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Development of novel antimicrobials capable of providing long-lasting disinfection on surfaces requires the implementation of new standardized methods to support claims recognized by regulatory authorities. Surrogates for viral pathogens are often desired in order for BSL-2 labs to conduct these new efficacy tests safely and efficiently, however, the knowledge of surrogate suitability for these tests is limited. Here, for the first time, we determine the resistance profile of the bacteriophage Phi6 to quaternary ammonium compounds (QACs) in a variety of test conditions. Additionally, we show that Phi6 can be used to demonstrate the long-lasting virucidal efficacy of a novel antimicrobial, Actizone™ F5, and that the Phi6 is more resistant to QACs following the UK standard long-lasting disinfection test BSI PAS2424:2014 than Vaccinia virus, which is the marker strain for claims of activity against enveloped viruses in Europe. Surface stability as well as benefits and limitations of Phi6 use relative to other enveloped viruses for antimicrobial testing is also discussed.