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Background: Haruan fish (Channa striatus) extract (HFE) contains all the essential amino acids and fatty acids that it is believed to have therapeutic value, accelerate wound healing and anti-inflammation. This study was aimed to examine the viability of odontoblast MDPC-23 cell lines following the addition of HFE in toxin of Lactobacillus sp. and/or Ca(OH)2. Materials and Methods: Firstly, to find antiproliferative effective doses, MDPC-23 cells were treated with HFE. The cell viability was measured by MTT assay on 24 h after the last treatment. While cell death was induced by addition toxin and/or Ca(OH)2 following adding antiproliferative effective doses of HFE (25.0; 50.0; and 100 µg/mL). Untreated cells were used as control. Result: Adding of HFE at range 25.0 till 100 µg/mL increased the MDPC-23 cells viability. MDPC-23 on toxin and/or Ca(OH)2 reported decrease the viability of cells, while supplemented with HFE significantly increase in cell viability compared to untreated cell (p

this study aims to evaluate effectiveness difference between convex triangular and rectangular cross section rotary instruments on root canal Enterococcus faecalis bacteria colonies. Samples mandibular premolar teeth with one narrow and straight root canal divided into 3 groups : instrumented with convex triangular cross section rotary instruments, rectangular cross section rotary instruments, and convex triangular cross section manual instruments. Each group consisted of 9 samples. Standardized teeth length, instrumented to #15 K-file and sterilized. E. faecalis suspension in BHIB was standardized then 27 root canals contaminated. Root canals were incubated for 21 days. Every 48 hours root canals added with BHIB. Initial samples were collected, plated in agar culture medium and incubated for bacterial count. and then bacterial count was performed again. Data were collected and analysed using Paired T-test and ANOVAThe results showed there are significant difference on Enterococcus faecalis bacteria colonies amount before and after preparation with all instruments that was used and bacterial reduction amount in root canals were greater on convex triangular cross section instruments than on rectangular cross section instruments even there was no significant difference.

This study was aimed to determine the effect of epoxy resin-based and MTA-based sealer on fiber post bond strength in the root canal wall.Samples are mandibulary first premolar, with a single root canal. They are divided into three groups: negative control group which is a gutta percha obturation without sealer, gutta percha obturation with epoxy resin based sealer [AH Plus], and gutta percha obturation with MTA-based sealer [MTA Fillapex] groups. Samples were decoronated, prepared, obturated and then stored in the incubator at room temperature for one week, the post space were prepared for fiber post insertion. Samples were mounted in the PVC pipes before insertion. Samples were stored in the incubator for one day before bond strength testing. Universal testing machine was used with the speed of 0,5 mm/minutes. Data were collected and analysed using ANOVA.The result showed that the fiber post bond strength in the root canal obturated with epoxy resin-based sealer was higher [12.311 N/mm2] than MTA-based sealer [10.786 N/mm2], but that result was not statistically significant. Therefore, it was concluded that the root canal obturated with epoxy resin-based sealer did not yield a significant bond strength [p = 0.689] compared to MTA-based sealer [p> 0.05].

Diastema in the anterior teeth is a common aesthetic problem, which interfere confidence of patients. The cause of a diastema let known with certainty in advance before preparing the treatment plan. Management diastema closure on anterior teeth can use veneer. Veneer restorations can be made by direct technique with composite materials and indirect use porselen material. The purpose of this literature review is to discuss case management of diastema in the anterior teeth using veneer restorations with direct and indirect techniques.

This study aims to determine the characteristics of the microhardness impairment root canal dentin after application with different types of EDTA. Samples mandibular premolar teeth with one root canal, each divided into 4 groups: EDTA solution, EDTA gel, EDTA cream and negative control; and each group consisted of 6 samples. The teeth were decoronated at cementoenamel junction (CEJ), prepared by the crown down pressureless technique, cut along longitudinal direction, and each sample was attached to selfcured acrylic and then soaked in distilled water. Samples were taken early microhardness measurement by means of Digital Vickers Microhardness Tester. The sample is then applied to the appropriate group of materials EDTA for 5 minutes, except for the negative control group, soaked in saline solution for 5 menit, then performed the final measurement of microhardness of dentin. The results of measurements taken from the average value of measurements made at 3 points, coronal, middle and apical. Data were collected and analyzed using ANOVA and Tukey’s Post Hoc test.The results showed there are differences in dentin microhardness decrease significantly in all treatment groups compared to the negative control group (p 0.05 which means there is no difference in microhardness reduction in dentin significantly among the test group.

This study aim to compare the smear layer clearance on the apical third of the root canal walls instrumented with convex triangular cross- section designed hand instruments, rectangular cross-section and convex triangular cross-section designed rotary instruments. Mandibular premolars that met the inclusion criteria were divided into 3 groups of 10 samples, and prepared using convex triangular cross section design hand instruments, convex triangular cross-section and rectangular cross-section design rotary instruments. After preparation and drainage, the sample was cut into halves using a stainless steel chisel, a section was randomly selected and examined using SEM with 1000 X. This study showed that the smear layer clearance of hand instrument group was the lowest compared to other two groups, the rotary instruments with rectangular cross-section design showed the highest percentage smear layer clearance among the three groups. It was concluded that root canal preparation using a rotary instrument design convex triangular cross section produces hygiene apical third of the canal walls of the smear layer significantly compared to manual instruments designed convex triangular cross section and root canal preparation with a rotary instrument design of rectangular cross section produces hygiene apical third of the wall root canal of the smear layer significantly compared convex design of triangular cross section. Penelitian ini bertujuan untuk menilai perbedaan kebersihan sepertiga apikal dinding saluran akar dari smear layer antara preparasi yang menggunakan hand use dan rotary instrument design convex triangular cross section dengan rotary instrument design rectangular cross section . Sampel gigi premolar rahang bawah yang memenuhi kriteria inklusi dibagi 3 kelompok, masing-masing sebanyak 10 sampel, lalu dipreparasi dengan menggunakan instrumen hand use design convex triangular cross section , rotary instrument design convex triangular cross section , dan rotary instrument design rectangul a r cross section. Setelah dipreparasi, sampel dikeringkan, gigi dibelah dua menggunakan stainless steel chisel . Salah satu belahan gigi dipilih secara acak, kemudian diamati dengan SEM (pembesaran 1000x). Hasil penelitian menunjukkan bahwa kelompok hand use instrument mempunyai persentase kebersihan smear layer yang paling rendah, sedangkan kelompok rotary instrument rectangular cross section memiliki persentase yang paling tinggi. Disimpulkan bahwa preparasi saluran akar menggunakan rotary instrument design convex triangular cross section menghasilkan kebersihan sepertiga apikal dinding saluran akar dari smear layer secara signifikan dibandingkan manual instrument design convex triangular cross section dan preparasi saluran akar dengan menggunakan rotary instrument desain rectangular cross section menghasilkan kebersihan sepertiga apikal dinding saluran akar dari smear layer secara signifikan dibandingkan desain convex triangular cross section .