1. Compositional alterations of gut microbiota in children with primary nephrotic syndrome after initial therapy
- Author
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Ying Wu, Helen K. W. Law, Yulin Kang, Dan Feng, Wei Qu, Guang-Hua Zhu, and Wen-Yan Huang
- Subjects
0301 basic medicine ,DNA, Bacterial ,Male ,medicine.medical_specialty ,China ,Nephrotic Syndrome ,Firmicutes ,Gut microbiota ,Gut flora ,lcsh:RC870-923 ,T-Lymphocytes, Regulatory ,digestive system ,Microbiology ,Primary nephrotic syndrome ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,RNA, Ribosomal, 16S ,Outcome Assessment, Health Care ,medicine ,Humans ,Age of Onset ,Child ,Glucocorticoids ,Children ,Feces ,biology ,Bacteria ,business.industry ,digestive, oral, and skin physiology ,Bacteroidetes ,biology.organism_classification ,medicine.disease ,lcsh:Diseases of the genitourinary system. Urology ,Gastrointestinal Microbiome ,Gastrointestinal Tract ,030104 developmental biology ,Nephrology ,030220 oncology & carcinogenesis ,Isoflavonoid biosynthesis ,Dysbiosis ,Female ,Proteobacteria ,business ,Research Article - Abstract
Background Primary nephrotic syndrome (PNS) is a common glomerular disease in children. T cell dysfunction plays a crucial role in the pathogenesis of PNS. Moreover, dysbiosis of gut microbiota contributes to immunological disorders. Whether the initial therapy of PNS affects gut microbiota remains an important question. Our study investigated compositional changes of gut microbiota after initial therapy. Methods Fecal samples of 20 children with PNS were collected before and after 4-week initial therapy. Total bacteria DNA were extracted and the V3-V4 regions of bacteria 16S ribosomal RNA gene were sequenced. The composition of gut microbiota before and after initial therapy was analyzed by bioinformatics methods. The function of altered gut microbiota was predicted with PICRUSt method. Results The richness and diversity of gut microbiota were similar before and after 4-week initial therapy. Gut microbiota at the phylum level was dominated by four phyla including Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria, but the increased relative abundance after initial therapy was found in Deinococcus-Thermus and Acidobacteria. At the genus level, the increased abundance of gut microbiota after initial therapy was observed in short chain fat acids (SCFA)-producing bacteria including Romboutsia, Stomatobaculum and Cloacibacillus (p Conclusions Initial therapy of PNS increased SCFA-producing gut microbiota, but might diminish selenocompound metabolism, isoflavonoid biosynthesis and phosphatidylinositol signaling system in children.
- Published
- 2019