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Background:. Pathological changes of the cochlea and hearing loss have been well addressed in Waardenburg syndrome (WS). However, the vestibular organ malformation in WS is still largely unknown. In this study, the differentiation and development of vestibular sensory epithelium and vestibular function caused by SOX10 mutation, a critical gene induces WS, has been studied in minature pig model. Results: Degeneration of vestibular hair cells was found in this Sox10 mutation porcine model. Inner ear phenotype of the SOX10+/R109W miniature pigs showed cochlear abnormalities as well as saccular hypofunction. In the mutant pigs, no prominent dissimilarity was shown in the bone structure of the semicircular canals. However, the saccular membrane was collapsed and the infusion of stereocilia of the hair cells were observed. There was no dark cells in the uticules in th mutant pigs. The density of the utricular hair cells was also significantly lower in the mutant pigs compared to the wild type. Conclusions: Our study demonstrated that the SOX10 gene and melanocytes play important roles in the vestibular organ development. Sox10 mutation disrupts the KIT-DCT signaling pathway, affects the development of melanocytes and leads to vestibule morphogenesis.

The Ministry of education proposed to build a deep, difficult and challenging "golden course", which has become the goal of university curriculum construction. Although domestic university have made some attempts and researches on the online and offline hybrid curriculum construction, the research content does not directly combine the "golden course" with the "new engineering" construction content. In view of the various problems existing in the current research, this paper integrates the existing professional talent training scheme, curriculum system, teaching resources, teaching content and other aspects of local colleges, carries out theoretical research and practice, puts forward the research and practice of "golden course" construction of data science specialty under the background of new engineering, and integrates the concept of new engineering construction into on-line and off-line In the design of hybrid "golden course", the construction mode of "golden course" under the characteristics of new engineering specialty in local universities is gradually formed, so as to effectively increase the quality of curriculum teaching and personnel training.

ObjectiveTo describe and study the population statistics, hearing phenotype, and pathological changes of a porcine congenital single-sided deafness (CSSD) pedigree.MethodsClick auditory brainstem response (ABR), full-frequency ABR, and distortion product otoacoustic emission (DPOAE) were used to assess the hearing phenotype of the strain. Tympanogram was used to assess the middle ear function since birth. Celloidin embedding–hematoxylin–eosin (CE-HE) stain and scanning electron microscopy (SEM) were used to study the pathological changes of cochlear microstructures. Chi-square analysis was used to analyze the relation between hearing loss and other phenotypes.ResultsThe mating mood of CSSD with CSSD was most efficient in breeding-targeted CSSD phenotype (47.62%), and the prevalence of CSSD reached 46.67% till the fifth generation, where 42.22% were bilateral hearing loss (BHL) and 9.00% were normal hearing (NH) individuals. Hearing loss was proved to have no relation with coat color (P = 0.0841 > 0.05) and gender (P = 0.4621 > 0.05) by chi-square analysis. The deaf side of CSSD offspring in the fifth generation had no relation with that of their maternal parent (P = 0.2387 > 0.05). All individuals in this strain exhibited congenital severe to profound sensorineural hearing loss with no malformation and dysfunction of the middle ear. The good hearing ear of CSSD stayed stable over age. The deaf side of CSSD and BHL presented cochlear and saccular degeneration, and the hair cell exhibited malformation since birth and degenerated from the apex to base turn through time. The pathology in BHL cochlea progressed more rapidly than CSSD and till P30, the hair cell had been totally gone. The stria vascularis (SV) was normal since birth and degenerated through time and finally exhibited disorganization of three layers of cells.ConclusionThis inbred porcine strain exhibited high and stable prevalence of CSSD, which highly resembled human non-syndromic CSSD disease. This porcine model could be used to further explore the etiology of CSSD and serve as an ideal tool for the studies of the effects of single-sided hearing deprivation on neural, cognitive, and behavioral developments and the benefits brought by CI in CSSD individuals.

In order to improve the utilization rate of library seats, this paper analyzes the problems existing in the traditional library seat management system and provide a novel intelligent management system of library seat. The system framework of intelligent management system is established firstly, and different system functions are designed using C/s software and Java language and VB language and XML technology. The experiment results show the new method can effectively improve the comprehensive utilization efficiency of the library seat management system.

In the context of big data, information overload has emerged in various resources, especially the various learning resources available today. In order to effectively improve the recommendation accuracy of learning resources under the background of big data, firstly, the existing research on the recommendation system is analyzed. Then, based on the deep learning model, the deep learning model framework and the personalized learning recommendation framework are constructed. How to effectively obtain user and learning resource information. Finally, through the proposed framework model, the accuracy of personalized learning recommendations can be effectively improved.

With the continuous development of Internet technology, the phenomenon of information overload has appeared in the course learning resources in the network, which increases the difficulty for students to acquire learning resources. To this end, the distributed processing center is built in the grid environment, which enhances the efficiency of data collection and data mining. Combined with the deep learning training mode, the scoring matrix of students and curriculum resources can be effectively obtained, and the scoring matrix is similar. The calculation establishes a recommendation queue for personalized learning recommendation, and finally recommends through the information in the recommendation queue, which effectively improves the accuracy and efficiency of personalized network learning recommendation.

With the informationization of the development of party members in colleges and universities, the traditional management platform has been unable to meet the recommendation work of party members in colleges and universities. By analyzing the shortcomings of the existing party member management platform, this paper proposes a student party member development recommendation model based on the grid platform deep learning mode. Under the grid platform, the artificial intelligence method of deep learning training is used to set the development object. Different parameters and parameter weights are set, and repeated training makes the constructed model more optimized, thus obtaining the recommended queue, solving the manual operation mode of daily work, reducing the working time, improving the work efficiency, and making the whole process more intelligent.

BackgroundIncreasing number of hospitalized children with community acquired pneumonia (CAP) is co-detected withMycoplasma pneumoniae(Mp). The clinical characteristics and impact ofMpco-detected with other bacterial and/or viral pathogens remain poorly understood. The purpose of this study was to evaluate the demographic and clinical features of CAP children withMpmono-detection andMpco-detection.MethodsA total of 4148 hospitalized children with CAP were recruited from January to December 2017 at the Children’s Hospital of Hebei Province, affiliated to Hebei Medical University. A variety of respiratory viruses, bacteria andMpwere detected using multiple modalities. The demographic and clinical features of CAP children withMpmono-detection andMpco-detection were recorded and analyzed.ResultsAmong the 110 CAP children withMppositive, 42 (38.18%) of them were co-detected with at least one other pathogen. Co-detection was more common among children aged ≤3 years. No significant differences were found in most clinical symptoms, complications, underlying conditions and disease severity parameters among various etiological groups, with the following exceptions. First, prolonged duration of fever, lack of appetite and runny nose were more prevalent among CAP children withMp-virus co-detection. Second,Mp-virus (excludingHRV) co-detected patients were more likely to present with prolonged duration of fever. Third, patients co-detected withMp-bacteria were more likely to have abnormal blood gases. Additionally, CAP children withMp-HRVco-detection were significantly more likely to report severe runny nose compared to those withMpmono-detection.ConclusionMpco-detection with viral and/or bacterial pathogens is common in clinical practice. However, there are no apparent differences betweenMpmono-detection andMpco-detections in terms of clinical features and disease severity.

Aiming at the current number of recommended models in the house recommendation system model, the recommendation accuracy and user satisfaction do not meet the needs of users, this paper proposes a house recommendation model based on cosine similarity in the deep learning mode in grid environment. The model first uses the established grid environment, combined with the deep learning mode, through the collection of a large number of housing basic data samples and user feedback information, establishes a mathematical model of housing recommendation, and then integrates the cosine similarity into the recommendation model, through training and parameters. Adjust the model framework gradually. Finally, through the derivation and verification of the simulation experiment, the proposed recommendation model can efficiently recommend the housing information that meets the requirements for the user, and improve the recommendation accuracy and user satisfaction.

Aiming at the problems of low semantic recognition rate and inaccurate analysis in traditional ontology semantic similarity analysis, an ontology semantic similarity analysis method for deep learning mode under grid platform is proposed. The proposed method firstly combines the grid platform and deep learning to construct the ontology semantic analysis model, and then repeatedly trains the ontology semantic samples through the deep learning training mechanism, adjusts the parameters of the model, reconstructs the analysis model, and enables the computer to better understand the ontology. The semantic meaning, at the same time set the interrelationship between the ontology, and finally through the design of the software verification analysis model, the verification results show that the proposed ontology semantic analysis model can effectively improve the efficiency of analysis.

In order to effectively improve the accuracy of recommending information for users, this paper introduces the deep learning mode by means of grid environment, and proposes a recommendation system algorithm based on deep learning mode in grid environment. Firstly, the advantages and disadvantages of content recommendation and collaborative filtering recommendation algorithms are analyzed. Then, the deep learning mode is introduced, the recommended environment framework is established in the grid environment, and the recommendation algorithm is optimized. Finally, the simulation results show that the proposed recommendation algorithm can quickly establish a user recommendation list and effectively improve the recommendation accuracy.

In order to improve the accuracy of the project in the recommendation process, the article compares the existing content-based and collaborative filtering recommendation calculations, makes full use of the advantages of different algorithms, and proposes a collaborative filtering personalized recommendation algorithm based on deep learning optimization. Through the deep learning training of users' preferences, the corresponding parameters are adjusted to establish a personalized recommendation method. Through experimental verification, the proposed algorithm can effectively improve the quality of personalized recommendation.

This paper designs a cricket control system based on computer vision technology. This system is controlled by PID algorithm. The acquisition system is OpenMV camera, and the processing system is STM32 series MCU. The design is used to control the small ball to roll on the evenly distributed smooth square plate, so that the ball can be removed on the square plate without any external force except for the self-gravity, the support force of the plate and the friction force. Various specified requirements are fulfilled between circular areas of 3 cm in outer diameter. In this design, the image data is collected by the camera on the smooth flat panel range, and the collected data is transmitted to the STM32 core board through the serial port for processing, and the center coordinates of the hard ball are found through the processed data, and the coordinates are adjusted according to the set destination coordinates and the actual coordinates. PID (proportional-integral-derivative) controls the steering gear to rotate a certain angle to control the hard ball to achieve the relevant operational requirements.

Background Knee osteoarthritis (KOA) seriously reduces quality of life and is a major threat to the health of the middle-aged and elderly. This study aimed to assess the efficacy of Miao crossbow needle therapy vs. acupuncture for KOA therapy. Methods This multicenter, randomized controlled trial was performed at three hospitals between April 2016 and December 2016. The patients were randomized to receive crossbow needle (CN) or acupuncture (AT). All treatments were completed within 46 days. Evaluation of treatment was conducted on the 46th, 62nd, and 77th days. The primary endpoint was change of Western Ontario and McMaster Osteoarthritis Index (WOMAC) score on the 46th day. The secondary endpoints included WOMAC score, the Lysholm knee score, the Japanese Orthopedic Association (JOA) knee score, visual analog scale (VAS), and the MOS 36-item short-form health survey (SF-36), on the 46th, 62nd, and 77th day. Results Finally, data of 301 participants were analyzed for the efficacy of treatment. Compared with AT, there was a larger change of WOMAC score in the CN group after treatment [− 25.0 (95% CI − 27.0, − 23.0) vs. − 18.8 (95% CI − 20.8, − 16.9), P P = 0.008, P = 0.003, P P = 0.03) and JOA score (P = 0.013) were higher and the VAS score (P = 0.011) was lower on the 77th day. Conclusion Both Miao crossbow needle therapy and acupuncture reduced the WOMAC score. Miao crossbow needle therapy can be an alternative method for treating patients with knee osteoarthritis. Trial registration ChiCTR, ChiCTR-INR-16008032. Registered on 12 March 2016.

The establishment of a waiting area in an intersection with a complex traffic flow is investigated. With the adoption of the basic conditions of a waiting area, capacity and delay models are established to examine the differences in the capacities of and delays in an intersection with and without a waiting area. Signal control parameters, such as green-light interval time and effective green-light time, are also studied. The increase in capacity and reduction in delay from the setting of a waiting area are deduced by using accurate mathematical equations. With the use of actual intersections as examples, traffic operation efficiency was analyzed through simulation. Simulation results can be summarized as follows: generally, under high flow rate and saturation conditions, setting a straight waiting area can adequately utilize the intersection spare space, improve usage efficiency of straight lanes, and maximally realize reciprocal transformation between time and space. Consequently, the capacity of...

with the emergence of artificial intelligence technology, it is possible for financial robots to replace traditional accounting work. The paper analyzes the influence of artificial intelligence technology on accounting practice, accounting personnel and professional orientation, and puts forward the training direction of accounting talents.

Based on measurement and analysis of stone processing workshop dust source, stone processing dust treatment scheme is proposed, and the cloth bag dust remover and a set of dust removal system is introduced in this paper. In addition, the amount of gas filtration, filtration velocity, and filter bag expanded area and other parameters were calculated. Eventually, result shows that the system can change environment of stone production to get cleaning.

In the process of stone cutting and polishing, a large amount of high turbidity water is produced, which causes the waste of water resources and the pollution of the environment. The traditional treatment process is optimized, taking problems occurred in sewage treatment process by small and medium-sized enterprises and the existing technology and method into consideration. According to the production process of stone, the amount of waste water generated every day is calculated, which provides a theoretical basis for the sedimentation tank optimization and design. The necessity of stone wastewater reuse is illustrated.

The aim of the study was to investigate the protective effects of sodium hydrosulfide (NaHS), a H2S donor, against hypoxia-induced injury in human umbilical vein endothelial cells (HUVECs) and also to look into the possible mechanisms by which H2S exerts this protective effect. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and scratch wound healing assay were chosen to measure the cell viability and migration-promoting effects. The fluorescent probe, DCFH-DA and 5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethyl-imidacarbocyanine iodide (JC-1) were applied to detect the reactive oxygen species (ROS) level and mitochondrial membrane potential (ΔΨm). Furthermore, western blots were used to measure the expressions of the apoptosis-related proteins. Under hypoxic conditions, 300 μM and 600 μM of H2S could protect HUVECs against hypoxia-induced injury, as determined by MTT assay. Following the treatment of 60 µM NaHS for 18 h, scratch wound healing assays indicated that the scratch became much narrower than control group. After treatment with 60 µM, 120 µM, and 600 µM NaHS, and hypoxia for 30 min, flow cytometry demonstrated that the ROS concentrations decreased to 95.08% ± 5.52%, 73.14% ± 3.36%, and 73.51% ± 3.05%, respectively, compared with the control group. In addition, the JC-1 assay showed NaHS had a protective effect on mitochondria damage. Additionally, NaHS increased Bcl-2 expression and decreased the expression of Bax, Caspase-3 and Caspase-9 in a dose-dependent way. Our results suggest that H2S can protect endothelial cells and promote migration under hypoxic condition in HUVECs. These effects are partially associated with the preservation of mitochondrial function mediated by regulating the mitochondrial-dependent apoptotic pathway.

The [ 2 ¯ 33 ] coplanar double-slip-oriented Cu single crystals were pre-fatigued up to a saturation stage and then uniaxially tensioned or compressed to fracture. The results show that for the specimen pre-fatigued at a plastic strain amplitude γpl of 9.2 × 10−4, which is located within the quasi-plateau of the cyclic stress-strain (CSS) curve, its tensile strength and elongation are coincidently improved, showing an obvious strengthening effect by low-cycle fatigue (LCF) training. However, for the crystal specimens pre-fatigued at a γpl lower or higher than the quasi-plateau region, due to a low pre-cyclic hardening or the pre-induction of fatigue damage, no marked strengthening effect by LCF training occurs, and even a weakening effect by LCF damage takes place instead. In contrast, the effect of pre-fatigue deformation on the uniaxial compressive behavior is not so significant, since the compressive deformation is in a stress state more beneficial to the ongoing plastic deformation and it is insensitive to the damage induced by pre-cycling. Based on the observations and comparisons of deformation features and dislocation structures in the uniaxially deformed [ 2 ¯ 33 ] crystal specimens which have been pre-fatigued at different γpl, the micro-mechanisms for the effect of pre-fatigue on the static mechanical behavior are discussed.

Background Alkaloids from Piper longum (PLA), extracted from P. longum, have potent anti-inflammatory effects. The aim of this study was to investigate whether PLA could protect dopaminergic neurons against inflammation-mediated damage by inhibiting microglial activation using a lipopolysaccharide (LPS)-induced dopaminergic neuronal damage rat model. Methods The animal behaviors of rotational behavior, rotarod test and open-field test were investigated. The survival ratio of dopaminergic neurons and microglial activation were examined. The dopamine (DA) and its metabolite were detected by high performance liquid chromatography (HPLC). The effects of PLA on the expression of interleukin (IL)-6, interleukin (IL)-1β and tumor necrosis factor (TNF)-α were detected by enzyme-linked immunosorbent assay (ELISA). Reactive oxygen species (ROS) and nitric oxide (NO) were also estimated. Results We showed that the survival ratio of tyrosine hydroxylase-immunoreactive (TH-ir) neurons in the substantia nigra pars compacta (SNpc) and DA content in the striatum were reduced after a single intranigral dose of LPS (10 μg) treatment. The survival rate of TH-ir neurons in the SNpc and DA levels in the striatum were significantly improved after treatment with PLA for 6 weeks. The over-activated microglial cells were suppressed by PLA treatment. We also observed that the levels of inflammatory cytokines, including TNF-α, IL-6 and IL-1β were decreased and the excessive production of ROS and NO were abolished after PLA treatment. Therefore, the behavioral dysfunctions induced by LPS were improved after PLA treatment. Conclusion This study suggests that PLA plays a significant role in protecting dopaminergic neurons against inflammatory reaction induced damage.

In modern forest measurement situation that lack of good accuracy, in order to meet the needs of precision forestry and realize the precise measurement of every single tree, this paper introduces an automate technology and software platform of forest measurement in fixed sample. This technology and method can give full play to the precision total station angle measurement range function and PDA data processing and storage function, which are based on the communication system programming development of total station and PDA with WinCE. This automation technology are used to gain 3d coordinate, tree height, DBH and crown of trunks, it can both realize the automation process of testing trees and ensure the accuracy of measurement, improving the work efficiency greatly.

Background This phase I study investigated the maximum tolerated dose (MTD), safety, pharmacokinetics and antitumor activity of ganetespib in patients with solid malignancies. Methods Patients were enrolled in cohorts of escalating ganetespib doses, given as 1 hour IV infusion, once weekly for 3 weeks, followed by a 1-week rest until disease progression or unacceptable toxicity. Endpoints included safety, pharmacokinetic and pharmacodynamic parameters and preliminary clinical activity. Results Fifty-three patients were treated at doses escalating from 7 to 259 mg/m2. The most common adverse events were Grade 1 and 2 diarrhea, fatigue, nausea or vomiting. Dose-limiting toxicities (DLT) observed were: one Grade 3 amylase elevation (150 mg/m2), one Grade 3 diarrhea and one Grade 3 and one Grade 4 asthenia (259 mg/m2). The MTD was 216 mg/m2 and the recommended phase 2 dose was established at 200 mg/m2 given IV at Days 1, 8, and 15 every 4 weeks. There was a linear relationship between dose and exposure. Plasma HSP70 protein levels remained elevated for over a week post treatment. Disease control rate (objective response and stable disease at ≥ 16 weeks) was 24.4%. Conclusions Ganetespib is well tolerated as a weekly infusion for 3 of every 4 weeks cycle. The recommended phase II dose is 200 mg/m2, and is associated with an acceptable tolerability profile. Trial registration NCT00687934

To orient at special environment (such as weak satellite signals or no signals areas of high-rise apartment buildings in the city areas tunnels underground space indoor and under canopy, and the disaster environment and the region that satellite navigation cannot reach) precision navigation, positioning and monitoring, to aim at breaking through technical problems of high-precision angular measurement and rapid long-range precision measurement, and developing the high-end Smart Station positioning system products of physical structure and data-processing integration, to realize high-precision, low-cost and real time localization and precise monitoring with engineering deformation in special environment. This paper adds the location baseline position monitoring and three-dimensional location and other special functions based on the increase of accuracy of photoelectric angle measuring distance; solution positioning in the special environment; to solve bottleneck technology issue of low efficiency low accuracy poor reliability and multi-factor interference in the special environment and realize high-precision, low-cost and real-time location and precise monitoring.

BackgroundIn addition to caudal subnucleus caudalis (Vc) of the spinal trigeminal complex, recent studies indicate that the subnuclei interpolaris/caudalis (Vi/Vc) transition zone plays a unique role in processing deep orofacial nociceptive input. Studies also suggest that glia and inflammatory cytokines contribute to the development of persistent pain. By systematically comparing the effects of microinjection of the antiinflammatory cytokine interleukin (IL)-10 and two glial inhibitors, fluorocitrate and minocycline, we tested the hypothesis that there was a differential involvement of Vi/Vc and caudal Vc structures in deep and cutaneous orofacial pain.ResultsDeep or cutaneous inflammatory hyperalgesia, assessed with von Frey filaments, was induced in rats by injecting complete Freund's adjuvant (CFA) into the masseter muscle or skin overlying the masseter, respectively. A unilateral injection of CFA into the masseter or skin induced ipsilateral hyperalgesia that started at 30 min, peaked at 1 d and lasted for 1–1 weeks. Secondary hyperalgesia on the contralateral site also developed in masseter-, but not skin-inflamed rats. Focal microinjection of IL-10 (0.006-1 ng), fluorocitrate (1 μg), and minocycline (0.1-1 μg) into the ventral Vi/Vc significantly attenuated masseter hyperalgesia bilaterally but without an effect on hyperalgesia after cutaneous inflammation. Injection of the same doses of these agents into the caudal Vc attenuated ipsilateral hyperalgesia after masseter and skin inflammation, but had no effect on contralateral hyperalgesia after masseter inflammation. Injection of CFA into the masseter produced significant increases in N-methyl-D-aspartate (NMDA) receptor NR1 serine 896 phosphorylation and glial fibrillary acidic protein (GFAP) levels, a marker of reactive astrocytes, in Vi/Vc and caudal Vc. In contrast, cutaneous inflammation only produced similar increases in the Vc.ConclusionThese results support the hypothesis that the Vi/Vc transition zone is involved in deep orofacial injury and suggest that glial inhibition and interruption of the cytokine cascade after inflammation may provide pain relief.

Background Controversy still exists in the indication and timing of surgical treatment of pulmonary hamartoma (PH). The objective of this study is to summarize the experience and the outcome of the surgical treatment for pulmonary hamartomas, and to assess the effectiveness and necessity of surgical therapy administered in patients with pulmonary hamatoma as well as clinical and pathological features and long-term follow-up results. Methods This retrospective report has reviewed a 20-years clinical history of surgical treatment for 39 patients with PH from 1985 to 2006. These thirty-nine patients underwent 40 operations as follows: wedge resection (23), enucleation (10), segmentectomy (3), lobectomy (3), and pneumonectomy (1). Results The PH occurred most frequently (78.1%) in the patients aged 40 to 60 years and the sex ratio (male/female) was 2.25/1 in our series. No postoperative death was encountered. One patient with pleural effusion was cured after thoracentesis. All of these 39 patients were proved with pathologic diagnosis of PH and the popcorn calcification was found in 6 cases before operation. In 38 cases having the mean follow-up of 7.3 years, a patient was operated thrice for regional recurrence. Conclusion Fast frozen section in operation is critical for acquire accurate pathological diagnosis. Due to potential trend of recurrence or malignance, patients with hamartoma should be submitted to a complete evaluation and a regular follow-up.