1. Kidney-intrinsic factors determine the severity of ischemia/reperfusion injury in a mouse model of delayed graft function
- Author
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Charlie Lin, Zheng Zhang, Feibo Zheng, Yashpal S. Kanwar, Shulin Han, Jiao Jing Wang, Daniele Procissi, Deyu Fang, Longhui Qiu, Xingqiang Lai, Michael Abecassis, Edward B. Thorp, Lin Li, Zhuoli Zhang, and Xin Yi Yeap
- Subjects
0301 basic medicine ,Pathology ,Kidney Disease ,030232 urology & nephrology ,Kidney ,ischemia/reperfusion injury ,Cold Ischemia Time ,Mice ,0302 clinical medicine ,Ischemia ,2.1 Biological and endogenous factors ,Aetiology ,innate immunity ,Kidney transplantation ,Inbred BALB C ,Mice, Inbred BALB C ,Mice, Inbred C3H ,Graft Survival ,Urology & Nephrology ,Inbred C3H ,surgical procedures, operative ,medicine.anatomical_structure ,Nephrology ,Reperfusion Injury ,donor factors ,medicine.medical_specialty ,mouse model ,Clinical Sciences ,Renal and urogenital ,Delayed Graft Function ,kidney transplantation ,Article ,03 medical and health sciences ,Downregulation and upregulation ,medicine ,Animals ,Transplantation ,business.industry ,Animal ,Endoplasmic reticulum ,Organ Transplantation ,medicine.disease ,Disease Models, Animal ,030104 developmental biology ,TRIF ,Disease Models ,business ,Reperfusion injury - Abstract
Delayed graft function due to transplant ischemia/reperfusion injury adversely affects up to 50% of deceased-donor kidney transplant recipients. However, key factors contributing to the severity of ischemia/reperfusion injury remain unclear. Here, using a clinically relevant mouse model of delayed graft function, we demonstrated that donor genetic background and kidney-intrinsic MyD88/Trif-dependent innate immunity were key determinants of delayed graft function. Functional deterioration of kidney grafts directly corresponded with the duration of cold ischemia time. The graft dysfunction became irreversible after cold ischemia time exceeded six hours. When cold ischemia time reached four hours, kidney grafts displayed histological features reflective of delayed graft function seen in clinical kidney transplantation. Notably, kidneys of B6 mice exhibited significantly more severe histological and functional impairment than kidneys of C3H or BALB/c mice, regardless of recipient strains or alloreactivities. Furthermore, allografts of B6 mice also showed an upregulation of IL-6, neutrophil gelatinase-associated lipocalin, and endoplasmic reticulum stress genes, as well as an increased influx of host neutrophils and memory CD8 T-cells. In contrast, donor MyD88/Trif deficiency inhibited neutrophil influx and decreased the expression of IL-6 and endoplasmic reticulum stress genes, along with improved graft function and prolonged allograft survival. Thus, kidney-intrinsic factors involving genetic characteristics and innate immunity serve as critical determinants of the severity of delayed graft function. This preclinical murine model allows for further investigations of the mechanisms underlying delayed graft function.
- Published
- 2020