1. Identifying Circulating MicroRNA in Kawasaki Disease by Next-Generation Sequencing Approach
- Author
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Kuo-Wang Tsai, Ken-Pen Weng, Luo-Ping Ger, Ching-Feng Cheng, Kuang-Jen Chien, and Shih-Hui Huang
- Subjects
0301 basic medicine ,Microbiology (medical) ,QH301-705.5 ,Mucocutaneous Lymph Node Syndrome ,Microbiology ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,law ,030225 pediatrics ,microRNA ,medicine ,Humans ,Circulating MicroRNA ,Biology (General) ,Molecular Biology ,Polymerase chain reaction ,Kawasaki disease ,circulating biomarker ,biology ,business.industry ,Gene Expression Profiling ,fungi ,High-Throughput Nucleotide Sequencing ,food and beverages ,General Medicine ,medicine.disease ,humanities ,MicroRNAs ,stomatognathic diseases ,030104 developmental biology ,ROC Curve ,Apoptosis ,biological sciences ,Immunology ,Disease Progression ,biology.protein ,Biomarker (medicine) ,Antibody ,Signal transduction ,business ,Biomarkers - Abstract
Kawasaki disease (KD) typically occurs in children aged under 5 years and can cause coronary artery lesions (CALs). Early diagnosis and treatment with intravenous immunoglobulin can reduce the occurrence of CALs, therefore, identifying a good biomarker for diagnosing KD is essential. Here, using next-generation sequencing in patients with recurrent KD, those with viral infection, and healthy controls, we identified dysregulated circulating microRNAs as diagnostic biomarkers for KD. Pathway enrichment analysis illustrated the putative role of these miRNAs in KD progression. Their expression levels were validated using real-time polymerase chain reaction (qPCR). Fifteen dysregulated circulating miRNAs (fold changes >, 2 and <, 0.5) were differentially expressed in the recurrent KD group compared with the viral infection and control groups. These miRNAs were significantly involved in the transforming growth factor-β, epithelial–mesenchymal transition, and cell apoptosis signaling pathways. Notably, their expression levels were frequently restored after intravenous immunoglobulin treatment. Among the candidates, miR-24-3p expression level was significantly higher in patients with recurrent KD compared with healthy controls or viral infection controls (p <, 0.001). Receiver operating characteristic analysis revealed that high miR-24-3p expression levels may be a potential biomarker for KD diagnosis. In conclusion, we identified miR-24-3p significantly higher in KD patients, which may be a potential diagnostic biomarker for KD.
- Published
- 2021