalangeal joints in sound horses (Group S) and in horses with metacarpophalangeal OA (Group P) and to evaluate differences in the elastosonographic patterns of these horses. After a whole lameness examination, fore fetlock DJCs were assigned to Group S and Group P and were thereafter examined by two operators using SE. Qualitative (i.e., colour grading score) and semi-quantitative (i.e., elasticity index (EI) and strain ratio (SR)) methods were used to evaluate the elastograms. The inter-rater reliability (IRR), intraclass correlation coefficient (intra-CC) and interclass correlation coefficient (inter-CC) were used to compare colour grading scores and the repeatability and reproducibility of EI and SR outcomes. The same parameters were compared between groups. P Results Forty-one horses were included: 11 were in Group S and 30 were in Group P (16 with bilateral OA, 8 with left OA and 6 with right OA). IRR outcomes ranged from good to excellent. For transverse and longitudinal ultrasound scans, the colour grading score of Group S was significantly higher than the metacarpophalangeal DJCs of Group P. Both Inter-CC and intra-CC were higher in Group S than in Group P, with values always > 0.8. Significative differences in EI and SR were detected between groups and between Group S and the affected limb of Group P; values were lower in Group S than in Group P. Conclusions SE can be a useful technique for evaluating DJCs, with good repeatability and reproducibility. DJCs appear softer in sound horses.

Pain management is necessary for all surgical procedures. Little scientific evidence about drug efficacy in donkeys is available. The aim of this study was to evaluate the analgesic effect of butorphanol in donkeys undergoing orchiectomy under total intravenous anaesthesia with guaifenesin-ketamine-detomidine. A randomized blinded prospective clinical trial (Protocol n. 2021/0000338), was carried out on 18 clinically healthy donkeys undergoing bilateral orchiectomy. Patients were assigned to Group D (n = 8) or Group DB (n = 10) if receiving intravenous detomidine or detomidine-butorphanol respectively, before induction of general anaesthesia with ketamine-diazepam. Intraoperative muscle relaxation, nystagmus, palpebral reflex, heart and respiratory rate, and non-invasive blood pressure were evaluated every 2 min, time to prepare the patient, duration of surgery and anaesthesia and recovery score were recorded. Group D had significantly longer surgical time, higher heart rate, higher systolic and mean blood pressure (p &lt, 0.05, repeated measure ANOVA), increased muscle rigidity and expression of palpebral reflex (p &lt, Mann–Whitney U test) than group DB. Top-ups with thiopental were statistically higher in Group D. Butorphanol and detomidine together produced a more stable anaesthetic plan. The low dosage of opioid and alpha-2-agonists and reduced rescue anaesthesia are responsible for a safer and more superficial anaesthesia, which is mandatory under field conditions.

Simple Summary The equine prepuce is characteristic for having two infoldings that allow for substantial accommodation of the penis during detumescence and permit erection. Segmental posthetomy of the equine prepuce is a well described technique indicated for penile sheath injuries or lesions (neoplasms, granulomas, or scar tissue) that do not involve the underlying penile tunics. This surgical technique can be performed with variable extent of excision (i.e., the Adam’s procedure or subtotal posthetomy). The authors describe their experiences and results with different levels of preputial resection for treating four Equidae stallion patients with different preputial/pathologies. Abstract Segmental posthetomy, also referred to as circumcision, reefing or posthioplasty, consists of removing a circumferential segment of the internal preputial lamina (internal preputial fold) followed by end-to-end anastomosis of skin edges. The purpose of this case series is to describe the successful outcome of segmental posthetomy for treating different diseases involving the internal or/and external preputial fold, while restoring the normal telescopic function. In this paper, we report the first case of complete degloving injury of the equine penis in the literature (case 1) and describe three different common lesions of the equine prepuce/penis (preputial scar tissue in case 2, preputial sarcoid in case 3 and penile/preputial wound in case 4). The amount of prepuce (safe minimums) that can be removed from a stallion without disrupting the proper telescopic function of the internal/external preputial fold and normal copulatory ability, has not been established. In this case series, all Equidae stallions maintained the telescopic function after preputial surgical resection. However, the surgeon must carefully evaluate every single case, especially when performing the Adam’s procedure.

The aim of the study was to investigate the breed predisposition and the diagnostic and surgical management of horses referred for cryptorchidism. The breed, localization of retained testis, diagnosis, type of surgical treatment and complications were analyzed. Seventy horses were included in the study, the Western Riding horse breeds were the most affected (Quarter Horse 34/70, 48.5%, Appaloosa 9/70, 12.8%). In unilateral cryptorchids (65/70, 92.8%) the most common location for a retained testis was the left abdomen (28/65, 43%), while in bilateral cryptorchids (5/70, 7.1%), bilateral abdominal retention was the most frequent (3/5, 6%). Information about testis localization was achieved through transabdominal ultrasound (30/49 cases, 61.2%), through per rectum palpation (21/49 cases, 42.9%) and through inguinal palpation (14/49 cases, 28.9%). Cryptorchidectomy was achieved with standing laparoscopy (44/70 cases, 62.8%), or with open inguinal orchiectomy in general anesthesia (26/70 cases, 37.2%). Complications during laparoscopy were spleen puncture (1/44, 2.2%), a self-limiting bleeding from the spermatic cord (10/44 cases, 22.7%), hyperthermia (3/44 cases, 6.8%), and emphysema (15/44, 34%). During inguinal open cryptorchidectomy difficulties with identifying the inguinal testis during surgery (8/26 cases, 30.8%) and a moderate and self-limiting swelling of the inguinal region after surgery (17/26, 65.4%) were observed. For orchiectomy, a standing laparoscopy was confirmed as the preferred procedure for an abdominally retained testis with almost no complications.

Mules are hybrids bred from the mating of a jack donkey and a horse mare, known for their strength and resistance and still used to work in agriculture. Although they have been for long considered sterile, evidence of estrus cycle has been demonstrated together with abnormal behavior related to ovarian activity. In this study, a bilateral standing laparoscopic ovariectomy technique using the LigaSure technology was applied in 10 mare mules for treating unwanted behavioral patterns. The technique was effectively performed on these animals avoiding the risk of general anesthesia, and the use of the LigaSure technology allowed good hemostasis and reduced surgical time. Owners declared to be satisfied with the resolution of the behavior.

Objective To analyse the effectiveness of intrafunicular lidocaine and intravenous flunixin for reducing pain and signs of stress in lambs undergoing surgical castration. Design Randomised controlled trial. Setting One university teaching hospital in Italy. Participants 30 healthy male lambs, 9–12 weeks old. Intervention Allocation to five groups: a control group (C), undergoing general anaesthesia but not castration; a surgery group (S), undergoing orchiectomy without analgesic treatment; a surgery-lidocaine group (SL), undergoing orchiectomy and receiving intrafunicular 2 per cent lidocaine solution; a surgery-flunixin group (SF), undergoing orchiectomy and receiving intravenous flunixin; a surgery-flunixin-lidocaine group (SFL), undergoing orchiectomy and receiving both intrafunicular lidocaine and intravenous flunixin. Main outcome measures Nociception and stress were assessed through intraoperative indicators, serum cortisol concentration, glycaemia, behaviour, immune response and clinical evaluation of the heart rate (HR), respiratory rate and rectal temperature after surgery. Results Groups S and SL showed increased values of intraoperative HR, mean arterial pressure and postoperative cortisol concentration. In group SFL, cortisol values were similar to those of group C. No other difference could be detected. Conclusions The combination of intravenous flunixin and intrafunicular lidocaine reduced the pain and discomfort of lambs castrated under general anaesthesia. Intrafunicular lidocaine alone did not prevent pain or discomfort associated with castration. Trial registration number 30/2012/CEISA/COM.

This study aimed to evaluate the analgesic effect of intrafunicular lidocaine during orchiectomy in isoflurane-anesthetized donkeys. For this purpose, 10 adult healthy donkeys were chosen from Martina Franca donkey population. Each donkey underwent two surgical procedures of monolateral orchiectomy under general anesthesia. Starting isoflurane vaporizer setting was 1.5% to maintain a light plane of anesthesia. Regional anesthesia was performed by injecting 10 mL of 2% lidocaine plus adrenalin or an equivalent volume of saline solution into the spermatic cord. According to what was injected into the funiculus before the surgical procedure, each donkey was once assigned to the group L (lidocaine) and once to the group S (saline). End-tidal isoflurane and standard physiological parameters were measured. Compared with groups, monolateral orchiectomy increased mean heart rate in group S; during surgical procedure, the end-tidal isoflurane was significantly lower in group L. No differences were found regarding mean arterial blood pressure, respiratory rate, recovery quality, and metabolic parameters between groups during procedures. In isoflurane-anesthetized donkeys, intrafunicular injection of lidocaine before castration appears to decrease intraoperative nociception and significantly reduces the concentration of the volatile agent to obtain a sufficient surgical anesthesia.

This case report describes surgical treatment of 3 cases of septic chronic tenosynovitis of the tarsal sheath with fragmentation of the sustentaculum tali in 3 horses. Diagnosis was based on clinical signs, radiographic and ultrasonographic findings, synovial fluid cytology, and bacteriology. In each case, treatment consisted of tenovaginoscopy of the tarsal sheath performed with the horse under general anesthesia and regional intravenous perfusion of antibiotics. Follow-up data obtained between 12 and 46 months after surgery reported a good outcome, with a good cosmetic appearance in all cases described. Two horses returned to their intended use at 44 and 46 months, and 1 horse reportedly showed a remarkable functional improvement after 12 months. Despite the poor prognosis associated with the chronicity of the lesions, the presence of adhesions and the degree of involvement of the lateral digital flexor tendon, tenovaginoscopy of the tarsal sheath and regional antimicrobial therapy were successful therapeutic options for treating septic tenosynovitis of the tarsal sheath with fragmentation of the sustentaculum tali.

A 15-year-old gelding was referred for a florid, cauliflower-like ulcerated mass, enclosing penis and prepuce together with penile urethra showing a malodorous purulent and blood-stained discharge and larvae infestation.En blocextensive resection of the penis and prepuce, without penile retroversion or pexy to ventral abdomen associated with a permanent perineal urethrostomy, was performed. Histology of the mass revealed a squamous cell carcinoma of penis and prepuce. The surgical technique that was adopted is a modified version of that already described that allows a more proximal resection of the penile body and is a valid option for treating advanced SCC lesions involving the penis. Early postsurgical complications (mild strangury, haemorrhage from the urethrostomy site and its partial dehiscence, and infection of the abdominal wound) were managed with a medical treatment and resolved within 5 to 12 days. Three years after surgery the horse is in good body condition and does not show any sign of recurrence or disorders related to the surgery.

Objective To report laparoscopic-assisted cystotomy and inguinal cystostomy for treating bladder urolithiasis in a gelding. Study Design Clinical report. Animals Twelve-year-old Appaloosa gelding. Methods A laparoscopic approach was used to locate and grasp the bladder, which was exteriorized through an enlarged instrument portal for cystotomy to remove the calculus. During withdrawal, the calculus fragmented into multiple pieces. To avoid urethral occlusion and facilitate elimination of these fragments, an inguinal cystostomy was created. The seromuscular layer of the bladder was circumferentially secured to the abdominal fascia and bladder mucosa margins were sutured to the skin. Results At 2-year follow-up, the gelding was in good condition with mild urine scald of the left ventral abdomen. Conclusion Laparoscopic-assisted cystotomy was used for calculus removal and inguinal cystostomy for successful fragment elimination.