5 results on '"S. Palea"'
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2. Comparison of the relaxant effects of alfuzosin, phentolamine and sildenafil on rabbit isolated corpus cavernosum
- Author
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S. Palea and M. Barras
- Subjects
medicine.medical_specialty ,Contraction (grammar) ,Sildenafil ,business.industry ,Urology ,Antagonist ,Erectile tissue ,Nitric oxide ,chemistry.chemical_compound ,Endocrinology ,medicine.anatomical_structure ,Phentolamine ,chemistry ,Internal medicine ,medicine ,business ,Phenylephrine ,Alfuzosin ,medicine.drug - Abstract
OBJECTIVE To compare the direct relaxant effects of alfuzosin, phentolamine and sildenafil in rabbit isolated corpus cavernosum (CC) pre-contracted with phenylephrine or KCl. MATERIALS AND METHODS Penile erectile tissue was obtained from male New Zealand White rabbits (22–26 weeks old). The CC was cut into longitudinal strips and mounted under 2 g resting tension in 5-mL jacketed organ baths containing a modified Krebs solution bubbled with 95% O2, 5% CO2 and maintained at 37 °C. Tissue strips were pre-contracted by 60 mmol/L KCl or 10 µmol/L phenylephrine. After obtaining a stable plateau of contractions, test compounds were added to the organ bath. The relaxant potencies were expressed as the percentage of inhibition of the plateau of contraction induced by 10 µmol/L phenylephrine. RESULTS Alfuzosin showed a concentration-dependent relaxing effect on rabbit CC pre-contracted by 10 µmol/L phenylephrine, with a mean (sd) pIC50 of 7.64 (0.06). The relaxant effect was unaffected by pre-incubation with 100 µmol/L Nω-nitro-l-arginine methyl ester (L-NAME). Phentolamine had a potency similar to alfuzosin, with a pIC50 of 7.44 (0.08). Both alfuzosin and phentolamine were completely ineffective on the plateau of contraction induced by 60 mmol/L KCl. In contrast to alfuzosin, sildenafil was equipotent in relaxing the rabbit CC against each contractile agent, with pIC50 values of 7.25 (0.09) and 7.23 (0.22) with 10 µmol/L phenylephrine and 60 mmol/L KCl, respectively. The relaxant response to sildenafil was partly blocked by pretreatment with 100 µmol/L L-NAME, with pIC50 values of 7.94 (0.09) and 6.63 (0.32) without and with L-NAME, respectively. Sildenafil, incubated for 45 min at 10 µmol/L, had no relaxant effect on the resting tension of the preparation or on the concentration-response curve to phenylephrine. CONCLUSIONS The direct relaxant effect of alfuzosin is mediated through α1-adrenoceptor blockade. The relaxations induced by phentolamine and alfuzosin are independent of nitric oxide, whereas those induced by sildenafil are, at least partly, sensitive to L-NAME and a selective soluble guanylate cyclase inhibitor, indicating the involvement of nitric oxide and soluble guanylate cyclase. Alfuzosin and phentolamine effectively counteract α1-adrenoceptor-mediated contractions of rabbit CC. If valid for human CC, such an effect may contribute to an improved erectile function in patients treated for benign prostatic hyperplasia.
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- 2003
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3. Experimental acute kidney injury
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A. Kuma, S. Yamada, T. Miyamoto, R. Serino, M. Tamura, Y. Otsuji, K. Kohno, W. Y. Cho, M.-G. Kim, S.-K. Jo, H. K. Kim, J. C. Jado, B. Humanes, V. Lopez-Parra, S. Camano, J. M. Lara, E. Cercenado, A. Tejedor, A. Lazaro, M. Jansen, G. Castellano, A. Stasi, A. Intini, M. Gigante, A. M. Di Palma, C. Divella, G. S. Netti, C. Prattichizzo, P. Pontrelli, A. Crovace, F. Staffieri, E. Fiaccadori, N. Brienza, G. Grandaliano, G. B. Pertosa, L. Gesualdo, K. Xanthopoulou, I. Tsouchnikas, G. Ouzounidis, G. Kokaraki, R. Lagoudaki, C. Simeonidou, G. Karkavelas, E. Spandou, D. Tsakiris, K. Kallaras, R. Schneider, M. Meusel, B. B. Betz, C. Held, K. Moller-Ehrlich, M. Buttner-Herold, C. Wanner, G. Michael, C. Sauvant, A. Hosszu, Z. Antal, J. Hodrea, S. Koszegi, N. F. Banki, L. Wagner, L. Lenart, A. Vannay, A. J. Szabo, A. Fekete, A. Michael, T. Faga, M. Navarra, M. Andreucci, S. Lemoine, B. Pillot, M. Rabeyrin, A. Varennes, M. Ovize, L. Juillard, L. Gomes Santana, W. Silva Almeida, N. Schor, M. Watanabe, C. D. Fonseca, E. A. Pessoa, M. H. Mendonca, S. M. Fernandes, F. T. Borges, M. F. Vattimo, C. P. C. Ow, F. Tassone, M. P. Koeners, S. C. Malpas, R. G. Evans, C. Alfarano, M.-A. Guardia, P. Lluel, S. Palea, G.-H. Young, V.-C. Wu, D. E. Choi, J. Y. Jeong, Y. K. Chang, S. Chung, K. R. Na, S. S. Kim, K. W. Lee, Y. Yang, L. Zhang, P. Fu, Y. Zhao, X. Zhang, I. Jadot, A.-E. Decleves, V. Colombaro, B. Martin, V. Voisin, I. Habsch, E. Deprez, J. Nortier, N. Caron, T. Iwakura, T. Fujikura, N. Ohashi, H. Yasuda, Y. Fujigaki, C. F. Vasco, M. D. F. F. Vattimo, J. Draibe, Y. Y ld r m, O. Aba, Z. Y lmaz, A. K. Kadiroglu, M. E. Y lmaz, M. Gul, A. Ketani, L. Colpan, L. B. d. M. Neiva, J. Suller Garcia, A. S. d. Oliveira, M. A. Naves, R. P. L. Van Swelm, J. F. M. Wetzels, V. G. M. Verweij, C. M. M. Laarakkers, J. C. L. M. Pertijs, D. W. Swinkels, R. Masereeuw, J. Sereno, P. Rodrigues-Santos, H. Vala, P. Rocha-Pereira, J. Fernandes, A. Santos-Silva, F. Teixeira, F. Reis, A. Altuntas, H. R. Yilmaz, E. Uz, M. Demir, A. Gokcimen, D. S. Bayram, O. Aksu, M. T. Sezer, K. H. Yang, Y. J. Jung, D. Kim, A. S. Lee, S. Lee, K. P. Kang, S. K. Park, W. Kim, N. A. Junglee, C. R. Searell, M. M. Jibani, J. H. Macdonald, C.-C. Wu, C.-C. Chen, K.-C. Lu, Y.-F. Lin, G. R. Estrela, F. Wasinski, R. Pereira, D. Malheiros, N. O. S. Camara, R. C. Araujo, M. F. Ramos, C. d. S. Passos, C. V. Razvickas, F. Borges, M. Ormanji, E. Plotnikov, M. Morosanova, I. Pevzner, L. Zorova, V. Manskikh, M. Skulachev, V. Skulachev, D. Zorov, C. F. Pinto, M. Vattimo, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Institut National de la Recherche Agronomique (INRA)
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0303 health sciences ,Transplantation ,medicine.medical_specialty ,business.industry ,[SDV]Life Sciences [q-bio] ,030232 urology & nephrology ,Acute kidney injury ,Urology ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Nephrology ,Medicine ,business ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology - Abstract
International audience
- Published
- 2014
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4. INVOLVEMENT OF SPINAL NK 1 AND OPIOIDS RECEPTORS IN MODULATING THE INHIBITORY EFFECT OF CAPSAICIN ON MICTURITION REFLEX IN THE ACUTE SPINALIZED GUINEA PIG
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S. Palea and C. Pietra
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medicine.drug_class ,business.industry ,Urology ,Antagonist ,(+)-Naloxone ,Pharmacology ,Spinal cord ,Guinea pig ,chemistry.chemical_compound ,Phentolamine ,medicine.anatomical_structure ,chemistry ,Capsaicin ,Opioid receptor ,Anesthesia ,Reflex ,medicine ,business ,medicine.drug - Abstract
Purpose: The aim of this work was to study the role of capsaicin-sensitive afferent fibers in modulating the micturition reflex at spinal level in urethane-anesthetized guinea pigs after spinal cord transection at level T3-T4.Materials and Methods: The intravesical effect of capsaicin was investigated in a series of cystometrograms performed in intact and spinalized animals.Results: In both intact and spinalized animals capsaicin, at 30 micro M, induced a significant increase of volume threshold only, whereas at 100 micro M it induced a complete inhibition of the spinal micturition reflex in 60% and 85% of the animals tested, respectively. This capsaicin inhibitory effect (CIE) was unaffected by intravenous phentolamine and propranolol (0.5 and 1 mg./kg., respectively), indomethacin at 100 nmoles intrathecally (i.t.), the CGRP receptor antagonist hCGRP8-37 (3 nmoles i.t.) and the NK2 receptor selective antagonist GR 94800 (1 nmol. i.t.). However, both naloxone (30 micro g. i.t.) and the NK1 antago...
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- 1999
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5. Characterization of the inhibitory response to intravesical capsaicin during cystometry in guinea-pig with spinal cord transection
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L. Zivian, S. Palea, and C. Pietra
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medicine.diagnostic_test ,Endocrine and Autonomic Systems ,business.industry ,Cystometry ,General Medicine ,Inhibitory postsynaptic potential ,Guinea pig ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Endocrinology ,Spinal cord transection ,Neurology ,chemistry ,Capsaicin ,Anesthesia ,medicine ,business - Published
- 1993
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