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Made available in DSpace on 2020-12-12T02:13:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Difference in blood and peritoneal glucose (DBPG) is used in clinical practice to support a diagnosis of septic peritonitis in horses. It is inexpensive, easy and rapid to perform. The aim of this study was to evaluate the accuracy of the DBPG to differentiate between septic and non-septic peritonitis in horses. Blood and peritoneal fluids were harvested from suspected animals. Plasma and peritoneal glucose levels, total nucleated cell count, direct microscopic and microbiological examinations of the peritoneal fluid were evaluated. Using DBPG levels, the animals were classified into two groups: difference ≥ 50 mg/dL (positive test) and difference < 50 mg/dL (negative test). Positive microbiological examination and/or presence of bacteria in direct microscopic examination was used as a gold standard to detect septic peritonitis. The accuracy parameters analysed were: sensitivity, specificity, and positive/negative predictive values, for which the results were respectively: 0.23, 0.91, 0.60 and 0.67. Due to poor accuracy, other cut-off margins and peritoneal glucose concentrations were evaluated. The test was considered most accurate when the DBPG was zero with sensitivity, specificity, and positive/negative predictive values of 0.85, 0.82, 0.73, 0.90 respectively. Peritoneal glucose concentrations alone were not a reliable feature to detect peritonitis. DBPG ≥50 mg/dL, widely used for the diagnosis of septic peritonitis, does not have a good accuracy and the DBPG = 0 has a better accuracy for detecting the disease. Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) Department of Internal Medicine Botucatu Medical School Univ. Estadual Paulista (Unesp) Department of Veterinary Clinics School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) Department of Internal Medicine Botucatu Medical School Univ. Estadual Paulista (Unesp) Department of Veterinary Clinics School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) FAPESP: 2016/08712-8 e 2018/ 16013-8

Made available in DSpace on 2022-04-29T08:36:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Tuberculosis remains one of the most important infectious diseases with well-known zoonotic nature that affect humans, wildlife, and domestic animals, including goats. Nonetheless, no intradermal tuberculin test has been standardized for caprine diagnosis of tuberculosis. The present study investigated the intradermal comparative cervical tuberculin test (ICCTT) in the diagnosis of tuberculosis among 60 goats from farms with history of tuberculosis. The cutoff applied to goats was based on a study where goats had been experimentally infected with Mycobacterium bovis and Mycobacterium avium. Clinical examination, bacteriological culture, and histopathological staining were assessed to the diagnosis. Isolates compatible with mycobacteria were subjected for molecular diagnosis based on gyrB-restriction fragment length polymorphism (RFLP) analysis and PCR restriction-enzyme analysis (PRA) of hsp65 gene by BstEII and HaeIII, namely PRA-hsp65 assay. From all goats, 60% (n = 36/60), 3.3% (n = 2/60), and 36.7% (n = 22/60) showed positive, inconclusive, and negative reactions, respectively. Out of 36 goats with ICCTT positive, 75% (n = 27/36) had isolation of mycobacteria and were detected M. bovis by gyrB-RFLP. Molecular diagnosis and histopathological findings compatible with tuberculosis showed 86.1% (n = 31/36) concordance with the ICCTT. When compared ICCTT with M. bovis isolation, gyrB-RFLP, and histopathology, the better arithmetic means of sensitivity and specificity were 2.5 mm for ICCTT compared with M. bovis isolation and gyrB-RFLP, and 4.55 mm when compared with histopathology. Both receiver operating characteristic (ROC) curves presented statistical significance (P < 0.001). The identification of other mycobacteria, e.g., M. kansasii, M. flavescens, M. avium, M. florentinum, M. lentiflavum, M. simiae, and Corynebacterium pseudotuberculosis, not influenced positive results in ICCTT. The concordance between bacteriological, histopathological, and molecular identification with ICCTT findings indicate that the tuberculin test may be used as a valuable tool for diagnosis of caprine tuberculosis and reinforce the importance of association of methods to diagnostic of the disease from animal origin. Coordination of Sustainable Rural Development Secretariat of Agriculture and Supply of State of São Paulo, SP Animal Pathology Department School of Veterinary Medicine and Animal Sciences University of São Paulo-USP, SP Department of Preventive Veterinary Medicine and Animal Science USP, SP Biological Institute of São Paulo Secretariat of Agriculture and Supply of State of São Paulo, SP Tuberculosis and Mycobacteriosies Laboratory Adolfo Lutz Institute, SP Departament of Clinics USP, SP Veterinary Medicine Academic Unit Federal University of Campina Grande UFCG, PB Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP, SP Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP, SP

Made available in DSpace on 2022-05-01T07:58:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Bloody diarrhea is a common condition in dogs, but studies evaluating the enteropathogens involved specifically in adult dogs are scarce. In the present study, stool samples from 45 adult dogs with bloody diarrhea were evaluated for the four enteric organisms mainly reported in these cases: canine parvovirus type 2 (CPV-2), Clostridioides difficile, Clostridium perfringens, and Salmonella spp. In addition, the samples were also tested for coronavirus, rotavirus, Giardia spp., and Escherichia coli pathotypes to provide a better understanding of possible co-occurrence. Vaccination status, diet, and clinical outcome were also obtained when available. CPV-2b was identified in 17 dogs (37.8%), being the most frequent cause of bloody diarrhea, including completely vaccinated adult dogs. Toxigenic C. difficile and C. perfringens netF+ were detected in 6 (13.3%) and 5 (11.1%) dogs, in some cases in a co-occurrence with other enteric organisms. Three fatal cases of salmonellosis were identified in dogs fed a raw meat-based diet, raising the risks associated with this increasing practice. Veterinary School. Universidade Federal de Minas Gerais (UFMG) UNESP-São Paulo State University - Department of Animal Production and Preventive Veterinary Medicine Universidade Federal do Rio Grande do Sul (UFRGS) Clínica Veterinária MedVet Clínica Veterinária VetMaster UNESP-São Paulo State University - Department of Animal Production and Preventive Veterinary Medicine CAPES: CAPES – Prêmio CAPES 2015 - 0774/2017 CNPq: CNPq - 406402/2018-3 FAPEMIG: FAPEMIG - APQ-00524-17

Nocardia asiatica causing pyogranulomatous pleuropneumonia is reported for the first time in a dog coinfected with canine morbillivirus (CM), diagnosed based on epidemiological, clinical, haematological, images, microbiological, histopathological, polymerase chain reaction and hsp65 gene sequencing findings. The immunosuppression of CM probably favoured the opportunistic behaviour of N. asiatica. Despite the therapeutic measures, the animal died, mainly due to respiratory distress. The association of methods to improve early diagnosis, therapy procedures and prognosis of canine nocardiosis is discussed, as well as the close relationship between pets and their owners, which may favour the transmission of pathogens such as Nocardia from pets‐to‐humans, which poses an emerging public health issue., Nocardia asiatica causing pyogranulomatous pleuropneumonia were report for the first time in a dog coinfected with canine morbillivirus.

Made available in DSpace on 2021-06-25T10:44:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Intraperitoneal ceftriaxone administration in healthy horses results in high and prolonged peritoneal concentrations. Recent findings suggest that intraperitoneal ceftriaxone might increase survival rates in horses affected by peritonitis. The present study aimed to evaluate plasma and peritoneal concentrations of ceftriaxone after intraperitoneal administration in horses with septic peritonitis. Twenty-six horses presenting clinical, laboratorial, and sonographic findings compatible with the disease were included. All horses received daily intraperitoneal ceftriaxone (25 mg/kg bwt) in addition or not with other antibiotics and support therapies. High-performance liquid chromatography was used to determine plasma and peritoneal ceftriaxone concentrations before and after 12 and 24 hours of ceftriaxone administration. Mean plasma concentrations 12 and 24 hours after administration were, respectively, 1.84 ± 0.43 and 0.37 ± 0.07 μg/mL, and mean peritoneal concentrations were 5.7 ± 2.84 and 0.42 ± 0.13 μg/mL. Ceftriaxone concentration was lower in comparison with previous studies in healthy horses and presented under the minimal inhibitory concentration for enterobacteria (≤1 μg/mL) and for gram-positive isolates (≤0.5 μg/mL) at 24 hours. The variation of the results obtained between healthy horses and with septic peritonitis demonstrated that pharmacokinetics/dynamics are different between these patients and suggests the use of an interval of dose of 12 hours. Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) Department of Natural Active Principles and Toxicology School of Pharmaceutical Sciences Univ. Estadual Paulista (Unesp) Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) Department of Veterinary Clinics School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) Department of Natural Active Principles and Toxicology School of Pharmaceutical Sciences Univ. Estadual Paulista (Unesp) Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) Department of Veterinary Clinics School of Veterinary Medicine and Animal Science Univ. Estadual Paulista (Unesp) FAPESP: # 2016/08713-8

Developers often use the C preprocessor to handle variability and portability. However, many researchers and practitioners criticize the use of preprocessor directives because of their negative effect on code understanding, maintainability, and error proneness. This negative effect may lead to configuration-related code weaknesses, which appear only when we enable or disable certain configuration options. A weakness is a type of mistake in software that, in proper conditions, could contribute to the introduction of vulnerabilities within that software. Configuration-related code weaknesses may be harder to detect and fix than weaknesses that appear in all configurations, because variability increases complexity. To address this problem, we propose a sampling-based white-box technique to detect configuration-related weaknesses in configurable systems. To evaluate our technique, we performed an empirical study with 24 popular highly configurable systems that make heavy use of the C preprocessor, such as Apache Httpd and Libssh. Using our technique, we detected 57 configuration-related weaknesses in 16 systems. In total, we found occurrences of the following five kinds of weaknesses: 30 memory leaks, 10 uninitialized variables, 9 null pointer dereferences, 6 resource leaks, and 2 buffer overflows. The corpus of these weaknesses is a valuable source to better support further research on configuration-related code weaknesses.

Code smells typically indicate poor design implementation and choices that may degrade software quality. Hence, they need to be carefully detected to avoid such poor design. In this context, some studies try to understand the impact of code smells on the software quality, while others propose rules or machine learning-based techniques to detect code smells. However, none of those studies or techniques focus on analyzing code snippets that are really harmful to software quality. This paper presents a study to understand and classify code harmfulness. We analyze harmfulness in terms of CLEAN, SMELLY, BUGGY, and HARMFUL code. By HARMFUL CODE, we define a SMELLY code element having one or more bugs reported. These bugs may have been fixed or not. Thus, the incidence of HARMFUL CODE may represent a increased risk of introducing new defects and/or design problems during its fixing. We perform our study with 22 smell types, 803 versions of 13 open-source projects, 40,340 bugs and 132,219 code smells. The results show that even though we have a high number of code smells, only 0.07% of those smells are harmful. The Abstract Function Call From Constructor is the smell type more related to HARMFUL CODE. To cross-validate our results, we also perform a survey with 60 developers. Most of them (98%) consider code smells harmful to the software, and 85% of those developers believe that code smells detection tools are important. But, those developers are not concerned about selecting tools that are able to detect HARMFUL CODE. We also evaluate machine learning techniques to classify code harmfulness: they reach the effectiveness of at least 97% to classify HARMFUL CODE. While the Random Forest is effective in classifying both SMELLY and HARMFUL CODE, the Gaussian Naive Bayes is the less effective technique. Our results also suggest that both software and developers' metrics are important to classify HARMFUL CODE.

Program refactoring consists of code changes applied to improve the internal structure of a program and, as a consequence, its comprehensibility. Recent studies indicate that developers often perform composite refactorings, i.e., a set of two or more interrelated single refactorings. Recent studies also recommend certain patterns of composite refactorings to fully remove poor code structures, i.e, code smells, thus further improving the program comprehension. However, other recent studies report that composite refactorings often fail to fully remove code smells. Given their failure to achieve this purpose, these composite refactorings are considered incomplete, i.e, they are not able to entirely remove a smelly structure. Unfortunately, there is no study providing an in-depth analysis of the incompleteness nature of many composites and their possibly partial impact on improving, maybe decreasing, internal quality attributes. This paper identifies the most common forms of incomplete composites, and their effect on quality attributes, such as coupling and cohesion, which are known to have an impact on program comprehension. We analyzed 353 incomplete composite refactorings in 5 software projects, two common code smells (Feature Envy and God Class), and four internal quality attributes. Our results reveal that incomplete composite refactorings with at least one Extract Method are often (71%) applied without Move Methods on smelly classes. We have also found that most incomplete composite refactorings (58%) tended to at least maintain the internal structural quality of smelly classes, thereby not causing more harm to program comprehension. We also discuss the implications of our findings to the research and practice of composite refactoring.

Made available in DSpace on 2021-06-25T10:33:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-22 Background Intraperitoneal administration of ceftriaxone maintains therapeutic abdominal concentrations for 24 hours in healthy horses. Therefore, it is a possible treatment for septic peritonitis. The aim of this study was to evaluate the efficacy of ceftriaxone as an adjuvant treatment in horses with septic peritonitis. Methods Twenty-six horses with clinical signs, sonography and/or laboratory findings of septic peritonitis were included. Peritoneal fluid was collected for microbiological culture and in vitro microbial sensitivity profile assessment. Daily intraperitoneal administration of ceftriaxone (25 mg/kg) was initiated with supportive and systemic antimicrobial treatment. The animals were divided into three groups: group 1 - gastrointestinal tract injuries and abdominal surgery (excluding perforations/ruptures); group 2 - not related to changes in the gastrointestinal tract; group 3 - secondary to intestinal rupture and/or faeces contamination. Results The mean success rate of the treatment was 77 per cent (20/26 animals), with success rates of 84.6 per cent in group 1; 87.5 per cent, group 2; and 40 per cent, group 3. Conclusions This is the first study to report adjuvant intraperitoneal treatment ceftriaxone for septic peritonitis in horses and indicates that this treatment can successfully treat septic peritonitis in horses. Department of Veterinary Surgery and Anaesthesiology Univ Estadual Paulista (UNESP) School of Veterinary Medicine and Animal Science (FMVZ) Department of Veterinary Clinics Univ Estadual Paulista (UNESP) School of Veterinary Medicine and Animal Science (FMVZ) Department of Veterinary Hygiene and Public Health Univ Estadual Paulista (UNESP) School of Veterinary Medicine and Animal Science (FMVZ) Department of Veterinary Surgery and Anaesthesiology Univ Estadual Paulista (UNESP) School of Veterinary Medicine and Animal Science (FMVZ) Department of Veterinary Clinics Univ Estadual Paulista (UNESP) School of Veterinary Medicine and Animal Science (FMVZ) Department of Veterinary Hygiene and Public Health Univ Estadual Paulista (UNESP) School of Veterinary Medicine and Animal Science (FMVZ)

Summary This report describes a fatal case of a pet dog with major enteric signs owned by a family that has experienced cases of pulmonary tuberculosis (TB) in the household. Clinical and epidemiological aspects, imaging data, microbiological, haematological and histopathological examinations were assessed to diagnosis of disease. gyrB-RFLP, spoligotyping and MIRU-VNTR allowed molecular detection of M. tuberculosis strain from S family. The resazurin microtiter assay indicated that all isolates were resistant to isoniazid, ethambutol, ciprofloxacin, ofloxacin, streptomycin and amikacin. The public health concerns related to canine tuberculosis and risk of the dissemination by pets of M. tuberculosis pre-multidrug-resistant (PMD) to isoniazid, ethambutol and other first-line drugs used in human therapy of TB are discussed. We believe this to be the first report of PMD M. tuberculosis infection in a dog presenting mainly enteric manifestation, confirmed as S lineage by molecular methods, owned by a family in which TB has spread in the household for generations.

Made available in DSpace on 2021-07-14T10:51:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-02-01. Added 1 bitstream(s) on 2021-07-14T11:39:50Z : No. of bitstreams: 1 S1808-16572017000100310.pdf: 1155174 bytes, checksum: c76d6bb77200a5082ccd09bca6df7577 (MD5) A dermatofilose bovina é uma dermatite caracterizada por lesões focais ou localizadas com aspecto de “pincel” e, ocasionalmente, como lesão cutânea disseminada. Relata-se o caso de uma fêmea bovina de um ano de idade, que foi atendida apresentando história de lesões cutâneas crônicas imediatamente após um período de alta pluviosidade. Ao exame clínico, lesões serosas a purulentas, com hiperqueratose, coalescentes, não pruriginosas, ressecadas, de coloração amarelada à acinzentada foram observadas distribuídas de modo generalizado pelo animal. A remoção das crostas revelou áreas ulceradas ou hemorrágicas, com crostas irregulares e elevadas semelhantes a “pincel”. O diagnóstico microbiológico possibilitou a identificação do micro-organismo Dermatophilus congolensis. Apesar das lesões disseminadas e crônicas, a cura do animal foi obtida com tratamento parenteral usando oxitetraciclina de longa duração, baseado em teste in vitro de sensibilidade microbiana modificado. O presente relato ressalta o sucesso no tratamento de caso incomum de lesões generalizadas de dermatofilose bovina com respaldo de teste in vitro de sensibilidade modificado, bem como a necessidade do uso responsável de antimicrobianos em animais de produção. Bovine dermatophilosis is a dermatitis characterized by typical focal or localized lesions with “paintbrush” aspect and occasionally as disseminated cutaneous disease. We report the case of a one-year-old Nelore female with history of chronic cutaneous disseminated lesions that appeared immediately after a rainfall period. Serous to purulent exudates, hair with tufted appearance, hyperkeratotic, non-pruritic, hardened, yellowish to brown, and coalescent crusty lesions were observed distributed all over its body. Removal of the crusts revealed ulcerated or hemorrhagic areas, with irregular elevated crusts like “paintbrush”. Microbiological diagnosis enabled the identification of a microorganism, the Dermatophilus congolensis. Despite disseminated and chronic lesions, we obtained a successful therapy with parenteral therapy using long-acting tetracycline based on modified in vitro disk diffusion test. The present report highlights success therapy in uncommon generalized bovine dermatophilosis with selection of first-choice drugs based on modified in vitro susceptibility test, and need of responsible use of antimicrobials in livestock. Universidade Estadual Paulista “Júlio de Mesquita Filho” Universidade Estadual Paulista “Júlio de Mesquita Filho”

Made available in DSpace on 2018-12-11T17:20:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-02-01. Added 1 bitstream(s) on 2019-10-09T18:31:43Z : No. of bitstreams: 1 S0100-736X2018000200285.pdf: 637186 bytes, checksum: ec7d078217f3640931007f17821b9320 (MD5) Tetanus is characterized by high case fatality rates in horses. Comprehensive case series studies involving equine tetanus from different geographic areas enable the evaluation of prognosis, efficacy of treatment, and control measures. We retrospectively investigated some selected epidemiological data (breed, age, gender, use of the horses, history of vaccination, seasonality, presence of wound/history of surgical procedures, clinical outcomes) and main clinical aspects (clinical signs, incubation period, length of hospitalization, and period between onset signs and hospitalization) in 70 cases of equine tetanus over 1990-2015, with emphasis in the association between these data and the clinical outcomes. High mortality rate (72.9%) was observed in this study. Forty (57.1%) horses presented history of wounds or surgical procedures related with tetanus, represented mainly by lesions in the hind limbs (42.5%), front limbs (15.0%), umbilical infections (7.1%), castration (4.3%), and face wounds (4.3%). Hyperesthesia, limb spasticity, cervical stiffness, tetanic spasms, and restriction of jaw movement were the main consistent clinical signs. Besides no statistical association, all the horses with umbilical infections, wounds in face, prolonged recumbency, sweating, dysphagia/aphagia died, and together with delay between onset of first clinical signs and prompt veterinary assistance ( < 5 days) were considered indicative of poor prognosis; whereas there was a significant association (p=0.001) between survival and length of hospitalization > 7 days, seemed as an evidence of good prognosis. The high mortality rate of tetanus, even in horses under specific treatment, highlight the need for early diagnosis, prompt veterinary assistance, and establishment of prophylatic measures in equine farms. Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista 'Júlio de Mesquita Filho' (Unesp), Cx. Postal 560 Faculdade de Tecnologia (FATEC), Av. José ítalo Bacchi s/n. Departamento de Biotecnologia e Bioprocessos Faculdade de Agronomia Unesp Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista 'Júlio de Mesquita Filho' (Unesp), Cx. Postal 560 Departamento de Biotecnologia e Bioprocessos Faculdade de Agronomia Unesp

Brucellosis remains as a public health concern worldwide. In domestic animals, the disease is characterized by reproductive disorders in male and female. Besides extensive use of serological tests and recent development of molecular biology techniques, microbiological culture of Brucella species is yet considered a “gold standard” method for diagnosis. Here, semen of 335 bovine bulls was subjected simultaneously to microbiological culture in Brucella agar, Farrell media, and CITA media to evaluate comparatively the best selective media for isolation of Brucella sp. Among all 335 samples, B. abortus B19 strain was isolated from semen of five (1.49%) bulls using the three selective media. However, Farrell media was considered the best selective media for microbiological diagnosis, because of allowed isolation of B. abortus B19 strain from bull semen without bacterial commensal or fungal contamination of plates. Key words: Brucella agar, Farrell media, CITA media, bovine bulls, semen.

A horse with recurrent airway disease was presented with dyspnea, mucopurulent bilateral nasal discharge and abnormal lung sounds. Microbiological culture, ...

Made available in DSpace on 2015-02-02T12:39:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-11-01Bitstream added on 2015-02-02T13:07:50Z : No. of bitstreams: 1 S0103-84782014001102035.pdf: 557333 bytes, checksum: 7e3feb42e2cd676f3d80668df2dbe757 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Relata-se caso incomum de osteomielite mandibular deformante em vaca, causada por Trueperella (Arcanobacterium) pyogenes, na face ventro-lateral da porção caudal do ramo direito da mandíbula. A punção aspirativa de fragmento da lesão permitiu a caracterização citológica, o isolamento microbiano e identificação de T. pyogenes. Exame radiográfico mostrou acentuada reação periodontal na mandíbula direita, com predomínio de áreas líticas e destruição da cortical óssea. Apesar da instituição do tratamento baseado no teste de sensibilidade microbiana in vitro, foi recomendada a eutanásia, em virtude da piora progressiva do estado geral do animal. No exame post-mortem foram observados múltiplos abscessos na lesão que, histologicamente, foi caracterizada como piogranuloma. A colheita de material da região mandibular afetada, após a necropsia, resultou no reisolamento microbiológico de T. pyogenes This study reports an unusual case of deforming mandibular osteomyelitis in a cow caused by Trueperella (Arcanobacterium) pyogenes, on the face of the ventrolateral caudal portion of the right branch of the mandible. Fragment aspired of lesion by fine needle allowed cytological characterization, isolation and identification of T. pyogenes. Radiographic examination showed marked periosteal reaction in the right mandible, numerous lytic areas and cortical bone destruction. Despite of treatment based on in vitro antimicrobial sensitivity test, it was recommended the euthanasia due to progressive worsening of the cow's condition. Multiple abscesses were observed in the mandibular region at necropsy. Pyogranuloma was characterized in histological exam. Sampled material collected from the lesion after necropsy resulted in microbiological reisolation of T. pyogenes Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia UNESP Departamento de Cirurgia e Anestesiologia Veterinária FMVZ UNESP Departamento de Reprodução Animal e Radiologia Veterinária FMVZ Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia UNESP Departamento de Cirurgia e Anestesiologia Veterinária FMVZ UNESP Departamento de Reprodução Animal e Radiologia Veterinária FMVZ

The aim of the present study was to compare the test performances of three commercial enzyme immunoassays (EIAs) against the toxigenic culture using the cytotoxicity assay as the gold standard. All EIAs showed >78% sensitivity, and the lowest specificity was 92.6%. These results suggest that EIAs could be useful for the diagnosis of Clostridium difficile infection in foals.

Made available in DSpace on 2013-09-27T14:54:12Z (GMT). No. of bitstreams: 1 WOS000314513600028.pdf: 594657 bytes, checksum: 3b48dcbc94a3826215ff6e6552f098f5 (MD5) Previous issue date: 2012-01-01 Made available in DSpace on 2013-09-30T18:26:17Z (GMT). No. of bitstreams: 1 WOS000314513600028.pdf: 594657 bytes, checksum: 3b48dcbc94a3826215ff6e6552f098f5 (MD5) Previous issue date: 2012-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:38:20Z No. of bitstreams: 1 WOS000314513600028.pdf: 594657 bytes, checksum: 3b48dcbc94a3826215ff6e6552f098f5 (MD5) Made available in DSpace on 2014-05-20T13:38:20Z (GMT). No. of bitstreams: 1 WOS000314513600028.pdf: 594657 bytes, checksum: 3b48dcbc94a3826215ff6e6552f098f5 (MD5) Previous issue date: 2012-01-01 This study investigated the occurrence of equine arteritis virus antibodies using neutralization test in 1,400 horses from Campinas and macro metropolitan paulista mesoregions located at São Paulo State, between 2007 and 2008. Eighty (5.7%) samples showed antibodies to virus (titers from 4 to 4,096). Among the 42 cities, 15 (35.7%) presented at least one seropositive animal to equine arteritis virus. of the 238 farms analyzed 41 showed at least one seropositive animal. The occurrence was higher in sport horses like jumping horses, Quarter horses and was similar for female and male. The rate of seropositive was higher in animals older than 24 months of age. These results suggest the virus circulation among horse population in studied farms, and the need for continuing epidemiological surveillance studies. Univ Estadual Paulista, UNESP, FMVZ, Botucatu, SP, Brazil Inst Biol, São Paulo, Brazil Univ Estadual Paulista, UNESP, FMB, Botucatu, SP, Brazil Univ Estadual Paulista, UNESP, FMVZ, Botucatu, SP, Brazil Univ Estadual Paulista, UNESP, FMB, Botucatu, SP, Brazil

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:56:38Z No. of bitstreams: 1 S0102-09352012000400036.pdf: 344111 bytes, checksum: 04b17e11433b38d41eff9e22f7dceaf0 (MD5) Made available in DSpace on 2013-08-22T18:56:38Z (GMT). No. of bitstreams: 1 S0102-09352012000400036.pdf: 344111 bytes, checksum: 04b17e11433b38d41eff9e22f7dceaf0 (MD5) Previous issue date: 2012-08-01 Made available in DSpace on 2013-09-30T19:50:46Z (GMT). No. of bitstreams: 2 S0102-09352012000400036.pdf: 344111 bytes, checksum: 04b17e11433b38d41eff9e22f7dceaf0 (MD5) S0102-09352012000400036.pdf.txt: 16494 bytes, checksum: b75f4851581aeac7e34b71433bf6ba2f (MD5) Previous issue date: 2012-08-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:39:49Z No. of bitstreams: 2 S0102-09352012000400036.pdf: 344111 bytes, checksum: 04b17e11433b38d41eff9e22f7dceaf0 (MD5) S0102-09352012000400036.pdf.txt: 16494 bytes, checksum: b75f4851581aeac7e34b71433bf6ba2f (MD5) Made available in DSpace on 2014-05-20T13:39:49Z (GMT). No. of bitstreams: 2 S0102-09352012000400036.pdf: 344111 bytes, checksum: 04b17e11433b38d41eff9e22f7dceaf0 (MD5) S0102-09352012000400036.pdf.txt: 16494 bytes, checksum: b75f4851581aeac7e34b71433bf6ba2f (MD5) Previous issue date: 2012-08-01 A retrospective study of major clinical and epidemiological findings was performed in 11 cases of tetanus observed in eight sheep and three goats from 1998 to 2008. The cases occurred predominantly in animals aging from five days to two years, in winter season, with history of recent wounds or surgical procedures. Muscle rigidity and muscular tremors (72.0%), tachycardia/dyspnea (54.5%), change of placement of ears (45.4%), protusion of the third eyelid (27.2%), hypersensitivity to external stimulus (18.1%), and elevation of ears (18.1%) were the most common clinical signs. UNESP Faculdade de Medicina Veterinária e Zootecnia UNESP Faculdade de Medicina Veterinária e Zootecnia

The aim of this study was to compare the prevalence of virulence genes in 158 Escherichia coli strains isolated from 51 clinical cases of UTIs, 52 of pyometra and from 55 fecal samples from healthy dogs by PCR. papC was found in 12 (23.5%) strains isolated from UTIs, 19 (36.5%) from pyometra and 10 (18.2%) from feces. papGII was observed in 3 (5.8%) strains from pyometra, and papGIII in 10 (19.6%) from UTIs, 15 (28.8%) from pyometra and 9 (16.4%) from feces. sfaS was detected in 22 (43.1%) strains from UTIs, 24 (46.1%) from pyometra and 19 (34.5%) from feces. hlyA was observed in 17 (33.3%) strains from UTIs, 18 (34.6%) from pyometra and 7 (12.7%) from feces, while cnf-1 was detected in 11 (21.6%) from UTIs, 21 (40.4%) from pyometra and 9 (16.4%) from feces. iucD was observed in 12 (23.5%) strains from UTIs, 9 (17.3%) from pyometra and 1 (1.8%) from feces. usp was found 17 (33.3%) isolates from UTIs and 36 (69.9%) from pyometra.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:56:05Z No. of bitstreams: 1 S0102-09352008000200031.pdf: 199936 bytes, checksum: 9dcfd9480c09ffecebb4f743fef16ea4 (MD5) Made available in DSpace on 2013-08-22T18:56:05Z (GMT). No. of bitstreams: 1 S0102-09352008000200031.pdf: 199936 bytes, checksum: 9dcfd9480c09ffecebb4f743fef16ea4 (MD5) Previous issue date: 2008-04-01 Made available in DSpace on 2013-09-30T19:49:36Z (GMT). No. of bitstreams: 2 S0102-09352008000200031.pdf: 199936 bytes, checksum: 9dcfd9480c09ffecebb4f743fef16ea4 (MD5) S0102-09352008000200031.pdf.txt: 12684 bytes, checksum: bb933daa6147470698b4d06f3f14ef5f (MD5) Previous issue date: 2008-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:39:49Z No. of bitstreams: 2 S0102-09352008000200031.pdf: 199936 bytes, checksum: 9dcfd9480c09ffecebb4f743fef16ea4 (MD5) S0102-09352008000200031.pdf.txt: 12684 bytes, checksum: bb933daa6147470698b4d06f3f14ef5f (MD5) Made available in DSpace on 2014-05-20T13:39:49Z (GMT). No. of bitstreams: 2 S0102-09352008000200031.pdf: 199936 bytes, checksum: 9dcfd9480c09ffecebb4f743fef16ea4 (MD5) S0102-09352008000200031.pdf.txt: 12684 bytes, checksum: bb933daa6147470698b4d06f3f14ef5f (MD5) Previous issue date: 2008-04-01 Relata-se a ocorrência de graves sintomas de mastite hiperaguda em vaca, causada por Klebsiella pneumoniae, na terceira semana de lactação. Descrevem-se aspectos epidemiológicos, sintomas clínicos, procedimentos de diagnóstico microbiológico, conduta terapêutica e medidas de controle. UNESP Faculdade de Medicina Veterinária e Zootecnia UNESP Faculdade de Medicina Veterinária e Zootecnia

Made available in DSpace on 2015-10-21T13:09:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-05-01. Added 1 bitstream(s) on 2015-10-22T09:53:08Z : No. of bitstreams: 1 S0100-736X2015000500417.pdf: 861121 bytes, checksum: ac9adc3c00e3f606e6465134126eb605 (MD5) O leite informal é reconhecido como o produto comercializado sem inspeção. Avaliou-se a qualidade microbiológica, constituintes nutricionais e presença de substâncias inibidoras do crescimento bacteriano em 100 amostras informais de leite de vacas, comercializadas na região sudoeste do estado de São Paulo, Brasil. Verificou-se que 77% da Contagem de Células Somáticas (CCS) e 86% da Contagem Bacteriana Total (CBT) das amostras estavam em desacordo com os valores máximos exigidos pela Instrução Normativa 62 do MAPA. Identificaram-se teores em desacordo para sólidos totais (36%), proteína (23%), gordura (38%), extrato seco desengordurado (43%) e nitrogênio uréico (73%). Em 59% das amostras detectou-se a presença de resíduos de inibidores do crescimento bacteriano. Isolaram-se 240 linhagens de micro-organismos com predomínio de enterobactérias, estreptococos, estafilococos e fungos. O leite informal é comercializado na região sem as mínimas condições de higiene e oferece riscos a saúde pública, além de representar sério problema sócio-econômico para região. The informal milk is recognized as the product marketed without inspection. We evaluated the microbiological quality, nutritional ingredients and substances inhibiting bacterial growth in 100 informal samples from cows milk, marketed in the southwest region of the state of Sao Paulo, Brazil. It was found that 77% of Somatic Cell Count (SCC) and 86% of Total Bacterial Count (TBC) of the samples were at odds with the maximum values required by Instruction Rules 62 of the Ministry of Agriculture and Livestock Supply. It was identified to solids contents in disagreement (36%), protein (23%), fat (38%), nonfat dry extract (43%) and 73% urea nitrogen. In 59% of the samples was detected residues of the bacterial growth inhibitors. Were isolated 240 strains of micro-organisms with a prevalence of enterobacteria, streptococci, staphylococci and fungi. The informal milk is marketed in the region without the minimum hygienic conditions and present risks to public health, and represents serious socio-economic problem for the region. Univ Estadual Paulista, Dept Higiene Vet &Saude Publ, Fac Med Vet &Zootecnia FMVZ, UNESP, BR-18618970 Botucatu, SP, Brazil Univ Estadual Feira de Santana, Dept Ciencias Biol DCBIO, BR-44036900 Feira De Santana, BA, Brazil Univ Oeste Paulista Unoeste, Dept Vet Prevent Med, BR-19067175 Presidente Prudente, SP, Brazil Univ Estadual Campinas, Dept Genet Evolucao &Bioagentes, Inst Biol, UNICAMP, BR-13083970 Campinas, SP, Brazil Univ Estadual Ctr Oeste Parana Unicentro, Curso Med Vet, BR-85040080 Guarapuava, PR, Brazil Univ Estadual Paulista, Dept Higiene Vet & Saude Publ, Fac Med Vet &Zootecnia FMVZ, UNESP, BR-18618970 Botucatu, SP, Brazil

Black carbon, charcoal and mercury fluxes were measured from sediment cores taken in an artificial water dam in an intense land use change area in the Alta Floresta district in the Brazilian Amazon, in order to characterize the differences in the evolution of human occupation patterns in the region during the last 18 years. A positive correlation between the black carbon and charcoal particle fluxes and the evolution of the Brazilian gross domestic production (GDP) was observed. Mercury fluxes showed a positive correlation with gold production and exhibited a distinct evolution pattern when compared to in relation to the forest fires indicators and Brazilian GDP. The fluxes of forest fires markers showed an increase in deforestation activities in the region after 1993. Mercury deposition showed a substantial decrease after 1994. The patterns of distribution in both forest fires tracers and gold mining tracers indicate substitution of the regional economic model. It also marked different antropogenic impact type in the ecosystem.

Relata-se polissinovite auto-imune por Rhodococcus equi em potro, secundária à severa enterocolite. Ao exame clínico, constatou-se debilidade do estado geral, diarréia e aumento das articulações nos quatro membros. Exames microbiológicos e citológicos de aspirado articular revelaram sinovite asséptica. Em virtude da debilidade progressiva do estado geral, recomendou-se a eutanásia. À necropsia constatou-se enterocolite, linfadenite mesentérica, congestão pulmonar, foco de abscesso em lobo pulmonar e espessamento das cápsulas articulares. Ressalta-se a ocorrência não-usual de polissinovite auto-imune por R. equi em potro, decorrente da deposição de imunocomplexos articulares, secundária à severa enterite e linfadenite mesentérica.

Antibacterial activity of violacein against Staphylococcus aureus isolated from Bovine Mastitis

Most clinical cases of osteomyelitis in dogs involve infectious agents, especially bacteria and fungi. The characterization of these microorganisms may aid in the prevention and treatment of disease.The aim of this study was to evaluate retrospectively microbiological cultures and in vitro antimicrobial susceptibility profile of isolates from 52 cases of bacterial osteomyelitis in long bones of dogs over 2000-2013. In 78% of the cases injuries were caused by a motor vehicle accident, but there were a few cases of dog bites (17%) and ascending infection due to pododermatitis (5%).The isolated microorganisms were identified based on conventional phenotypic methods. In vitro disk diffusion test was performed using 30 different antimicrobials.The isolates were obtained from femur (28%), humerus (16%), tibia (31%), and radius/ulna (25%). Among 52 cases, culture was positive in 88% of cases. Thirteen genus of different species of microorganisms were isolated. The most common microorganisms isolated were Staphylococcus spp. and Escherichia coli followed by Streptococcus spp., enteric bacteria, Corynebacterium sp. and anaerobic bacteria. In 42% of cases cultures were mixed. The most effective drugs against isolated bacteria were amoxicillin and clavulanate potassium (79%) followed by ceftriaxone (69%). High-resistance rates were documented against azithromycin (80%), penicillin (59%), and clindamycin (59%).The present study highlights diverse etiologic agents in cases of infectious bacterial osteomyelitis, with predominance of Staphylococcus genus, and reinforces the importance of obtaining cultures and susceptibility profiles given the high rates of antimicrobial resistance.

Made available in DSpace on 2015-02-02T12:39:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-10-01Bitstream added on 2015-02-02T13:08:09Z : No. of bitstreams: 1 S0102-09352014000501339.pdf: 333900 bytes, checksum: 744df5f93290a4f3177d81f8e4f0260f (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) A Criptococose é uma importante doença infecciosa fúngica, causada por uma levedura do gênero Cryptococcus, que acomete diferentes espécies inclusive o homem. Há poucos relatos na literatura sobre a criptococose pulmonar em cães. O presente trabalho relata um caso de criptococose em um cão apresentando alterações respiratórias, especialmente dispneia. O diagnóstico foi realizado por meio da citologia aspirativa, após toracotomia exploratória, sendo observado um quadro de pleuris grave. O paciente foi tratado durante 90 dias com itraconazol e apresentou, ao final do tratamento, a remissão completa dos sintomas relatados. Cryptococcosis is a major infectious disease caused by a yeast from the Cryptococcus genre which affects different species including humans. There are few reports related to pulmonary cryptococcosis in dogs. A case of Cryptococcosis in a dog showing dyspnea was described. The diagnosis was done by aspiration cytology after exploratory thoracotomy, and a picture of severe pleurisy was observed. The diagnosis was done by needle aspiration cytology after thoracotomy. Severe pleuris frame was observed. Treatment with itraconazole was performed for 90 days, and at the end of the treatment the dog presented complete remission of reported symptoms. UNESP FMVZ UNESP FMVZ

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:55:37Z No. of bitstreams: 1 S0102-09352004000300021.pdf: 150424 bytes, checksum: 01431bce085c5c12535592b3b4af0cd4 (MD5) Made available in DSpace on 2013-08-22T18:55:37Z (GMT). No. of bitstreams: 1 S0102-09352004000300021.pdf: 150424 bytes, checksum: 01431bce085c5c12535592b3b4af0cd4 (MD5) Previous issue date: 2004-06-01 Made available in DSpace on 2013-09-30T19:48:39Z (GMT). No. of bitstreams: 2 S0102-09352004000300021.pdf: 150424 bytes, checksum: 01431bce085c5c12535592b3b4af0cd4 (MD5) S0102-09352004000300021.pdf.txt: 8764 bytes, checksum: c1ea958ee9b66622b1ef5258be4c8ed4 (MD5) Previous issue date: 2004-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:12:53Z No. of bitstreams: 2 S0102-09352004000300021.pdf: 150424 bytes, checksum: 01431bce085c5c12535592b3b4af0cd4 (MD5) S0102-09352004000300021.pdf.txt: 8764 bytes, checksum: c1ea958ee9b66622b1ef5258be4c8ed4 (MD5) Made available in DSpace on 2014-05-20T15:12:53Z (GMT). No. of bitstreams: 2 S0102-09352004000300021.pdf: 150424 bytes, checksum: 01431bce085c5c12535592b3b4af0cd4 (MD5) S0102-09352004000300021.pdf.txt: 8764 bytes, checksum: c1ea958ee9b66622b1ef5258be4c8ed4 (MD5) Previous issue date: 2004-06-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Clinic-epidemiological data in canine infection by Hepatozoon canis were reported. Apatia, anorexia, prostaction, weight loss and muscle pain were the most-common clinical recognized signs. Blood smear was used as diagnosis method for the detection of H. canis gamonts in the neutrophils. Anaemia, leucocytosis, monocytosis and lymphopenia were observed in the haematological exams. Imidocarb dipropionate associated with tetracyclines and sulfamethoxasole-trimethoprim were used in therapy. USP FMVZ Depto. Medicina Veterinária Preventiva e Saúde Animal CNPq UNESP FMVZ Depto. Higiene Veterinária e Saúde Pública UNESP FMVZ Depto. Higiene Veterinária e Saúde Pública

Clostridial myositis is an acute, generally fatal toxemia that is considered to be rare in pet animals. The present report describes an unusual canine clostridial myositis that was diagnosed by a new multiplex-PCR (mPCR) designed for simultaneous identification of Clostridium sordellii, Clostridium septicum, Clostridium perfringens type A, Clostridium chauvoei, and Clostridium novyi type A. A ten-month-old male Rottweiler dog, that had displayed lameness and swelling of the left limb for 12 h, was admitted to a veterinary hospital. The animal was weak, dyspneic and hyperthermic, and a clinical examination indicated the presence of gas and edema in the limb. Despite emergency treatment, the animal died in only a few minutes. Samples of muscular tissue from the necrotic area were aseptically collected and plated onto defibrinated sheep blood agar (5%) in anaerobic conditions. Colonies suggestive of Clostridium spp. were submitted to testing by multiplex-PCR. Impression smears of the tissues, visualized with Gram and also with panoptic stains, revealed long rod-shaped organisms, and specimens also tested positive using the fluorescent antibody technique (FAT). The FAT and mPCR tests enabled a diagnosis of C. septicum myonecrosis in the dog.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:56:36Z No. of bitstreams: 1 S0102-09352012000100032.pdf: 123319 bytes, checksum: 2eec616549457bf971468e6f387ae7e0 (MD5) Made available in DSpace on 2013-08-22T18:56:36Z (GMT). No. of bitstreams: 1 S0102-09352012000100032.pdf: 123319 bytes, checksum: 2eec616549457bf971468e6f387ae7e0 (MD5) Previous issue date: 2012-02-01 Made available in DSpace on 2013-09-30T19:50:41Z (GMT). No. of bitstreams: 2 S0102-09352012000100032.pdf: 123319 bytes, checksum: 2eec616549457bf971468e6f387ae7e0 (MD5) S0102-09352012000100032.pdf.txt: 12924 bytes, checksum: d172bef8774f077c028dca7085802505 (MD5) Previous issue date: 2012-02-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:39:53Z No. of bitstreams: 2 S0102-09352012000100032.pdf: 123319 bytes, checksum: 2eec616549457bf971468e6f387ae7e0 (MD5) S0102-09352012000100032.pdf.txt: 12924 bytes, checksum: d172bef8774f077c028dca7085802505 (MD5) Made available in DSpace on 2014-05-20T13:39:53Z (GMT). No. of bitstreams: 2 S0102-09352012000100032.pdf: 123319 bytes, checksum: 2eec616549457bf971468e6f387ae7e0 (MD5) S0102-09352012000100032.pdf.txt: 12924 bytes, checksum: d172bef8774f077c028dca7085802505 (MD5) Previous issue date: 2012-02-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) O presente estudo relata a ocorrência de co-infecção entre o vírus da cinomose canina (CDV) e Toxoplama gondii em cães com sinais neurológicos. Amostras de soro e tecido nervoso (pos-mortem) de 21 cães, suspeitos de cinomose canina foram analisadas pela Reação de Imunofluorecência indireta (RIFI) para pesquisa de anticorpos contra T. gondii e N. caninum e por RT-PCR para CDV. Dezessete (80,9%) cães foram positivos para o CDV pela RT-PCR e 8 (38,1%) foram positivos para anticorpos contra T. gondii. Sete cães (41,1%) apresentaram-se positivos para ambos agentes, caracterizando processo de co-infecção. Somente 1 (4,7%) cão foi soropositivo para N. caninum (RIFI=100), entretanto este mesmo animal foi positivo para T. gondii (RIFI=4096) e para CDV (RT-PCR). Universidade Federal de Mato Grosso Faculdade de Agronomia, Medicina Veterinária e Zootecnia Universidade de Cuiabá Faculdade de Medicina Veterinária Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual de Londrina (UEL) Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista Faculdade de Medicina Veterinária e Zootecnia

Made available in DSpace on 2013-09-27T14:54:16Z (GMT). No. of bitstreams: 1 WOS000314514200033.pdf: 738858 bytes, checksum: f4e34138e6bfa61855f68ed706bdf5b6 (MD5) Previous issue date: 2012-01-01 Made available in DSpace on 2013-09-30T18:26:44Z (GMT). No. of bitstreams: 1 WOS000314514200033.pdf: 738858 bytes, checksum: f4e34138e6bfa61855f68ed706bdf5b6 (MD5) Previous issue date: 2012-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:40:08Z No. of bitstreams: 1 WOS000314514200033.pdf: 738858 bytes, checksum: f4e34138e6bfa61855f68ed706bdf5b6 (MD5) Made available in DSpace on 2014-05-20T13:40:08Z (GMT). No. of bitstreams: 1 WOS000314514200033.pdf: 738858 bytes, checksum: f4e34138e6bfa61855f68ed706bdf5b6 (MD5) Previous issue date: 2012-01-01 A retrospective study of etiology, antimicrobial susceptibility profile and multiple drug resistance, and major epidemiological aspects were investigated in 616 cases of canine otitis. Staphylococcus beta hemolitic (26.27%), Malassezia pachydermatis (12.35%), and Pseudomonas aeruginosa (8.8%) were the most common microorganisms identified. The isolates were susceptible mainly to norfloxacin (89.62%), gentamicin (83.25%), and ofloxacin (80.16%). High occurrence of resistance of isolates was observed to neomicin (30.84%) and cephalexin (27.63%). Multiple drug resistance to three or more and five or more of antimicrobials tested was observed in 34.9% and 15.5% of isolates, respectively. The cases of canine otitis occurred predominantly in first years of age, in mixed breeds animals, at autumn season. The presence of itch, bad smell, and secretion in ear conduct were the major signs observed at clinical examination. FMVZ UNESP, DHVSP, Dept Higiene Vet & Saúde Publ, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil FMVZ UNESP, DHVSP, Botucatu, SP, Brazil FMVZ UNESP, SASPVSA, Botucatu, SP, Brazil FMVZ UNESP, DHVSP, Tecn Lab Diagnost Microbiol, Botucatu, SP, Brazil FMVZ UNESP, DHVSP, Dept Higiene Vet & Saúde Publ, Fac Med Vet & Zootecnia, Botucatu, SP, Brazil FMVZ UNESP, DHVSP, Botucatu, SP, Brazil FMVZ UNESP, SASPVSA, Botucatu, SP, Brazil FMVZ UNESP, DHVSP, Tecn Lab Diagnost Microbiol, Botucatu, SP, Brazil

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:56:30Z No. of bitstreams: 1 S0102-09352011000300027.pdf: 114642 bytes, checksum: 7cd57ed89e1286ad4c8dd7b236edfe59 (MD5) Made available in DSpace on 2013-08-22T18:56:30Z (GMT). No. of bitstreams: 1 S0102-09352011000300027.pdf: 114642 bytes, checksum: 7cd57ed89e1286ad4c8dd7b236edfe59 (MD5) Previous issue date: 2011-06-01 Made available in DSpace on 2013-09-30T19:50:30Z (GMT). No. of bitstreams: 2 S0102-09352011000300027.pdf: 114642 bytes, checksum: 7cd57ed89e1286ad4c8dd7b236edfe59 (MD5) S0102-09352011000300027.pdf.txt: 17111 bytes, checksum: df80140e663d966f8dcf3a8686104037 (MD5) Previous issue date: 2011-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:39:52Z No. of bitstreams: 2 S0102-09352011000300027.pdf: 114642 bytes, checksum: 7cd57ed89e1286ad4c8dd7b236edfe59 (MD5) S0102-09352011000300027.pdf.txt: 17111 bytes, checksum: df80140e663d966f8dcf3a8686104037 (MD5) Made available in DSpace on 2014-05-20T13:39:52Z (GMT). No. of bitstreams: 2 S0102-09352011000300027.pdf: 114642 bytes, checksum: 7cd57ed89e1286ad4c8dd7b236edfe59 (MD5) S0102-09352011000300027.pdf.txt: 17111 bytes, checksum: df80140e663d966f8dcf3a8686104037 (MD5) Previous issue date: 2011-06-01 An uncommon outbreak of mastitis caused by Arcanobacterium pyogenes in 26 cows is reported. The epidemiological findings, clinical signs, microbiological exams, somatic cell count, in vitro susceptibility profile of strains, efficacy of intramammary treatment and control measures were discussed. Florfenicol (96.2%), cefoperazona (92.3%), cefaloxin (84.6%) and ceftiofur (84.6%) were the most effective antimicrobials, and neomicin (27.0%) and enrofloxacin (17.4%) the least effective antimicrobials. UNESP Faculdade de Medicina Veterinária e Zootecnia UNESP Faculdade de Medicina Veterinária e Zootecnia

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:44:57Z No. of bitstreams: 1 S1984-29612009000300010.pdf: 485148 bytes, checksum: 0fbb9c0212951b0a0c2c2b31ade3e99c (MD5) Made available in DSpace on 2013-08-22T18:44:58Z (GMT). No. of bitstreams: 1 S1984-29612009000300010.pdf: 485148 bytes, checksum: 0fbb9c0212951b0a0c2c2b31ade3e99c (MD5) Previous issue date: 2009-09-01 Made available in DSpace on 2013-09-30T18:24:21Z (GMT). No. of bitstreams: 2 S1984-29612009000300010.pdf: 485148 bytes, checksum: 0fbb9c0212951b0a0c2c2b31ade3e99c (MD5) S1984-29612009000300010.pdf.txt: 27191 bytes, checksum: 31da3bdb60091596ca871753d9908550 (MD5) Previous issue date: 2009-09-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:38:34Z No. of bitstreams: 2 S1984-29612009000300010.pdf: 485148 bytes, checksum: 0fbb9c0212951b0a0c2c2b31ade3e99c (MD5) S1984-29612009000300010.pdf.txt: 27191 bytes, checksum: 31da3bdb60091596ca871753d9908550 (MD5) Made available in DSpace on 2014-05-20T13:38:34Z (GMT). No. of bitstreams: 2 S1984-29612009000300010.pdf: 485148 bytes, checksum: 0fbb9c0212951b0a0c2c2b31ade3e99c (MD5) S1984-29612009000300010.pdf.txt: 27191 bytes, checksum: 31da3bdb60091596ca871753d9908550 (MD5) Previous issue date: 2009-09-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) O presente estudo investigou a etiologia da erliquiose monocítica canina em 70 cães atendidos no Hospital Veterinário da Universidade Estadual Paulista, na cidade de Botucatu, durante 2001 e 2002. Os cães foram avaliados segundo achados clínicos, epidemiológicos e laboratoriais e pela amplificação parcial e sequenciamento do gene dsb de Ehrlichia. DNA de Ehrlichia canis foi amplificado e sequenciado em 28 (40,0%) cães. Observou-se maior frequência deanimais positivos com idade até 12 meses (P < 0,05). Diarreia, apatia e anorexia foram os sinais clínicos mais frequentes observados em 55,2% (P = 0,05), 47,0% (P > 0,05) e 42,2% (P > 0,05) dos cães PCR positivos, respectivamente. Vinte e cinco cães com anemia ( 0,05) frente à infecção por E. canis. Todos os 28 cães positivos na PCR apresentaram trombocitopenia ( 0.05), and 42.4% (P > 0.05) of the PCR-positive dogs, respectively. Twenty-five anemic ( 0.05). All 28 PCR-positive dogs showed thrombocytopenia (

Made available in DSpace on 2013-09-27T14:54:17Z (GMT). No. of bitstreams: 1 WOS000265076400022.pdf: 314646 bytes, checksum: bfdf3ee53ae3a8940a8d3fd618299d84 (MD5) Previous issue date: 2009-01-01 Made available in DSpace on 2013-09-30T18:26:42Z (GMT). No. of bitstreams: 1 WOS000265076400022.pdf: 314646 bytes, checksum: bfdf3ee53ae3a8940a8d3fd618299d84 (MD5) Previous issue date: 2009-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:39:55Z No. of bitstreams: 1 WOS000265076400022.pdf: 314646 bytes, checksum: bfdf3ee53ae3a8940a8d3fd618299d84 (MD5) Made available in DSpace on 2014-05-20T13:39:55Z (GMT). No. of bitstreams: 1 WOS000265076400022.pdf: 314646 bytes, checksum: bfdf3ee53ae3a8940a8d3fd618299d84 (MD5) Previous issue date: 2009-01-01 Canine papillomatosis is an infectious viral disease characterized by oral, cutaneous or ocular papillomas, usually benign. The treatment is indicated in animals, with multiple tumors that produce pharyngeal obstruction, and problems, of eating or for aesthetic reasons. Different treatment protocols have been proposed, including surgical excision, cryosurgery, electro surgery, autogenous or recombinant vaccines, imunomodulators drugs, systemic and intralesional chemotherapy. In this study were reviewed the more important aspects of canine oral papillomatosis. In the 12 studied animals, the papillomas were observed predominantly in mouth, gum and palate regions, in puppies until 12 months, presenting combined infection with ehrlichiosis. The treatment using Propionibacterium acnes and/or autogenous vaccine showed efficacy in eight dogs (66.7%). UNESP, FMVZ, Dept Hygiene Vet Saúde Publ, Botucatu, SP, Brazil UNESP, FMVZ, Dept Hygiene Vet Saúde Publ, Botucatu, SP, Brazil

Domestic pigs are considered to be important sources of Toxoplasma gondii infection for humans. Due to the increased consumption of wild boar meat in Brazil, this species may also be an important source of the parasite. The objective of the present study was to detect T. gondii infection in 306 blood samples collected from wild boars bred in the state of Sao Paulo, Brazil. Samples were analyzed using the modified agglutination test (MAT), and 14 (4.5%) of them yielded positive results. Modern breeding techniques may have contributed to the low frequency of infection observed. Results indicated that wild boars were exposed to T. gondii and that the consumption of this kind of meat may represent a source of infection for humans.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:05:23Z No. of bitstreams: 1 S0036-46652008000300009.pdf: 2173007 bytes, checksum: 18bec24e8efa83481c4063277e743716 (MD5) Made available in DSpace on 2013-08-22T19:05:23Z (GMT). No. of bitstreams: 1 S0036-46652008000300009.pdf: 2173007 bytes, checksum: 18bec24e8efa83481c4063277e743716 (MD5) Previous issue date: 2008-06-01 Made available in DSpace on 2013-09-30T18:24:36Z (GMT). No. of bitstreams: 2 S0036-46652008000300009.pdf: 2173007 bytes, checksum: 18bec24e8efa83481c4063277e743716 (MD5) S0036-46652008000300009.pdf.txt: 50950 bytes, checksum: 248cda6ae2b97d98f6938881a6f9498a (MD5) Previous issue date: 2008-06-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:38:42Z No. of bitstreams: 2 S0036-46652008000300009.pdf: 2173007 bytes, checksum: 18bec24e8efa83481c4063277e743716 (MD5) S0036-46652008000300009.pdf.txt: 50950 bytes, checksum: 248cda6ae2b97d98f6938881a6f9498a (MD5) Made available in DSpace on 2014-05-20T13:38:42Z (GMT). No. of bitstreams: 2 S0036-46652008000300009.pdf: 2173007 bytes, checksum: 18bec24e8efa83481c4063277e743716 (MD5) S0036-46652008000300009.pdf.txt: 50950 bytes, checksum: 248cda6ae2b97d98f6938881a6f9498a (MD5) Previous issue date: 2008-06-01 A caracterização fenotípica, perfil de sensibilidade aos antimicrobianos e aspectos clínico-epidemiológicos foram avaliados em 28 linhagens de Nocardia isoladas de 19 casos de mastite, oito lesões tegumentares e um caso de pneumonia em cão. Foram utilizados no diagnóstico métodos microbiológicos, bioquímicos, citológicos e microscopia eletrônica de varredura. Nocardia asteroides tipo IV, N. otitidiscaviarum,N. nova (tipo III) e N. farcinica (tipo V) foram isoladas do leite de vacas com mastite, de material de lavado transtraqueal e de lesões cutâneas de cães. Nocardiose mamária bovina foi diagnosticada predominantemente sob a forma clínica, em propriedades com precárias condições de higiene na pré e pós-ordenha, e inadequado procedimento de terapia intramamária. Nocardiose canina foi diagnosticada comumente em animais co-infectados com o vírus da cinomose. Sulfametoxazole/trimetoprim (92,8%), amicacina (92,8%) e ceftiofur (92,8%) foram os antimicrobianos mais efetivos frente às linhagens de Nocardia. Resistência múltipla a três ou mais e cinco ou mais antimicrobianos foram observadas, respectivamente, em dez (35,7%) e três (10,7%) linhagens, notadamente frente à cloxacilina, cefoperazona e ampicilina. A caracterização de espécies (tipo), aspectos clínico-epidemiológicos, diagnóstico, resistência múltipla aos antimicrobianos e reflexos em saúde pública de linhagens de Nocardia isoladas de bovinos e cães no Brasil foram discutidos. Foi destacada a similaridade entre as espécies de Nocardia isoladas de animais e do homem, e a primeira descrição no Brasil de N. otitidiscaviarum na etiologia da mastite bovina. Phenotypic characteristics, antimicrobial susceptibility profile, and clinical-epidemiological features of 28 Nocardia strains isolated from 19 cases of bovine mastitis, eight cutaneous-subcutaneous lesions and one case of pneumonia in dogs were evaluated. Microbiological, biochemical, cytological and scanning electron microscopy methods were used in diagnosis. Nocardia asteroides type IV, Nocardia otitidiscaviarum,Nocardia nova (type III) and Nocardia farcinica (type V) were isolated from bovine milk, bronchial lavage and/or cutaneous-subcutaneous abscesses in dogs. Nocardial bovine mastitis was diagnosed predominantly in clinical cases, in dairy herds with poor environmental hygienic conditions between milking and inappropriate intramammary therapy. Canine nocardiosis was observed commonly in animals co-infected with distemper virus. Sulphamethoxazole-trimethoprim (92.8%), amikacin (92.8%) and ceftiofur (92.8%) were the most effective drugs in 28 isolates. Multiple drug resistance to three or more and five or more antimicrobials was observed in ten (35.7%) and three (10.7%) strains, respectively, predominantly with use of cloxaxillin, cefoperazone and ampicillin. The species (type) classification, clinical-epidemiological characteristics, diagnosis, multiple-drug resistance and public health considerations in Nocardia strains isolated from cattle and dogs in Brazil are discussed, with special reference to report of bovine mastitis by N. otitidiscaviarum by first time in Brazil and the similarity between Nocardia species isolated from human and animal origin. UNESP Faculdade de Medicina Veterinária e Zootecnia Departamento de Higiene Veterinária e Saúde Pública UNESP FMVZ Universidade do Estado do Rio de Janeiro Faculdade de Ciências Médicas UNESP FMVZ Departamento de Higiene Veterinária e Saúde Pública UNESP Faculdade de Medicina Veterinária e Zootecnia Departamento de Higiene Veterinária e Saúde Pública UNESP FMVZ UNESP FMVZ Departamento de Higiene Veterinária e Saúde Pública

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:55:34Z No. of bitstreams: 0 Made available in DSpace on 2013-08-22T18:55:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-02-01 Made available in DSpace on 2013-09-30T19:48:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-02-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:12:52Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T15:12:52Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-02-01 Fistulous wither secretions from three horses were tested by the plate agglutination (PAT), tube agglutination (SAT), buffered plate-Rose Bengal (RBPT) and 2-mercaptoethanol (2-ME) tests, comparatively with standard agglutination tests. In the modified tests, titers were increased in the PAT, SAT and 2-ME tests and positive reaction was observed in RBPT. Brucella abortus was isolated from the secretion of fistulous withers collected from one animal. These results suggest that the modified tests may be used as alternative tests to diagnose brucellosis in horses with fistulous withers. UNESP Faculdade de Medicina Veterinária e Zootecnia UNESP Faculdade de Medicina Veterinária e Zootecnia

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:55:30Z No. of bitstreams: 0 Made available in DSpace on 2013-08-22T18:55:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2001-10-01 Made available in DSpace on 2013-09-30T19:48:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2001-10-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:39:49Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T13:39:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2001-10-01 Propionibacterium acnes as immunostimulant in oral canine papillomatosis treatment in 16 animals was studied. Regression of the pappiloma started being observed after the second aplication, with complete resolution in all dogs after the sixth aplication. These results suggest the use of P. acnes as an alternative in oral canine papillomatosis therapy. UNESP Faculdade de Medicina Veterinária e Zootecnia UNESP Faculdade de Medicina Veterinária e Zootecnia

Relatam-se quatro casos de toxoplasmose em caes, evidenciando-se a sintomatologia nervosa indistinguivel daquela causada pela cinomose de forma isolada e mostrando a ocorrencia concomitante das duas enfermidades. Sugere-se que os dados de anamnese, como habitos de carnivorismo e contato com gatos, aliados a sinais clinicos como linfadenopatia, pneumonia, secrecao ocular purulenta e disturbios neurologicos, favorecam a suspeita clinica de toxoplasmose. Propoe-se um tratamento, preventivo para a toxoplasmose, nos caes com cinomose, baseado nas condicoes de diagnostico disponiveis pelo clinico.