Objective: To assess whether knowledge of Tuskegee, the U.S. Immigration and Customs Enforcement (ICE) agency's detainment of children, and satisfaction with the George Floyd death investigation were associated with trust in actors involved in the development and distribution of coronavirus vaccines. Data Sources and Study Setting: National survey with a convenience sample of Black (n = 1019) and Hispanic (n = 994) adults between July 1 and 26, 2021. Study Design: Observational study using stratified adjusted logistic regression models to measure the association between ratings of the trustworthiness of actors involved in the development and distribution of coronavirus vaccines. Principal Findings: Among Black respondents, lower satisfaction with the George Floyd death investigation was associated with lower trustworthiness ratings of pharmaceutical companies (ME: -0.09; CI: -0.15, 0.02), the FDA (ME: -0.07; CI: -0.14, -0.00), the Trump Administration (ME: -0.09; CI: -0.16, -0.02), the Biden Administration (ME: -0.07, CI: -0.10, 0.04), and elected officials (ME: -0.10, CI: -0.18, -0.03). Among Hispanic respondents, lower satisfaction was associated with lower trustworthiness ratings of the Trump Administration (ME: -0.14, CI: -0.22, -0.06) and elected officials (ME: -0.11; CI: -0.19, -0.02). Greater knowledge of ICE's detainment of children and families among Hispanic respondents was associated with lower trustworthiness ratings of state elected officials (ME: -0.09, CI: -0.16, 0.01). Greater knowledge of the US Public Health Service Study of Syphilis in Tuskegee was associated with higher trustworthiness ratings of their usual source of care (ME: 0.09; CI: 0.28,0.15) among Black respondents (ME: 0.09; CI: 0.01,0.16). Conclusions: Among Black respondents, lower satisfaction with the George Floyd death investigation was associated with lowered levels of trust in pharmaceutical companies, some government officials, and administrators; it was not associated with the erosion of trust in direct sources of health care delivery, information, or regulation. Among Hispanic respondents, greater knowledge of the ICE detainments was associated with lower trustworthiness ratings of elected state officials. Paradoxically, higher knowledge of the Study of Syphilis in Tuskegee was associated with higher trustworthiness ratings in usual sources of care. KEYWORDS health equity, questionnaire design, social determinants of health, survey research What is known on this topic * Scholars attributed low early uptake of the COVID-19 vaccines among Black and Hispanic Americans to medical mistrust. * Medical mistrust is associated with experiences with racism and discrimination in the health care system and beyond. * US media and the scientific community have named perceptions of historical and contemporary atrocities as contributors to low levels of trust of medical professionals among Black and Hispanic communities. What this study adds * Black respondents who were less satisfied with the George Floyd death investigation had lower trustworthiness ratings of pharmaceutical companies and government, but not sources of health care information, regulation, or delivery (i.e., usual care source, vaccine clinics, the FDA). * Hispanic respondents with greater knowledge of the ICE's detainment of children and families had lower trustworthiness ratings of state elected officials. * State-sanctioned structural violence in racially and ethnically minoritized communities is associated with racially and ethnically concordant lower levels of trust in COVID vaccinerelated actors., 1 | INTRODUCTION The low early uptake of coronavirus vaccines among Black and Hispanic populations in the United States has been attributed to widespread medical mistrust rather than the misconduct [...]