Search

PurposeLong non-coding RNAs (lncRNAs) play an important role in the occurrence and development of bladder cancer, but the underlying molecular mechanisms remain largely unknown. In this study, we found that LINC00467 was significantly highly expressed in bladder cancer through bioinformatic analysis. The present study aimed to explore the role of LINC00467 in bladder cancer and its possible underlying molecular mechanisms.MethodsThe expression of LINC00467 was obtained from GEO (GSE31189), the TCGA database, and qRT-PCR. The role of LINC00467 in bladder cancer was assessed both in vitro and in vivo. RIP, RNA pulldown, and CO-IP were used to demonstrate the potential mechanism by which LINC00467 regulates the progression of bladder cancer.ResultsThrough the analysis of GEO (GSE133624) and the TCGA database, it was found that LINC00467 was highly expressed in bladder cancer tissues and that the expression of LINC00467 was significantly negatively correlated with patient prognosis. Cell and animal experiments suggest that LINC00467 promotes the proliferation and invasion of bladder cancer cells. On the one hand, LINC00467 can directly bind to NF-kb-p65 mRNA to stabilize its expression. On the other hand, LINC00467 can directly bind to NF-kb-p65 to promote its translocation into the nucleus to activate the NF-κB signaling pathway, which promotes the progression of bladder cancer.ConclusionsLINC00467 is highly expressed in bladder cancer and can promote the progression of bladder cancer by regulating the NF-κB signaling pathway. Therefore, targeting LINC00467 is very likely to provide a new strategy for the treatment of bladder cancer and for improving patient prognosis.

In traditional centralized application construction and data storage modes, the application servers and the users are not in equal position in user information management. The application server can store and abuse the user's information under the circumstances that the users are not aware, and the user cannot revoke the authorization from the application server. Therefore, this paper proposes an identity management system combined with blockchain technology, which can return the management right of user information to users. This system adopts a three-layer architecture composed of blockchain layer, virtual chain layer and storage layer, and encapsulates all functions in each layer. It has good flexibility, and is easy to add new technologies, with good extensibility. The blockchain records the user's information state to realize the consensus and integrity of the user's data. The virtual chain layer is responsible for the main logical functions and encapsulates the user's request into a blockchain transaction, maintaining a good portability. The storage layer is responsible for the actual file storage and is responsible for file routing, backup, and query. Comprehensively speaking, based on the above three layers' architecture, the system implements the user's privacy granularity control, the user file's external storage, and combines the zero-knowledge proof to update the user's private key. In view of the application environment of this system, the access time delay and efficiency of the P2P storage system are simulated. It solves the problems of centralized application construction and data storage mode under which users cannot fully control personal information and privacy protection.

Early diagnosis is essential for improving the prognosis and survival of patients with hepatocellular carcinoma (HCC). This study aims to explore the clinical value of lipoprotein subfractions in the diagnosis of hepatitis B virus (HBV)-related HCC. Lipoprotein subfractions were detected by 1H-NMR spectroscopy, and the pattern-recognition method and binary logistic regression were performed to classify distinct serum profiles and construct prediction models for HCC diagnosis. Differentially expressed proteins associated with lipid metabolism were detected by LC-MS/MS, and the potential prognostic significance of the mRNA expression was evaluated by Kaplan–Meier survival analysis. The diagnostic panel constructed from the serum particle number of very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), and low-density lipoprotein (LDL-1~LDL-6) achieved higher accuracy for the diagnosis of HBV-related HCC and HBV-related benign liver disease (LD) than that constructed from serum alpha-fetoprotein (AFP) alone in the training set (AUC: 0.850 vs. AUC: 0.831) and validation set (AUC: 0.926 vs. AUC: 0.833). Furthermore, the panel achieved good diagnostic performance in distinguishing AFP-negative HCC from AFP-negative LD (AUC: 0.773). We also found that lipoprotein lipase (LPL) transcript levels showed a significant increase in cancerous tissue and that high expression was significantly positively correlated with the poor prognosis of patients. Our research provides new insight for the development of diagnostic biomarkers for HCC, and abnormal lipid metabolism and LPL-mediated abnormal serum lipoprotein metabolism may be important factors in promoting HCC development.

The aim of this study was to investigate the information-processing changes during an auditory oddball task among healthy controls (HCs), convalescent bipolar depression (BPC), and bipolar depression (BPD) patients. Thirty patients with BPD, 27 patients with BPC, and 30 HC were recruited in this study. The participants' performance (reaction time and accuracy) as well as P300 amplitude and latency during the tests were analyzed statistically. BPD and BPC showed poor task performance compared with HC. BPD had lower P300 amplitude and delayed P300 latency in the frontal, central, and parietal areas than HC. BPC had lower P300 amplitude, but no difference in P300 latency compared with the HC group. Further, P300 latency was significantly delayed over the right relative to the left region. In conclusion, bipolar patients had P300 abnormalities and deficits in cognitive processing of stimulus, and may be present even when emotional symptoms were relieved.

Background: The Tower of London (TOL) task is one of the most commonly used tests for evaluating executive functions, and can indicate planning and problem-solving abilities. The aim of this study was to evaluate hemodynamic changes between the task period and rest period in patients with bipolar depression during the TOL task and the verbal fluency task (VFT) using near-infrared spectroscopy (NIRS). Methods: Forty-three patients with bipolar depression and 32 healthy controls (HCs) matched for sex, age, handedness, and years of education were enrolled in this study. All participants were aged between 16 and 50. All patients in our study were taking medications such as antidepressants, antipsychotics and mood stabilizers at the time of measurement. Changes in oxygenated hemoglobin (oxy-Hb) levels in frontal areas during the TOL task and VFT were evaluated using a 41-channel NIRS system. Results: During the TOL task, the patients with bipolar depression exhibited significantly smaller changes in the bilateral dorsal-lateral prefrontal cortex (DLPFC) than the HCs. During the VFT task, the patients with bipolar depression exhibited significantly smaller changes in the right ventrolateral prefrontal cortex (VLPFC), the right DLPFC and both the right and left prefrontal cortex (PFC) than the HCs. Limitations: Our sample size was small, and the effects of medication cannot be excluded. Conclusions: These results indicate that planning and problem solving dysfunction is related to the impairment of the prefrontal cortex in patients with bipolar depression, and NIRS can be used to assess planning and problem solving abilities, which are essential to daily life in patients with bipolar disorder.

According as the composing and working theory of the taxing UAV, aim at the controlling for the takeoff and landing, the monitor software for the takeoff and landing is proposed. This software is based on Microsoft Visual C++6.0 and the virtual instrument. The software can real-time display flight attitude, track, parameters and the tracking divation. It showed the feasibility and necessarily of the softswar for the monitor and control system for the takeoff and landing for taxiing UAV.