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In recent years, the development of adjunctive therapeutic hyperthermia for cancer therapy has received considerable attention. However, the mechanisms underlying hyperthermia resistance are still poorly understood. In this study, we investigated the roles of cold-inducible RNA binding protein (Cirbp) in regulating hyperthermia resistance and underlying mechanisms in nasopharyngeal carcinoma (NPC). Our results firstly revealed that hyperthermia significantly attenuated the stemness property of NPC cells, while combination treatment of hyperthermia and oridonin dramatically increased the killing effect on NPC cells and cancer stem cell (CSC)-like population. Moreover, hyperthermia substantially improved the sensitivity of radiation-resistant NPC cells and CSC-like cells to radiotherapy. Hyperthermia noticeably suppressed Cirbp expression in NPC cells and xenograft tumor tissues. Furthermore, Cirbp inhibition remarkably boosted anti-tumor-killing activity of hyperthermia against NPC cells and CSC-like cells, whereas ectopic expression of Cirbp compromised tumor-killing effect of hyperthermia on these cells, indicating that Cirbp overexpression induces hyperthermia resistance. ThermomiR-377–3p improved the sensitivity of NPC cells and cancer stem-like cells to hyperthermia in vitro by directly suppressing Cirbp expression. More importantly, our results displayed the significantly boosted sensitization of tumor xenografts to hyperthermia by Cirbp silencing in vivo, but ectopic expression of Cirbp nearly completely counteracted hyperthermia-mediated tumor cell-killing effect against tumor xenografts in vivo. Mechanistically, Cirbp silencing-induced inhibition of DNA damage repair by inactivating ATM-Chk2 and ATR-Chk1 pathways, decrease in stemness and increase in cell death contributed to hyperthermic sensitization; conversely, Cirbp overexpression-induced promotion of DNA damage repair, increase in stemness and decrease in cell apoptosis contributed to hyperthermia resistance. Taken together, these findings reveal a previously unrecognized role for Cirbp in positively regulating hyperthermia resistance and suggest that thermomiR-377–3p and its target gene Cirbp represent promising targets for therapeutic hyperthermia.

Objective To investigate the role of HELQ in chemo-resistance of epithelial ovarian carcinoma (EOC), which is a critical factor of patients' prognosis. Methods Immunohistochemistry, survival analysis of our 87 EOC patients and bioinformatics analysis of The Cancer Genome Atlas (TCGA) datasets (Nature, 2011) disclosed the clinical importance of HELQ expression. Quantitative reverse transcription polymerase chain reaction (qRT-PCR), and Western Blot analyses of EOC tissue were used to confirm it. Ectopic overexpression and RNA interference knockdown of HELQ were carried out in OVCAR3 and A2780 cell lines, respectively, to determine the effect of altered HELQ expression on cellular response to cisplatin by CCK8 assay. The DNA repair capacity of these cells was evaluated by using host-cell reactivation assay. Western Blot analyses were carried out to determine the effect of HLEQ on the DNA repair genes by using cells with altered HELQ expression. Results HELQ expression associates with response of EOC patients to platinum-based chemotherapy and their overall survival (OS), disease free survival (DFS). HELQ overexpression or knockdown, respectively, increased and decreased the cellular resistance to cisplatin, DNA repair activity, and expression of DNA repair proteins of Nucleotide excision repair (NER) pathway. Conclusions HELQ plays an important role in regulating the expression of DNA repair proteins NER pathway which, in turn, contributes to cellular response to cisplatin and patients' response to platinum-based chemotherapy. Our results demonstrated that HELQ could serve as a novel indicator for chemo-resistance of EOC, which can predict the prognosis of the disease.

Shanghai main power grid is operating in 500 kV ring network and with voltage-grading & district-dividing structure, and its subzone power grids have their own frequency characteristics. The paper has used district-dividing single equivalent model to check the parameters of components in BPA transient simulation program, and the original under frequency load shedding (UFLS) scheme was improved. Then the frequency dynamic characteristics of isolated subzone grid is analyzed, so does the adaptability and frequency safety of power grid under the new UFLS scheme in the condition of peak load in the summer of 2011.

Power transmission is a key link in power system. As the increase of power supply, the transmission capacity of the lines should be enlarged too. In the developed area, the right-of-way for transmission line is hard to be obtained. And converting the existing HVAC overhead transmission lines using HVDC technology could enhance the transmission capability. There are three possible plans for different HVAC transmission lines: single-pole HVDC, bi-pole HVDC and tri-pole HVDC.

Lift makes a vehicle in air and thrust makes advancing. The lift of flapping wing air vehicle is composed of two main parts, flapping lift and advancing lift. The advancing lift of flapping-wing is similar as of fixed-wing, generated mainly by relative velocity and angle of attack. The flapping lift is owned only by flapping wing. The flapping lift is generated by asymmetry flapping motion manner of wings, asymmetry airfoil, and asymmetry folding in flapping cycle, accordingly leading-edge vortex and wake capture effect. The thrust is completely generated by flapping wing and the magnitude of thrust is mainly controlled by flapping frequency and flapping manner. The flapping motion is a thrust generator and lift enhancing manner. Flapping wing air vehicle will be one of the star members of man-made air vehicles.

This paper presents a computational fluid-structure dynamics coupling method of flexible wing. The computational fluid dynamics is solving RANS equations. The stiffness used in computational structure dynamics is tested by experiments. Then the aerodynamic performance of flexible wing is researched and compared with rigid wing. The results show that the lift and thrust generated by flexible wing are less than the rigid wing. Flexible wing can slower the variety of aerodynamic performance when the flight environment is changing. And flexible wing has bigger stalling angle.

The paper simulated and researched the straightening process of heavy rail by finite element software of ANSYS/LS-DYNA. The residual stress of the rail head, rail base, rail loin in the 7th deformation zone meets the real straightening condition in the straightening simulation. The calculation indicates: The residual stress of the rail head, rail base, rail loin in various deformation zones varies significantly like the variation of tensile-compression-tensile. Compared with the on-site rule, the residual stress of rail base decreased155 Mpa, this is in agreement with the values of practice.

Brain and leptomeningeal metastases are common in breast cancer patients and our current treatments are ineffective. Reovirus type 3 is a replication competent, naturally occurring virus that usurps the activated Ras-signaling pathway (or an element thereof) of tumor cells and lyses them but leaves normal cells relatively unaffected. In this study we evaluated reovirus as an experimental therapeutic in models of central nervous system (CNS) metastasis from breast cancer. We found all breast cancer cell lines tested were susceptible to reovirus, with > 50% of these cells lysed within 72 h of infection. In vivo neurotoxicity studies showed only mild local inflammation at the injection site and mild communicating hydrocephalus with neither diffuse encephalitis nor behavioral abnormalities at the therapeutically effective dose of reovirus (intracranial) (ie 10(7) plaque-forming units) or one dose level higher. In vivo, a single intratumoral administration of reovirus significantly reduced the size of tumors established from two human breast cancer cell lines and significantly prolonged survival. Intrathecal administration of reovirus also remarkably prolonged survival in an immunocompetent racine model of leptomeningeal metastases. These data suggest that the evaluation of reovirus as an experimental therapeutic for CNS metastases from breast cancer is warranted.