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Introduction Chronic rhinosinusitis with nasal polyps (CRSwNP) is a common and frequently occurring disease of the upper respiratory tract. The nasal instillation of the Gram‐negative (G−) bacterial product lipopolysaccharide (LPS) can induce not only acute sinusitis but also the development of CRSwNP in animal models. Nevertheless, the expression and distribution of LPS in patients with CRSwNP have not been investigated. And the study was to investigate the expression of LPS and its relationship with glucocorticoid receptors (GRs) in CRSwNP. Methods Multiple methods, including bacterial culture and immunohistochemistry, were used to detect and analyze nasal bacteria, plasma LPS content, and the levels of LPS and GR‐α/β, cluster of differentiation 68 (CD68), and myeloperoxidase (MPO) expression, as well as their relationship in CRSwNP. Results The number of G− bacteria and Escherichia coli (E. coli) was not significantly different between CRSwNP subjects and the controls. However, the positive rate of LPS was much higher than that of E. coli in CRSwNP subjects and was significantly higher in noneosinophilic CRSwNP subjects than in eosinophilic CRSwNP subjects. Moreover, the LPS levels were positively correlated with GR‐β but not GR‐α expression in CRSwNP. Immunofluorescence assays showed that LPS was mainly detected in CD68+ macrophages and MPO+ neutrophils, in addition to histiocytes, in CRSwNP. Conclusions Persistent LPS in CRSwNP can lead to unresolved mucosal inflammation, eventually leading to tissue remodeling and the development of CRSwNP. Our findings suggest that increased LPS content and possible resistance to glucocorticoids may be one of the important pathogenic mechanisms of G− bacteria in CRSwNP., We used bacterial culture and immunohistochemistry to find that the positive rate of lipopolysaccharide (LPS) was much higher than that of Escherichia coli in chronic rhinosinusitis with nasal polyps (CRSwNP), and the LPS levels were positively correlated with glucocorticoid receptor‐β (GR‐β) expression in CRSwNP. Our findings suggest increased LPS content and possible resistance to glucocorticoids may be one of the important pathogenic mechanisms of G‐negative bacteria in CRSwNP.

We demonstrate a handheld swept-source optical coherence tomography (OCT) system with a 400 kHz vertical-cavity surface-emitting laser (VCSEL) light source, a non-contact approach, and an unprecedented single shot 105° field of view (FOV). We also implemented a spiral scanning pattern allowing real-time visualization with improved scanning efficiency. To the best of our knowledge, this is the widest FOV achieved in a portable non-contact OCT retinal imaging system to date. Improvements to the FOV may aid the evaluation of retinal diseases such as retinopathy of prematurity, where important vitreoretinal changes often occur in the peripheral retina.

ObjectiveTo determine whether handheld widefield optical coherence tomography (OCT) can be used to document retinopathy of prematurity (ROP) stage while using scleral depression to improve peripheral views.DesignProspective observational studyParticipantsConsecutive neonates admitted to the neonatal intensive care unit (NICU) in a single academic medical center who also met criteria for ROP screening and consented for research imaging.MethodsScleral depression was combined with widefield OCT using an investigational 400-kHz, 55-degree field of view handheld OCT during ROP screening from October 28, 2020 to March 03, 2021.Main Outcome MeasuresAcquisition of en face and B-scan imaging of the peripheral retina to objectively assess early vitreoretinal pathology, including the demarcation between vascularized and anterior avascular retina, the presence of early ridge formation, and small neovascular tufts.ResultsVarious stages of ROP were detected using a rapid acquisition OCT system. In one neonate, serial OCT imaging over a five-week period demonstrated accumulation of neovascular tufts with progression to stage 3 ROP with extraretinal fibrovascular proliferation along the ridge. Videography of this technique is included in this report for instructional purposes.ConclusionsSerial examinations using widefield OCT and scleral depression may improve detection and documentation of ROP disease progression. Earlier detection of ROP-related proliferation may prevent vitreoretinal traction, retinal detachment, and blindness.

BackgroundAfter the scale-up of antiretroviral therapy (ART) for HIV infected people, increasing numbers of patients have pretreatment drug resistance (PDR). In this study, the prevalence of PDR was evaluated in adults initiating antiretroviral therapy in China.MethodsBlood samples were obtained from 1943 patients who initiated antiretroviral therapy (ART) in 2017 from 13 provinces or cities in China. Pol sequences were used to analyze drug resistance and construct transmission networks. Logistic regression model was used to estimate the potential factors associated with PDR.ResultsIn total, 1711 eligible patients (76.0% male; 87.8% aged ≥ 25 years) were included, of which 117 (6.8%) had PDR. The highest rates of PDR were 12.2% in Liangshan Prefecture of Sichuan and 9.3 and 8.9% in Dehong and Lincang Prefecture of Yunnan. A multivariate logistic regression analysis revealed that PDR was significantly higher among intravenous drug users (adjusted Odds Ratio (aOR) = 2.64, 95%CI: 1.57–4.44) and individuals from Liangshan, Dehong, and Lincang (aOR = 2.04, 95%CI: 1.26–3.30). In total, 754 sequences were used to generate 164 transmission networks. Five transmission networks had two or three sequences containing the same mutations, two networks contained subjects from Liangshan, and one network contained subjects from Dehong.ConclusionsOverall, the PDR prevalence was moderate, with a particularly high prevalence in areas with severe HIV epidemics. These results indicate the importance of continuous PDR monitoring in patients initiating antiretroviral therapy.

Optical coherence tomographic angiography (OCTA) can image the retinal blood flow but visualization of the capillary caliber is limited by the low lateral resolution. Adaptive optics (AO) can be used to compensate ocular aberrations when using high numerical aperture (NA), and thus improve image resolution. However, previously reported AO-OCTA instruments were large and complex, and have a small sub-millimeter field of view (FOV) that hinders the extraction of biomarkers with clinical relevance. In this manuscript, we developed a sensorless AO-OCTA prototype with an intermediate numerical aperture to produce depth-resolved angiograms with high resolution and signal-to-noise ratio over a 2 × 2 mm FOV, with a focal spot diameter of 6 µm, which is about 3 times finer than typical commercial OCT systems. We believe these parameters may represent a better tradeoff between resolution and FOV compared to large-NA AO systems, since the spot size matches better that of capillaries. The prototype corrects defocus, astigmatism, and coma using a figure of merit based on the mean reflectance projection of a slab defined with real-time segmentation of retinal layers. AO correction with the ability to optimize focusing in arbitrary retinal depths – particularly the plexuses in the inner retina – could be achieved in 1.35 seconds. The AO-OCTA images showed greater flow signal, signal-to-noise ratio, and finer capillary caliber compared to commercial OCTA. Projection artifacts were also reduced in the intermediate and deep capillary plexuses. The instrument reported here improves OCTA image quality without excessive sacrifice in FOV and device complexity, and thus may have potential for clinical translation.

Sensorless adaptive optics optical coherence tomography (AO-OCT) is a technology to image retinal tissue with high resolution by compensating ocular aberrations without wavefront sensors. In this Letter, a fast and robust hill-climbing algorithm is developed to optimize five Zernike modes in AO-OCT with a numerical aperture between that of conventional AO and commercial OCT systems. The merit function is generated in real time using graphics processing unit while axially tracking the retinal layer of interest. A new method is proposed to estimate the largest achievable field of view for which aberrations are corrected uniformly in sensorless AO-OCT.

A multi-dopant material system for polymer-stabilized (PS) blue phase liquid crystal (BPLC) is proposed. The driving capacity of PS-BPLC can be improved by doping N-vinylpyrrollidone (NVP) and polyaniline functionalized graphene (G-PANI). With the dopant optimization, the Kerr constant is increased by 68%, and the response time, hysteresis and residual birefringence have no degradation.

A combined fringe and in-plane switching (FIS) mode single-cell-gap transflective display using blue-phase liquid crystal (BPLC) is proposed. The structure makes use of the space above the pixel electrodes of this FIS mode as reflective regions and the space between pixel electrodes as transmissive regions. The structure which can be optimized to balance the optical phase retardation between transmissive region and reflective region with simple fabrication process exhibits relatively high optical efficiency and well matched voltage of transmittance and reflectance curves.

A novel fabrication method using holographic exposure for switchable blue phase (BP) liquid crystal (LC)/polymer Fresnel lens was demonstrated. The Fresnel pattern can be achieved by interfering a planar and a spherical wave fronts with a 532-nm laser. The BPLC/polymer Fresnel lens can thus be fabricated without photo mask and controlled with a simpler driving compared with the conventional LC Fresnel lens.

Lactoferrin (LF) can downregulate allergic airway inflammation in asthma. However, the in vivo effect of exogenous LF on allergic rhinitis (AR), a disease attributed to airway inflammation, has yet to be determined. We investigated the effect of intranasal administration recombinant human (rh) LF and its underlying mechanisms on AR in BALB/c mice. Multiple parameters of allergic responses were evaluated to determine the effect of rhLF. We found that the number of eosinophils and goblet cells, as well as mRNA and protein expression of type 2 helper T (Th2), Th17 and regulatory T (Treg) cells in the nasal cavity, was significantly upregulated in AR mice compared with the controls, Conversely, administration of rhLF prior to or after intranasal ovalbumin challenge markedly downregulated these same parameters. Th1-specific mRNA and protein expression in the nasal cavity of the controls was not different from that in AR mice, but expression significantly increased with rhLF treatment. The mRNA and protein expression of endogenous LF in the nasal cavity was significantly downregulated in AR mice compared with the controls. However, after rhLF treatment, endogenous LF mRNA and protein expression was significantly upregulated. Exogenous rhLF inhibited allergic inflammation in AR mice, most likely by promoting the endogenous LF expression and skewing T cells to a Th1, but not a Th2 and Th17 phenotype in the nasal mucosa. Our findings suggest that rhLF treatment may be a novel therapeutic approach for prevention and treatment AR.

The article takes listed companies as research samples. Firstly, it selects 36 ST or *ST companies listed in Shanghai and Shenzhen Stock Exchange Market, who received special treatment during 2007 to 2009 for the first time and it also chooses another 36 normal companies as paired ones. Then, after using Factor analysis for identifying indexes, the paper go on with utilizing logistic to structure a financial long-term warning model. To verify the effectiveness of the model, the paper selects another 12 financial crisis companies and 12 financial fit companies to test. The results come out to show that establishing an effective long-term financial early-warning system helps enterprises to avoid financial crisis.

A In Plane Switching (IPS) mode single-cell-gap transflective display using blue-phase liquid crystal (BPLC) is proposed. The novel structure makes use of the space above the electrodes of the IPS structure which contributes little to the total transmittance due to weak horizontal electric field as reflective region and the space between the electrodes as transmissive region. The shape of the electrodes is optimized to balance the phase retardation between the transmissive region and reflective region. This transflective structure exhibits relatively high optical efficiency and well matched driving voltage of both transmittance and reflectance curves.

Blue phase liquid crystals(BPLCs) show fast response time and macroscopic optical isotropy due to their double twist cylinder structure.With polymer stabilization,the thermal stability of BPLCs can be improved dramatically.However,it induces some problems to the electro-optic properties of BPLCs material such as high driving voltage,hysteresis and so on.In this paper,different concentrations of chiral dopant,polymer network of the BPLC,and temperature effect are investigated to improve the electro-optic properties of BPLCs and analyze the mechanism that affect the electro-optic properties of the BPLCs device.

Chronic rhinosinusitis (CRS) is a heterogeneous disease, with varying immunological and histopathological features. The CC chemokine ligand 19 (CCL19) can stimulate T cells and antigen-presenting cells into secondary lymphoid node formation, as observed in allergic rhinitis, inflammatory bowel disease and other inflammatory disorders. The purpose of this study was to investigate the expression and significance of CCL19 in CRS. Samples of uncinate process mucosa or nasal polyps were taken from patients with CRS (without or with nasal polyps) and normal controls during surgery. Hematoxylin and eosin, periodic acid Schiff and Masson trichrome staining were used for analysis of the nasal polyps. Western blot and immunofluorescence were used to detect CCL19 expression in the nasal polyps and normal nasal mucosa tissues. Spearman correlation analysis was used to analyze the association between CCL19 and blood eosinophil counts. The results showed that CCL19 protein levels in CRS (with or without nasal polyps) were significantly upregulated compared with those in controls. CCL19 expression in eosinophilic CRS was significantly higher than in non-eosinophilic CRS. CCL19 expression in fibroinflammatory and edematous type CRS with nasal polyps was higher than in controls; the upregulation was greater in the edematous type. Immunofluorescence assays showed that CCL19 was mainly expressed by CD68+ macrophages. Spearman correlation analysis demonstrated a positive correlation between CCL19 and blood eosinophils. The upregulation of CCL19 in CRS may play a protective role by limiting eosinophil infiltration and the extent of edema to exert anti-inflammatory and immunomodulatory effects.

Historically, a sectoral approach (based on the industrial, transportation, commercial, and residential sectors) has shaped the way we frame and analyze issues of energy conservation and CO 2 mitigation. This sectoral categorization, however, is limited in its capacity to reveal the total impacts of consumer activities on energy use and its related environmental impacts. In this paper, we propose an alternative paradigm, called the Consumer Lifestyle Approach (CLA), to explore the relationship between consumer activities and environmental impacts in the US. Estimates based on our methodology reveal that more than 80% of the energy used and the CO 2 emitted in the US are a consequence of consumer demands and the economic activities to support these demands. Direct influences due to consumer activities (home energy use and personal travel) are 4% of the US GDP, but account for 28% and 41% of US energy use and CO 2 emissions, respectively. Indirect influences (such as housing operations, transportation operations, food, and apparel) involve more than twice the direct energy use and CO 2 emissions. Characterization of both direct and indirect energy use and emissions is critical to the design of more effective energy and CO 2 emission policies. It may also help erode the false dichotomy of “them versus us” (industrial polluters versus consumers) references to the locus of responsibility for control of energy use and CO 2 emissions.

The scanning liquid crystal (LC) prism type 3D displays may solve the resolution degradation problem but introduce another issue, dynamic crosstalk. To improve the 3D perception, a novel impulsive driving backlight method with multi-electrode driving structure scanning LC prism is proposed. With this method, the dynamic crosstalk can be reduced by 35.2%.

novel holographic exposure technique for switchable cylindrical structure LC/polymer Fresnel lens is proposed. The one dimension Fresnel pattern can be achieved by interfering two planar and spherical wave fronts with a shifting slit. In this simple fabrication process, the Fresnel pattern is easy to control and the mask is not needed compare to the conventional process. The optical property of the lens is confirmed.

Background Chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) and without nasal polyps (CRSsNP) is reported to be different in inflammatory patterns of the sinonasal mucosa in white patients. Studies in nonwhite populations may further be helpful to understand the pathogenic mechanisms of CRS. Objective To investigate the immunopathologic profiles of CRSwNP and CRSsNP in adult Chinese. Methods Histologic characteristics of surgical samples were analyzed in 50 controls, 94 CRSsNP patients, and 151 CRSwNP patients. Tissue samples from 17 controls, 36 CRSsNP patients, and 45 CRSwNP patients were stained for CD3, CD4, CD8, CD20, CD68, myeloperoxidase, and dendritic cell lysosome-associated membrane protein. Expression profiles of transcription factors of T-cell subsets in relation to cytokines and a marker of natural killer T cell (Vα24) were examined by means of quantitative RT-PCR. Results Over half of CRSwNP patients presented noneosinophilic inflammation. CRSwNP had a higher number of eosinophils, plasma cells, and CD3 + , CD8 + , CD20 + , and CD68 + cells and a lower myeloperoxidase expression rate than CRSsNP. Expression levels of transcription factors and cytokines of T H 1/T H 2/T H 17 were increased, whereas the expression rate of Forkhead box p3 and TGF-β1 was decreased in both CRSsNP and CRSwNP compared with controls. Comparing CRSsNP and CRSwNP, CRSsNP had higher levels of IFN-γ expression, whereas only eosinophilic CRSwNP demonstrated an enhanced expression of GATA-3 and IL-5. Compared with noneosinophilic CRSwNP, an exaggerated T H 2/T H 17 reaction and Vα24 expression were found in eosinophilic CRSwNP. Conclusion Both Chinese CRSsNP and CRSwNP patients demonstrate impaired regulatory T cell function and enhanced T H 1/T H 2/T H 17 responses. CRSsNP is confirmed to be a predominant T H 1 milieu, whereas T H 2 skewed inflammation with predominant T H 17 reactions, and infiltration of natural killer T cells can be demonstrated only in eosinophilic CRSwNP, but not in noneosinophilic CRSwNP.

Background Interleukin-10 (IL-10) has an important anti-inflammatory and immunoregulatory function, and its expression is negatively correlated with the development and severity of allergic rhinitis (AR). However, the in vivo effects of exogenous IL-10 on AR have not been studied and the mechanisms underlying the effects of IL-10 have not been fully understood. Here, we investigated the effects of intranasal administration of recombinant mouse (rm) IL-10 on the expression of Th responses and local IL-10 in a mouse model of AR induced by ovalbumin. Results Administration of rmIL-10 during challenge significantly reduced the number of eosinophils and mast cells, as well as Type 2 helper T (Th2) and Th17 cell related cytokine and transcription factor levels in the nasal mucosa and nasal lavage fluid in AR mice. The rmIL-10 treatment significantly inhibited the number of IL-10-positive cells and IL-10 mRNA expression in the nasal mucosa in AR mice. Conclusion Our results show that exogenous IL-10 administrated in challenge phase alleviates nasal allergic inflammation in AR mice, most likely by inhibiting Th2 and Th17 responses. It can also inhibit local IL-10 levels in the nasal mucosa. Our findings indicate that IL-10 may have the potential as an inhibitor of AR.